Abstract: Compositions, kits and methods are described that comprise one or more constructs, each construct comprising a ligand attached or conjugated to a polymer construct, e.g., an oligonucleotide sequence, by a linker, each ligand binding specifically to a single target located in or on the surface of a cell. The polymer construct comprises a) an Amplification Handle; b) a Barcode that specifically identifies a single ligand; c) an optional Unique Molecular Identifier that is positioned adjacent to the Barcode on its 5? or 3? end; and d) an Anchor for hybridizing to a complementary sequence, e.g., for generation of a double-stranded oligonucleotide. These compositions are used in methods, including high throughput methods, for detecting one or more targets or epitopes in a biological sample. These compositions are also used in a high throughput method for characterizing a cell by simultaneous detection of one or more epitopes located in or on the cell and its transcriptome.

Abstract: Methods are provided for analyzing one or more genetic samples, comprising procuring one or more genetic samples comprising genetic material from one or more individuals and sequencing the genetic material using non-targeted, ultra-low coverage sequencing to obtain genetic information for individual associated with the one or more genetic samples. Personal and genetic information associated with the individuals is stored in a database for retrieval and manipulation.

Abstract: A genomic data processing system can be configured to process next-generation sequencing information. The genomic data processing system described herein can accurately detect mutations in nucleic acid (e.g., cell free DNA (cfDNA) sequence reads associated with plasma nucleic acid samples. The genomic data processing system of the present disclosure also detects microsatellite instability in nucleic acid sequence reads with a higher degree of sensitivity compared to existing genomic data processing systems.

Abstract: Methods for preparing and utilizing tunable electrostatic capture (“TEC”) ligands that have an ionizable function are provided. The electrostatic nature of the ionizable functionality of the TEC ligands can be “tuned” or adjusted to either reversibly bind or release a desired target anion, such as a biomolecule, by varying the pH and/or the ionic strength of the binding conditions and release conditions. The TEC ligands can be bound to a solid support to form TEC binding surfaces. TEC surfaces, ligands, solid supports and the accompanying methods and buffer systems can be used to isolate polyanions, such as nucleic acids, from materials, for example in a size-selective manner.

Abstract: Compositions, kits and methods are described that comprise one or more constructs, each construct comprising a ligand attached or conjugated to a polymer construct, e.g., an oligonucleotide sequence, by a linker, each ligand binding specifically to a single target located in or on the surface of a cell. The polymer construct comprises a) an Amplification Handle; b) a Barcode that specifically identifies a single ligand; c) an optional Unique Molecular Identifier that is positioned adjacent to the Barcode on its 5? or 3? end; and d) an Anchor for hybridizing to a complementary sequence, e.g., for generation of a double-stranded oligonucleotide. These compositions are used in methods, including high throughput methods, for detecting one or more targets or epitopes in a biological sample. These compositions are also used in a high throughput method for characterizing a cell by simultaneous detection of one or more epitopes located in or on the cell and its transcriptome.

Abstract: Methods are provided for analyzing one or more genetic samples, comprising procuring one or more genetic samples comprising genetic material from one or more individuals and sequencing the genetic material using non-targeted, ultra-low coverage sequencing to obtain genetic information for individual associated with the one or more genetic samples. Personal and genetic information associated with the individuals is stored in a database for retrieval and manipulation.

Abstract: This disclosure is directed, in part, toward preparing and utilizing tunable electrostatic capture (“TEC”) ligands that have an ionizable function. The electrostatic nature of the ionizable functionality of the TEC ligands can be “tuned” or adjusted to either reversibly bind or release a desired target anion, such as a biomolecule, by varying the pH and/or the ionic strength of the binding conditions and release conditions. The TEC ligands can be bound to a solid support to form TEC binding surfaces. TEC surfaces, ligands, solid supports and the accompanying methods and buffer systems can be used to isolate polyanions, such as nucleic acids, from materials, for example in a size-selective manner.