Abstract: In some examples, a method of coupling oligonucleotides to a polymer is provided. Inactive moieties in a first region of a polymer may be selectively irradiated with light, while inactive moieties in a second region of the polymer are not irradiated, to generate first active moieties in the first region of the polymer. The first active moieties may be coupled to first oligonucleotides. The inactive moieties in the second region of the polymer may be irradiated with light to generate second active moieties in the second region of the polymer. The second active moieties may be coupled to second oligonucleotides.

Abstract: The present disclosure relates, in general, to methods for improving preparation of a spatial transcriptomics RNA, library, for example a mRNA library, by improving capture of RNA transcript information from a tissue sample in situ. The spatial transcriptomics library from a tissue sample is useful to determine a genetic profile and help diagnose a person who has or is at risk of having a disease, such as cancer, genetic disease, autoimmune disease, and other indications, and improve treatment of the subject.

Abstract: In one example, a method of preparing a fluidic channel includes covalently coupling a first region of a substrate to a first region of a cover using first moieties covalently coupled to the first region of the substrate and second moieties covalently coupled to the first region of the cover. The covalent coupling between the first region of the substrate and the first region of the cover suspends a second region of the cover over a second region of the substrate to form a fluidic channel.

Abstract: An RNA sequencing library preparation process that that utilized transposition with or with a template switch oligonucleotide to generate the libraries having UMIs and spatial barcode information, and methods for improving RNA library preparation from tissue samples using template switching and thermal amplification to improve RNA library quality.

Abstract: In some examples, a structure is contacted with polynucleotides having a variety of lengths and each including first and second adapters. The structure includes a substrate including first and second regions spaced apart from one another by a gap of at least 100 nm, a first set of capture primers coupled to the first region of the substrate, and a second set of capture primers coupled to the second region of the substrate. The first adapter of the polynucleotide is hybridized to a capture primer of the first set of capture primers. Based on that polynucleotide being sufficiently long to bridge the gap, it is amplified using the first and second sets of capture primers. Based upon that polynucleotide being insufficiently long to bridge the gap, it is not amplified. Optionally, a wall may be disposed in the gap.

Abstract: In some examples, a method includes disposing a first hydrogel within a first recess of a substrate and over a pillar. The substrate may include a second recess in which the pillar is disposed, and a wall separating the first recess from the second recess. While the first hydrogel is disposed within the first recess, the pillar may be removed. After removing the pillar, a second hydrogel may be disposed within the second recess. Also provided herein are nonlimiting examples of manners in which recesses, walls, and patterned hydrogels may be formed.

Abstract: Reusable flow cells for sequencing which exhibit signal intensity retention over numerous use cycles, the active surface of which contains reactive sulfur moieties for reversible primer binding, methods of using such flow cells, reagents therefor, and kits containing the same.

Abstract: Some examples herein provide a hydrogel on a substrate. The hydrogel includes a three-dimensional network of polymer chains; first functional groups coupled to the polymer chains; amplification primers coupled to the polymer chains via the first functional groups; and second functional groups coupled to the polymer chains and reversibly cross-linking the polymer chains to one another. Some examples herein provide a method of using a hydrogel. The method includes hybridizing a target polynucleotide to an amplification primer coupled to a hydrogel; cleaving cross-linkages within the hydrogel within which the target polynucleotide is hybridized to the amplification primer; and amplifying the target polynucleotide using additional amplification primers within the hydrogel within which the cross-linkages have been cleaved.

Abstract: In some examples, novel photochemically-reversible hydrogels and nanogel particles are described comprising copolymer chains including at least one reactive alkene or reactive 1,4-diene end group capable of [2+2] or [2+2+2+2] photodimerization, respectively, at wavelengths >270 nm. In various examples, the photochemically-reversible hydrogels comprise copolymer chains including at least one —N3, —C?CH or —CO2H end group for dual functionality and/or pH responsiveness. For nucleic acid sequencing, amplification primers are grafted to photochemically-reversible hydrogels or nanogel particles reversibly bound to surfaces within a flow cell. After sequencing is complete, the photochemically-reversible hydrogel or nanogel particles is/are removable from the flow cell surfaces by irradiation, enabling the flow cell to be reusable.

