Abstract: An assay device includes a colorimetric testing assembly including a detection area, a fluid inlet, and a microfluidic network including a first path extending to the detection area and a second path extending to the detection area. When a fluid (e.g., a buffer fluid or a combined buffer and sample solution) is provided to the fluid inlet, a first portion of the fluid rehydrates a first dried reagent (e.g., a dried enzyme label) disposed along the first path to produce a first rehydrated reagent and a second portion of the fluid rehydrates a second dried reagent (e.g., a dried substrate) to produce a second rehydrated reagent. The first rehydrated reagent and the second rehydrated reagent are then sequentially delivered to the detection area by capillary-driven flow to perform the assay.

Abstract: An assay device includes an electrochemical testing assembly having a test channel including a capture reagent selected to capture a target analyte and an electrode having a surface in communication with the test channel. The assay device further includes a microfluidic network in communication with the test channel, a buffer fluid inlet in communication with the microfluidic network, and a detection reagent disposed within the microfluidic network. When a buffer fluid is provided to the buffer fluid inlet, the buffer fluid transports the detection reagent to the test channel by capillary-driven flow, and wherein the electrode is configured to measure an electrical response indicating capture of the target analyte by the capture reagent after transportation of the detection reagent to the test channel.

Abstract: The invention discloses a method and a system to detect a target nucleic acid sequence in a sample using padlock probe-based rolling circle amplification and nuclease protection. Padlock probe-based rolling circle amplification and nuclease protection may be used in combination with other detection assays to detect target nucleic acid sequences in a sample.

Abstract: Antimicrobial resistance (AMR), the ability of a bacterial species to resist the action of an antimicrobial drug, has been on the rise due to the widespread use of antimicrobial agents, and one of the many ways AMR can spread is through contaminated water sources. To monitor these water sources, we have developed an inexpensive, fast assay using a paper-based analytical device (PAD) that can test for the presence of ?-lactamase-mediated resistance as one major form of AMR that has reliably detected resistance in sewage water.

Abstract: The invention provides methods to inhibit the replication of a flavivirus, methods of inhibiting the guanosine triphosphate (GTP)-binding and guanylyltransferase activity of a flavivirus RNA capping enzyme, and methods of treating a subject infected with a flavivirus. The methods can include contacting a flavivirus with an effective amount of a thioxothiazolidine compound described herein, or a derivative thereof, such as a compound of Formula (I).

Abstract: The invention provides methods to inhibit the replication of a flavivirus, methods of inhibiting the guanosine triphosphate (GTP)-binding and guanylyltransferase activity of a flavivirus RNA capping enzyme, and methods of treating a subject infected with a flavivirus. The methods can include contacting a flavivirus with an effective amount of a thioxothiazolidine compound described herein, or a derivative thereof, such as a compound of Formula (I).