Abstract: The present invention provides for methods of DNA methylation detection. The present invention provides for methods of generating and detecting specific electronic signals that report the methylation status of targeted DNA molecules in biological samples. Two methods are described, direct and indirect detection of methylated DNA molecules in a nano transistor based device. In the direct detection, methylated target DNA molecules are captured on the sensing surface resulting in changes in the electrical properties of a nano transistor. These changes generate detectable electronic signals. In the indirect detection, antibody-DNA conjugates are used to identify methylated DNA molecules. RNA signal molecules are generated through an in vitro transcription process. These RNA molecules are captured on the sensing surface change the electrical properties of nano transistor thereby generating detectable electronic signals.

Abstract: The present invention provides for methods of DNA methylation detection. The present invention provides for methods of generating and detecting specific electronic signals that report the methylation status of targeted DNA molecules in biological samples. Two methods are described, direct and indirect detection of methylated DNA molecules in a nano transistor based device. In the direct detection, methylated target DNA molecules are captured on the sensing surface resulting in changes in the electrical properties of a nano transistor. These changes generate detectable electronic signals. In the indirect detection, antibody-DNA conjugates are used to identify methylated DNA molecules. RNA signal molecules are generated through an in vitro transcription process. These RNA molecules are captured on the sensing surface change the electrical properties of nano transistor thereby generating detectable electronic signals.

Abstract: The present invention provides methods for the detection of bioluminescence resonance energy transfer. The present invention provides methods of generating a report element and its applications in high throughput detection of bio-agents and gene expression levels. The present invention also relates to a nucleic acid signal amplification process. The present invention provides a report element where a fluorescent dye is directly conjugated to redox enzyme for use in bioluminescence resonant energy transfer (BRET) assays. The present invention also provides a pair of highly homogeneous luciferases for a dual BRET report system allowing the comparison of gene expression levels. With this report element, charge coupled device (CCD) and CMOS based optical detection mechanisms may be used for highly sensitive, high throughput and low cost portable luminescence biosensors.