Abstract: Provided herein is technology related to the chemical modification and purification of DNA. Specifically, the technology provides methods for performing a bisulfite conversion reaction on small amounts of single-stranded, fragmented DNA and performing the subsequent desulfonation and purification steps on magnetic beads.

Abstract: Disclosed methods relate to producing an aluminum-scandium (Al—Sc) alloy. A method can include providing an electrolyte bath comprising a first portion of at least one of ScF3 or AlF3 and a first portion of at least one of LiF, NaF, or KF; providing a cathode in electrical contact with the electrolyte bath; providing an anode in electrical contact with the electrolyte bath; adding a first portion of SC2O3 into the electrolyte bath; reacting an aluminum ion with the cathode; applying an electric current to the cathode, thereby reacting a scandium ion with the cathode to produce the Al—Sc alloy.

Abstract: The disclosure relates generally to molecular diagnostic devices configured to amplifying a single nucleotide polymorphism (SNP) locus and discriminate between two or more allelic variants of the SNP, indicating presence or absence of a target allele. In some embodiments, the molecular diagnostic devices are capable of detecting, at point-of-care, SNPs associated with resistance or susceptibility to antibiotic treatment of organism infections. In other aspects, the disclosure provides methods of treatment for disease or disorders (e.g. organism infections) where treatment is guided by presence or absence of an allele at a SNP locus as determined by such molecular diagnostic devices.

Abstract: Methods and compositions are provided herein for assessing CRISPR/Cas-mediated non-homologous end joining (NHEJ) activity and/or CRISPR/Cas-induced recombination of a target genomic locus with an exogenous donor nucleic acid in vivo and ex vivo. The methods and compositions employ cells and non-human animals comprising a Cas expression cassette such as a genomically integrated Cas expression cassette so that the Cas protein can be constitutively available or available in a tissue-specific or temporal-specific manner. Methods and compositions are also provided for making and using these non-human animals, including use of these non-human animals to assess CRISPR/Cas activity in vivo via adeno-associated virus (AAV)-mediated delivery of guide RNAs to the non-human animals.

Abstract: A manipulator may include a base portion, an upright portion connected to the base portion, a test head attachment incorporated into the upright portion, and an upright quick connect assembly incorporated into the test head attachment. In some cases, the manipulator includes an automatic weight transfer mechanism, a mobile base portion with casters and/or steerable casters.

Abstract: A manipulator may include a base portion, an upright portion connected to the base portion, a test head attachment incorporated into the upright portion, and an upright quick connect assembly incorporated into the test head attachment. In some cases, the manipulator includes an automatic weight transfer mechanism, a mobile base portion with casters and/or steerable casters.

Abstract: A manipulator may include a base portion, an upright portion connected to the base portion, a test head attachment incorporated into the upright portion, and an upright quick connect assembly incorporated into the test head attachment. In some cases, the manipulator includes an automatic weight transfer mechanism, a mobile base portion with casters and/or steerable casters.

Abstract: A dual printed circuit board assembly for vehicle lights, the dual printed circuit board including a dual printed circuit board and a dual connector. The dual printed circuit board having a first substrate with a first layout, a second substrate opposite to the first substrate with a second layout, and a common panel that supports the first substrate on a first side and the second substrate on a second side, wherein the first layout is populable by a first plurality of light sources to be used in a first light of the vehicle lights and the second layout is populable by a second plurality of light sources to be used in a second light of the vehicle lights. The dual connector having a first jumper connector electrically connected to the first layout, and a second jumper connector opposite to the first jumper connector and electrically connected to the second layout.

Abstract: A light assembly to ground electronic elements, including a platform that supports a front part of the electronic elements; a housing that supports the electronic elements and the platform, the housing having an anchor that protrudes from the housing to support the platform, a plurality of ridges that protrudes from the housing to support a back part of the electronic elements, a back and a front reflective layers that cover the plurality of ridges and the anchor; and a connector that provides electrical connections between each electronic elements. The front and back reflective layers provide grounding for the electronic elements via the plurality of ridges and the anchor, the connector provides electrical connections between the electronic elements and the back reflective layer, and the platform support provides retaining of the electrical connections provided by the connector.

