Patents by Inventor Brian J. NANKERVIS
Brian J. NANKERVIS has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20250145929Abstract: Embodiments described herein generally provide for expanding cells in a cell expansion system. The cells may be grown in a bioreactor, and the cells may be activated by an activator (e.g., a soluble activator complex). Nutrient and gas exchange capabilities of a closed, automated cell expansion system may allow cells to be seeded at reduced cell seeding densities, for example. Parameters of the cell growth environment may be manipulated to load the cells into a particular position in the bioreactor for the efficient exchange of nutrients and gases. System parameters may be adjusted to shear any cell colonies that may form during the expansion phase. Metabolic concentrations may be controlled to improve cell growth and viability. Cell residence in the bioreactor may be controlled. In embodiments, the cells may include T cells. In further embodiments, the cells may include T cell subpopulations, including regulatory T cells (Tregs), helper, na“i”ve, memory, or effector, for example.Type: ApplicationFiled: January 13, 2025Publication date: May 8, 2025Inventors: Boah VANG, Brian J. NANKERVIS, Mark E. JONES
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Publication number: 20240060030Abstract: Embodiments described herein generally provide for expanding cells in a cell expansion system. The cells may be grown in a bioreactor, and the cells may be activated by an activator (e.g., a soluble activator complex). Nutrient and gas exchange capabilities of a closed, automated cell expansion system may allow cells to be seeded at reduced cell seeding densities, for example. Parameters of the cell growth environment may be manipulated to load the cells into a particular position in the bioreactor for the efficient exchange of nutrients and gases. System parameters may be adjusted to shear any cell colonies that may form during the expansion phase. Metabolic concentrations may be controlled to improve cell growth and viability. Cell residence in the bioreactor may be controlled. In embodiments, the cells may include T cells. In further embodiments, the cells may include T cell subpopulations, including regulatory T cells (Tregs), for example.Type: ApplicationFiled: August 11, 2023Publication date: February 22, 2024Inventors: Dalip SETHI, James ARTHUR, David Alan HANNA, Daniel CLEMENT, Brian J. NANKERVIS, Jerome HEINTZ, Nathan D. FRANK, Brett BAEVERSTAD, Dennis J. HLAVINKA
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Publication number: 20240018475Abstract: Embodiments described herein generally provide for expanding cells in a cell expansion system. The cells may be grown in a bioreactor, and the cells may be activated by an activator (e.g., a soluble activator complex). Nutrient and gas exchange capabilities of a closed, automated cell expansion system may allow cells to be seeded at reduced cell seeding densities, for example. Parameters of the cell growth environment may be manipulated to load the cells into a particular position in the bioreactor for the efficient exchange of nutrients and gases. System parameters may be adjusted to shear any cell colonies that may form during the expansion phase. Metabolic concentrations may be controlled to improve cell growth and viability. Cell residence in the bioreactor may be controlled. In embodiments, the cells may include T cells. In further embodiments, the cells may include T cell subpopulations, including regulatory T cells (Tregs), for example.Type: ApplicationFiled: September 20, 2023Publication date: January 18, 2024Inventors: Brian J. NANKERVIS, Domicinda M. HILL, Mark E. JONES
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Publication number: 20230340385Abstract: Embodiments described herein generally provide for expanding cells in a cell expansion system. The cells may be grown in a bioreactor, and the cells may be activated by an activator (e.g., a soluble activator complex). Nutrient and gas exchange capabilities of a closed, automated cell expansion system may allow cells to be seeded at reduced cell seeding densities, for example. Parameters of the cell growth environment may be manipulated to load the cells into a particular position in the bioreactor for the efficient exchange of nutrients and gases. System parameters may be adjusted to shear any cell colonies that may form during the expansion phase. Metabolic concentrations may be controlled to improve cell growth and viability. Cell residence in the bioreactor may be controlled. In embodiments, the cells may include T cells. In further embodiments, the cells may include T cell subpopulations, including regulatory T cells (Tregs), helper, naïve, memory, or effector, for example.Type: ApplicationFiled: February 3, 2023Publication date: October 26, 2023Applicant: Terumo BCT, Inc.Inventors: Brian J. NANKERVIS, Mark E. JONES, Domicinda M. HILL
