Abstract: A power take off unit includes an input gear, an output gear, and an intermediary gear that cooperate to transfer power from a rotational power source to an operating target. The power take off unit has a control module that biases the intermediary gear relative to the input gear and the output gear to reduce gear rattle vibrations associated with intermeshed tooth looseness.

Abstract: A sealed and lubricated rotatable track engaging element includes a roller and a roller shaft extending through the roller to support the roller for rotation about a major axis. A first thrust surface is fixed relative to the roller, and a second thrust surface is fixed relative to the roller shaft. A seal cavity extends between the roller and the roller shaft and is formed in part by each of the first thrust surface and the second thrust surface, and a thrust washer stack is within the seal cavity and trapped between the first thrust surface and the second thrust surface. The thrust washer stack includes an inner thrust washer contacting the roller to rotate at a first fractional speed of a speed of rotation of the roller, and an outer thrust washer contacting the inner thrust washer and the collar to rotate at a second fractional speed that is less than the first fractional speed.

Abstract: A sealed and lubricated rotatable track engaging element includes a roller and a roller shaft extending through the roller to support the roller for rotation about a major axis. A first thrust surface is fixed relative to the roller, and a second thrust surface is fixed relative to the roller shaft. A seal cavity extends between the roller and the roller shaft and is formed in part by each of the first thrust surface and the second thrust surface, and a thrust washer stack is within the seal cavity and trapped between the first thrust surface and the second thrust surface. The thrust washer stack includes an inner thrust washer contacting the roller to rotate at a first fractional speed of a speed of rotation of the roller, and an outer thrust washer contacting the inner thrust washer and the collar to rotate at a second fractional speed that is less than the first fractional speed.

Abstract: A system and a computer-based method for providing bundled services to a client application in a service call to a service system in a service provider computer system includes receiving a message defining an API service request comprising at least a parameter portion and a payload portion, determining at the gateway system an identity of an application transmitting the received message using identity information that has been established within the service provider computer system, providing, by a services platform, at least one of encryption services and decryption services for data contained in the payload portion using the parameters received in the parameter portion, managing key material for security of the data, and transmitting the encrypted data back to the calling application.

Abstract: The invention provides methods of detecting a nucleic acid present in a biological sample, comprising combining the biological sample with a lysis buffer to form a lysis mixture comprising nucleic acid released from cells in said biological sample; and subjecting a volume of the lysis mixture to size-exclusion chromatography in a column comprising a volume of size-exclusion medium. In certain embodiments, the lysis buffer separates double-stranded nucleic acid into single-stranded nucleic acid. In certain embodiments, the elution can have a flow rate of separation of less than 10 minutes to produce an eluted solution comprising isolated nucleic acid. The invention provides for a method of accurately and rapidly detecting products of nucleic acid amplification.

Abstract: Networks and methods for use in authenticating messages, based on the clients and the computing devices, are provided. One exemplary method generally includes performing, by an API gateway, validation of a computing device based on a certificate identifying the computing device as one of the recognized computing devices, via the repository, and performing, by the API gateway, validation of the client based on the client certificate via a global access manager, separate from the repository. The exemplary method further includes causing a security token indicative of the client to be generated, when the computing device and the client are validated, whereby the security token is indicative of the client and permits the message, from the client, to be delivered to one or more backend services.

Abstract: The invention provides methods of detecting a nucleic acid present in a biological sample, comprising combining the biological sample with a lysis buffer to form a lysis mixture comprising nucleic acid released from cells in said biological sample; and subjecting a volume of the lysis mixture to size-exclusion chromatography in a column comprising a volume of size-exclusion medium. In certain embodiments, the lysis buffer separates double-stranded nucleic acid into single-stranded nucleic acid. In certain embodiments, the elution can have a flow rate of separation of less than 10 minutes to produce an eluted solution comprising isolated nucleic acid. The invention provides for a method of accurately and rapidly detecting products of nucleic acid amplification.

Abstract: Networks and methods for use in authenticating messages, based on the clients and the computing devices, are provided. One exemplary method generally includes performing, by an API gateway, validation of a computing device based on a certificate identifying the computing device as one of the recognized computing devices, via the repository, and performing, by the API gateway, validation of the client based on the client certificate via a global access manager, separate from the repository. The exemplary method further includes causing a security token indicative of the client to be generated, when the computing device and the client are validated, whereby the security token is indicative of the client and permits the message, from the client, to be delivered to one or more backend services.

Abstract: The invention provides methods of detecting a nucleic acid present in a biological sample, comprising combining the biological sample with a lysis buffer to form a lysis mixture comprising nucleic acid released from cells in said biological sample; and subjecting a volume of the lysis mixture to size-exclusion chromatography in a column comprising a volume of size-exclusion medium. In certain embodiments, the lysis buffer separates double-stranded nucleic acid into single-stranded nucleic acid. In certain embodiments, the elution can have a flow rate of separation of less than 10 minutes to produce an eluted solution comprising isolated nucleic acid. The invention provides for a method of accurately and rapidly detecting products of nucleic acid amplification.

Abstract: A system and a computer-based method for providing bundled services to a client application in a service call to a service system in a service provider computer system includes receiving a message defining an API service request comprising at least a parameter portion and a payload portion, determining at the gateway system an identity of an application transmitting the received message using identity information that has been established within the service provider computer system, providing, by a services platform, at least one of encryption services and decryption services for data contained in the payload portion using the parameters received in the parameter portion, managing key material for security of the data, and transmitting the encrypted data back to the calling application.