Abstract: A method may be implemented for in-tip flow-through magnetic bead processing. A biological solution may include a plurality of magnetic beads suspended therein. The biological solution may be introduced to a tube via an opening in a tip portion of the tube. The tip portion of the tube may include a magnetizable material arranged in a flow path of the biological solution. The magnetizable material may include a ferromagnetic matrix or a wire within the tip portion. A magnetic field may be applied proximate to the tip portion of the tube using an electromagnetic coil. The electromagnetic coil may be wound around the tip portion. The biological solution may be removed from the tube, for example, via the opening in the tip portion. The plurality of magnetic beads may be captured within the magnetizable material in the tip portion using the magnetic field.

Abstract: In one example, a method of preparing a fluidic channel includes covalently coupling a first region of a substrate to a first region of a cover using first moieties covalently coupled to the first region of the substrate and second moieties covalently coupled to the first region of the cover. The covalent coupling between the first region of the substrate and the first region of the cover suspends a second region of the cover over a second region of the substrate to form a fluidic channel.

Abstract: In some examples, a method of coupling oligonucleotides to a polymer is provided. Inactive moieties in a first region of a polymer may be selectively irradiated with light, while inactive moieties in a second region of the polymer are not irradiated, to generate first active moieties in the first region of the polymer. The first active moieties may be coupled to first oligonucleotides. The inactive moieties in the second region of the polymer may be irradiated with light to generate second active moieties in the second region of the polymer. The second active moieties may be coupled to second oligonucleotides.

Abstract: Control method and apparatus for a manual spray gun includes display that is disposed on the spray gun and that provides information to an operator about one or more coating operation parameters. An auxiliary trigger may be used that enables an operator to makes selections of one or more coating operation parameters. The display and auxiliary trigger together permit an operator to make selections without having to divert attention or field of view away from the spray gun or the coating area, especially during a coating operation.

Abstract: Control method and apparatus for a manual spray gun includes display that is disposed on the spray gun and that provides information to an operator about one or more coating operation parameters. An auxiliary trigger may be used that enables an operator to makes selections of one or more coating operation parameters. The display and auxiliary trigger together permit an operator to make selections without having to divert attention or field of view away from the spray gun or the coating area, especially during a coating operation.

Abstract: Control method and apparatus for a manual spray gun may include a second manually actuated trigger disposed on the spray gun handle, with the second trigger being operational to select one or more coating operation parameters.

Abstract: Control method and apparatus for a manual spray gun includes display that is disposed on the spray gun and that provides information to an operator about one or more coating operation parameters. An auxiliary trigger may be used that enables an operator to makes selections of one or more coating operation parameters. The display and auxiliary trigger together permit an operator to make selections without having to divert attention or field of view away from the spray gun or the coating area, especially during a coating operation.

Abstract: Control method and apparatus for a manual spray gun may include a second manually actuated trigger disposed on the spray gun handle, with the second trigger being operational to select one or more coating operation parameters.

Abstract: Purge method and apparatus for a manual spray gun or coating material application device, in which purge air is introduced into the device through a handgrip portion that is manually held during a coating operation. Purge air first enters the coating material flow path after the purge air enters the handgrip portion.

Abstract: Control method and apparatus for a manual spray gun may include a second manually actuated trigger disposed on the spray gun handle, with the second trigger being operational to select one or more coating operation parameters.

Abstract: Purge method and apparatus for a manual spray gun or coating material application device, in which purge air is introduced into the device through a handgrip portion that is manually held during a coating operation. Purge air first enters the coating material flow path after the purge air enters the handgrip portion.