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Publication number: 20230332105Abstract: Embodiments described herein generally provide for expanding cells in a cell expansion system. The cells may be grown in a bioreactor, and the cells may be activated by an activator (e.g., a soluble activator complex). Nutrient and gas exchange capabilities of a closed, automated cell expansion system may allow cells to be seeded at reduced cell seeding densities, for example. Parameters of the cell growth environment may be manipulated to load the cells into a particular position in the bioreactor for the efficient exchange of nutrients and gases. System parameters may be adjusted to shear any cell colonies that may form during the expansion phase. Metabolic concentrations may be controlled to improve cell growth and viability. Cell residence in the bioreactor may be controlled. In embodiments, the cells may include T cells. In further embodiments, the cells may include T cell subpopulations, including regulatory T cells (Tregs), for example.Type: ApplicationFiled: May 9, 2023Publication date: October 19, 2023Inventors: Brian J. NANKERVIS, Domicinda M. HILL, Mark E. JONES
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Publication number: 20230193180Abstract: Embodiments described herein generally provide for the expansion of cells in a cell expansion system using an active promotion of a coating agent(s) to a cell growth surface in some embodiments. A coating agent may be applied to a surface, such as the cell growth surface of a hollow fiber in a bioreactor, by controlling the movement of a fluid in which a coating agent is suspended, by changing flow rates, by changing flow directions, by rotation of the bioreactor, and/or combinations thereof.Type: ApplicationFiled: February 16, 2023Publication date: June 22, 2023Inventors: Nathan D. FRANK, Brian J. NANKERVIS, Dennis J. HLAVINKA, Thomas G. DILORENZO
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Publication number: 20230174917Abstract: Embodiments for loading and expanding particular cell types are described. Embodiments may include the use of hollow fiber membranes with particular characteristic such as hollow fibers with inner diameters that provide mechanical stimulus (e.g., radius of curvature greater than a dimension of a cell). In addition, embodiments may provide for manipulation of flow rates and other features that also provide mechanical stimuli and promote or enhance the growth of particular types of cells.Type: ApplicationFiled: January 30, 2023Publication date: June 8, 2023Inventors: Mark E. JONES, Brian J. NANKERVIS, Kristina E. FUERST, Jon A. DODD
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Publication number: 20210047602Abstract: Embodiments described herein generally provide for expanding cells in a cell expansion system. The cells may be grown in a bioreactor, and the cells may be activated by an activator (e.g., a soluble activator complex). Nutrient and gas exchange capabilities of a closed, automated cell expansion system may allow cells to be seeded at reduced cell seeding densities, for example. Parameters of the cell growth environment may be manipulated to load the cells into a particular position in the bioreactor for the efficient exchange of nutrients and gases. System parameters may be adjusted to shear any cell colonies that may form during the expansion phase. Metabolic concentrations may be controlled to improve cell growth and viability. Cell residence in the bioreactor may be controlled. In embodiments, the cells may include T cells. In further embodiments, the cells may include T cell subpopulations, including regulatory T cells (Tregs), helper, naïve, memory, or effector, for example.Type: ApplicationFiled: November 2, 2020Publication date: February 18, 2021Applicant: Terumo BCT, Inc.Inventors: Boah VANG, Brian J. NANKERVIS, Mark E. JONES
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Publication number: 20200255786Abstract: Described are embodiments for expanding cells in a bioreactor. In one embodiment, methods are provided that distribute cells throughout the bioreactor and attach cells to specific portions of a bioreactor to improve the expansion of the cells in the bioreactor. Embodiments may be implemented on a cell expansion system configured to load, distribute, attach and expand cells.Type: ApplicationFiled: April 27, 2020Publication date: August 13, 2020Applicant: Terumo BCT, Inc.Inventors: Brian J. NANKERVIS, Mark E. JONES
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Publication number: 20200248126Abstract: Described are embodiments for expanding cells in a bioreactor. In one embodiment, methods are provided that distribute cells throughout the bioreactor and attach cells to specific portions of a bioreactor to improve the expansion of the cells in the bioreactor. Embodiments may be implemented on a cell expansion system configured to load, distribute, attach and expand cells.Type: ApplicationFiled: April 24, 2020Publication date: August 6, 2020Applicant: Terumo BCT, Inc.Inventors: Brian J. NANKERVIS, Mark E. JONES
