Abstract: The invention provides methods for detection and typing of HPV infection using PNA probes. More specifically, methods are provided for detecting high-risk types of HPV infection with minimal numbers of PNA probes or using PNA probes to selectively amplify only high-risk types of HPV.

Abstract: Disclosed are methods and compositions for extracting nucleic acids from a biological sample. In particular, disclosed is a nucleic acid extraction solution together with methods using such a solution for extracting nucleic acid sequences from biological samples containing cells, cellular debris or both. The nucleic acid extraction solution contains a molecule having the formula R1O—CH2—CH2—OR2, wherein R1 and R2 independently are selected from the group consisting of hydrogen and an alkyl group.

Abstract: A method for analyzing a sample to detect cells infected by Human Papilloma Virus (HPV) is provided. The method includes passing a medium containing the sample across a filter. The filter has a pore size that is greater than a dimension of a HPV particle, but smaller than a dimension of a HPV infected cell, such that most of the HPV particles pass through the filter, while leaving the cells on the filter. The material collected on the filter is then examined to determine if HPV infected cells are present in the material.

Abstract: The invention provides methods for detection and typing of HPV infection using PNA probes.

Abstract: A method is provided for the simultaneous detection of human papillomavirus DNA and RNA in a sample.

Abstract: Disclosed are methods and compositions for extracting nucleic acids from a biological sample. In particular, disclosed is a nucleic acid extraction solution together with methods using such a solution for extracting nucleic acid sequences from biological samples containing cells, cellular debris or both. The nucleic acid extraction solution contains a molecule having the formula R1O—CH2—CH2—OR2, wherein R1 and R2 independently are selected from the group consisting of hydrogen and an alkyl group.

Abstract: The invention provides materials and methods for detection and typing of HPV infection using PNA probes. More specifically, methods are provided for detecting high-risk types of HPV infection with minimal numbers of PNA probes or using PNA probes to selectively amplify only high-risk types of HPV. Novel primer sequences are also provided.

Abstract: The invention provides a method for the assessment of biological markers in a sample of tissue, cells or fluid for the detection a malignant or pre-malignant condition. Aspects of the present invention are particularly useful in screening samples such as cervical smears from women to detect such markers. Identification of a malignant or pre-malignant condition may be followed by appropriate treatment.

Abstract: A method for analyzing a sample to detect cells infected by Human Papilloma Virus (HPV) is provided. The method includes passing a medium containing the sample across a filter. The filter has a pore size that is greater than a dimension of a HPV particle, but smaller than a dimension of a HPV infected cell, such that most of the HPV particles pass through the filter, while leaving the cells on the filter. The material collected on the filter is then examined to determine if HPV infected cells are present in the material.

Abstract: A method for assaying a sample for the presence of a target is provided. The method comprises drawing a liquid sample transversely through a filter using an automated device that can control the rate of flow through the filter. A sensor molecule is attached to the filter which can bind to a target molecule. Binding of the target to the attached sensor molecule can then be detected through any of various techniques. The methods may be performed in multiplex form to permit simultaneous analysis of a plurality of targets. Kits for performing the methods are also provided.

Abstract: The invention provides materials and methods for detection and typing of HPV infection using PNA probes. More specifically, methods are provided for detecting high-risk types of HPV infection with minimal numbers of PNA probes or using PNA probes to selectively amplify only high-risk types of HPV. Novel primer sequences are also provided.

Abstract: The invention provides methods for detection and typing of HPV infection using PNA probes. More specifically, methods are provided for detecting high-risk types of HPV infection with minimal numbers of PNA probes or using PNA probes to selectively amplify only high-risk types of HPV.

Abstract: The invention provides methods for detection and typing of HPV infection using PNA probes. More specifically, methods are provided for detecting high-risk types of HPV infection with minimal numbers of PNA probes or using PNA probes to selectively amplify only high-risk types of HPV.

Abstract: Disclosed are methods and compositions for extracting nucleic acids from a biological sample. In particular, disclosed is a nucleic acid extraction solution together with methods using such a solution for extracting nucleic acid sequences from biological samples containing cells, cellular debris or both. The nucleic acid extraction solution contains a molecule having the formula R1O—CH2—CH2—OR2, wherein R1 and R2 independently are selected from the group consisting of hydrogen and an alkyl group.