Abstract: A dual printed circuit board assembly for vehicle lights, the dual printed circuit board including a dual printed circuit board and a dual connector. The dual printed circuit board having a first substrate with a first layout, a second substrate opposite to the first substrate with a second layout, and a common panel that supports the first substrate on a first side and the second substrate on a second side, wherein the first layout is populable by a first plurality of light sources to be used in a first light of the vehicle lights and the second layout is populable by a second plurality of light sources to be used in a second light of the vehicle lights. The dual connector having a first jumper connector electrically connected to the first layout, and a second jumper connector opposite to the first jumper connector and electrically connected to the second layout.

Abstract: A light assembly to ground electronic elements, including a platform that supports a front part of the electronic elements; a housing that supports the electronic elements and the platform, the housing having an anchor that protrudes from the housing to support the platform, a plurality of ridges that protrudes from the housing to support a back part of the electronic elements, a back and a front reflective layers that cover the plurality of ridges and the anchor; and a connector that provides electrical connections between each electronic elements. The front and back reflective layers provide grounding for the electronic elements via the plurality of ridges and the anchor, the connector provides electrical connections between the electronic elements and the back reflective layer, and the platform support provides retaining of the electrical connections provided by the connector.

Abstract: Described herein is a connector, an electric circuit and a lighting apparatus. The connector includes a connector body having a top edge, a bottom edge and a wall connecting the top edge and the bottom edge, the wall providing flexibility to move the top edge relative to the bottom edge, a plurality of metal contacts embedded within the top edge and the bottom edge, locking teeth projecting from the top edge and the bottom edge, wherein the top edge and the bottom edge move away from each other upon application of a force on the locking teeth. Furthermore, the connector can be connector to a printed circuit board. The printed circuit board includes a plurality of pockets configured to receive the locking teeth of the connector and a plurality of metal strips configured to contact with the plurality of the metal contacts of the connector.

Abstract: A manipulator may include a base portion, an upright portion connected to the base portion, a test head attachment incorporated into the upright portion, and an upright quick connect assembly incorporated into the test head attachment. In some cases, the manipulator includes an automatic weight transfer mechanism, a mobile base portion with casters and/or steerable casters.

Abstract: A smoke removal apparatus, capable of operating in a confined space without ventilation of the apparatus to the atmosphere to provide removal of smoke from air to be treated. The apparatus includes one or more of the following filters: a mechanical filter; an electrostatic filter; and/or a chemical filter. The apparatus is arranged to collect smoke from a relatively higher position, pass smoke through the one or more filters/and expel treated air to a relatively lower position.

Abstract: A vertical device geometry for a carbon-nanotube-based field effect transistor has one or multiple carbon nanotubes formed in a trench.

Abstract: A vertical device geometry for a carbon-nanotube-based field effect transistor has one or multiple carbon nanotubes formed in a trench.

Abstract: A property investment management tool comprises user interface logic and an investment rating engine. The user interface logic is configured to provide a user interface accessible to a user and includes risk profile configuration interface logic configured to permit a user to define a plurality of different risk profiles for a plurality of different investments. The investment rating engine is coupled to the user interface logic and is configured to evaluate the plurality of different investments according to the plurality of different risk profiles.

Abstract: A multifamily investment management tool comprises user interface logic and an investment rating engine. The user interface logic is configured to provide a user interface accessible to a user and includes risk profile configuration interface logic configured to permit a user to define a plurality of different risk profiles for a plurality of different investments. The investment rating engine is coupled to the user interface logic and is configured to evaluate the plurality of different investments according to the plurality of different risk profiles.

Abstract: A smoke removal apparatus, capable of operating in a confined space without ventilation of the apparatus to the atmosphere to provide removal of smoke from air to be treated. The apparatus comprises one or more of the following filters: a mechanical filter (2); an electrostatic filter (3); and/or a chemical filter (4). The apparatus is arranged to collect smoke from a relatively higher position (5), pass smoke through the one or more filters/and expel treated air to a relatively lower position (6).

Abstract: A vertical device geometry for a carbon-nanotube-based field effect transistor has one or multiple carbon nanotubes formed in a trench.