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Publication number: 20200239819Abstract: Embodiments described herein generally provide for the expansion of cells in a cell expansion system using an active promotion of a coating agent(s) to a cell growth surface. A coating agent may be applied to a surface, such as the cell growth surface of a hollow fiber, by controlling the movement of a fluid in which a coating agent is suspended. Using ultrafiltration, the fluid may be pushed through the pores of a hollow fiber from a first side, e.g., an intracapillary (IC) side, of the hollow fiber to a second side, e.g., an extracapillary (EC) side, while the coating agent is actively promoted to the surface of the hollow fiber. In so doing, the coating agent may be hydrostatically deposited onto a wall, e.g., inner wall, of the hollow fiber.Type: ApplicationFiled: April 10, 2020Publication date: July 30, 2020Applicant: Terumo BCT, Inc.Inventors: Nathan D. FRANK, Brian J. NANKERVIS, Dennis J. HLAVINKA, Thomas G. DILORENZO
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Publication number: 20200239822Abstract: Embodiments described herein generally provide for the expansion of cells in a cell expansion system using an active promotion of a coating agent(s) to a cell growth surface in some embodiments. A coating agent may be applied to a surface, such as the cell growth surface of a hollow fiber in a bioreactor, by controlling the movement of a fluid in which a coating agent is suspended, by changing flow rates, by changing flow directions, by rotation of the bioreactor, and/or combinations thereof.Type: ApplicationFiled: April 10, 2020Publication date: July 30, 2020Applicant: Terumo BCT, Inc.Inventors: Nathan D. FRANK, Brian J. NANKERVIS, Dennis J. HLAVINKA, Thomas G. DILORENZO
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Publication number: 20190382709Abstract: Embodiments described herein provide for the production, isolation, and/or collection of cellular product(s) released or secreted from cells. Cells may be expanded in the intracapillary (or extracapillary) space of a bioreactor of a cell expansion system with media. Cells may release cellular products into the fluid space of the bioreactor. Examples of such released cellular products include extracellular particles, such as extracellular vesicles (EVs). To collect the extracellular particles released from the cells being expanded, as opposed to any extracellular particles from other sources, a washout procedure may be used to eliminate any serum proteins prior to collecting the released extracellular particles from the expanding cells. The released cellular products may be collected or concentrated through the control of outlet parameters, while nutrients may reach the cells through the diffusion of media through a semi-permeable membrane, for example. The released cellular products may then be harvested.Type: ApplicationFiled: May 5, 2017Publication date: December 19, 2019Applicant: Terumo BCT, Inc.Inventors: Boah VANG, Brian J. NANKERVIS
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Publication number: 20190062683Abstract: Embodiments described herein generally provide for expanding cells in a cell expansion system. The cells may be grown in a bioreactor, and the cells may be activated by an activator (e.g., a soluble activator complex). Nutrient and gas exchange capabilities of a closed, automated cell expansion system may allow cells to be seeded at reduced cell seeding densities, for example. Parameters of the cell growth environment may be manipulated to load the cells into a particular position in the bioreactor for the efficient exchange of nutrients and gases. System parameters may be adjusted to shear any cell colonies that may form during the expansion phase. Metabolic concentrations may be controlled to improve cell growth and viability. Cell residence in the bioreactor may be controlled. In embodiments, the cells may include T cells. In further embodiments, the cells may include T cell subpopulations, including regulatory T cells (Tregs), helper, naïve, memory, or effector, for example.Type: ApplicationFiled: August 24, 2018Publication date: February 28, 2019Applicant: Terumo BCT, Inc.Inventors: Brian J. NANKERVIS, Mark E. JONES, Domicinda M. HILL
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Publication number: 20180291342Abstract: Embodiments described herein generally provide for expanding cells in a cell expansion system. The cells may be grown in a bioreactor, and the cells may be activated by an activator (e.g., a soluble activator complex). Nutrient and gas exchange capabilities of a closed, automated cell expansion system may allow cells to be seeded at reduced cell seeding densities, for example. Parameters of the cell growth environment may be manipulated to load the cells into a particular position in the bioreactor for the efficient exchange of nutrients and gases. System parameters may be adjusted to shear any cell colonies that may form during the expansion phase. Metabolic concentrations may be controlled to improve cell growth and viability. Cell residence in the bioreactor may be controlled. In embodiments, the cells may include T cells. In further embodiments, the cells may include T cell subpopulations, including regulatory T cells (Tregs), for example.Type: ApplicationFiled: April 2, 2018Publication date: October 11, 2018Applicant: Terumo BCT, Inc.Inventors: Mark E. JONES, Brian J. NANKERVIS
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Publication number: 20180282695Abstract: Embodiments described herein generally provide for expanding cells in a cell expansion system. The cells may be grown in a bioreactor, and the cells may be activated by an activator (e.g., a soluble activator complex). Nutrient and gas exchange capabilities of a closed, automated cell expansion system may allow cells to be seeded at reduced cell seeding densities, for example. Parameters of the cell growth environment may be manipulated to load the cells into a particular position in the bioreactor for the efficient exchange of nutrients and gases. System parameters may be adjusted to shear any cell colonies that may form during the expansion phase. Metabolic concentrations may be controlled to improve cell growth and viability. Cell residence in the bioreactor may be controlled. In embodiments, the cells may include T cells. In further embodiments, the cells may include T cell subpopulations, including regulatory T cells (Tregs), for example.Type: ApplicationFiled: April 2, 2018Publication date: October 4, 2018Applicant: Terumo BCT, Inc.Inventors: Brian J. NANKERVIS, Domicinda M. HILL, Mark E. JONES
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Publication number: 20180142199Abstract: Embodiments for loading and expanding particular cell types are described. Embodiments may include the use of hollow fiber membranes with particular characteristic such as hollow fibers with inner diameters that provide mechanical stimulus (e.g., radius of curvature greater than a dimension of a cell). In addition, embodiments may provide for manipulation of flow rates and other features that also provide mechanical stimuli and promote or enhance the growth of particular types of cells.Type: ApplicationFiled: December 22, 2017Publication date: May 24, 2018Applicant: Terumo BCT, Inc.Inventors: Mark E. JONES, Brian J. NANKERVIS, Kristina E. FUERST, Jon A. DODD
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Publication number: 20180119094Abstract: Embodiments described herein generally relate to passively replacing media in a closed cell expansion system to reduce or prevent the dilution of chemical signaling used to inhibit signaling pathways that keep a cell population in the lag phase of cell growth. To prevent such dilution, active inlet fluid flow to the system may be halted. To replace fluid lost by the system, a bag containing media may be attached to the waste line in replacement of the waste or outlet bag connected thereto. By turning off one or more pumps, media from the replacement bag is added to the system at the rate of evaporation. Chemical signaling dilution may be prevented while conserving system resources. Enhancement of chemical signaling to reduce the lag phase of cell growth may further be accomplished by adding molecules, such as chemical-signaling proteins, from a direct source to the system.Type: ApplicationFiled: December 20, 2017Publication date: May 3, 2018Applicant: Terumo BCT, Inc.Inventor: Brian J. NANKERVIS
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Publication number: 20170349869Abstract: Embodiments described herein generally provide for the expansion of cells in a cell expansion system using an active promotion of a coating agent(s) to a cell growth surface. A coating agent may be applied to a surface, such as the cell growth surface of a hollow fiber, by controlling the movement of a fluid in which a coating agent is suspended. Using ultrafiltration, the fluid may be pushed through the pores of a hollow fiber from a first side, e.g., an intracapillary (IC) side, of the hollow fiber to a second side, e.g., an extracapillary (EC) side, while the coating agent is actively promoted to the surface of the hollow fiber. In so doing, the coating agent may be hydrostatically deposited onto a wall, e.g., inner wall, of the hollow fiber.Type: ApplicationFiled: June 7, 2017Publication date: December 7, 2017Applicant: Terumo BCT, Inc.Inventors: Nathan D. FRANK, Brian J. NANKERVIS, Dennis J. HLAVINKA, Thomas G. DILORENZO
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Publication number: 20170349873Abstract: Embodiments described herein generally provide for the expansion of cells in a cell expansion system using an active promotion of a coating agent(s) to a cell growth surface in some embodiments. A coating agent may be applied to a surface, such as the cell growth surface of a hollow fiber in a bioreactor, by controlling the movement of a fluid in which a coating agent is suspended, by changing flow rates, by changing flow directions, by rotation of the bioreactor, and/or combinations thereof.Type: ApplicationFiled: June 7, 2017Publication date: December 7, 2017Applicant: Terumo BCT, Inc.Inventors: Nathan D. FRANK, Brian J. NANKERVIS, Dennis J. HLAVINKA, Thomas G. DILORENZO