Patents by Inventor Brian McKeown
Brian McKeown has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11859247Abstract: The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.Type: GrantFiled: June 23, 2021Date of Patent: January 2, 2024Assignee: Oxford Nanopore Technologies PLCInventors: Lakmal Jayasinghe, John Hagan Pryce Bayley, Stephen Cheley, Brian McKeown, James White, James Clarke
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Publication number: 20230065890Abstract: The invention relates to adaptors for sequencing nucleic acids. The adaptors may be used to generate single stranded constructs of nucleic acid for sequencing purposes. Such constructs may contain both strands from a double stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) template. The invention also relates to the constructs generated using the adaptors, methods of making the adaptors and constructs, as well as methods of sequencing double stranded nucleic acids.Type: ApplicationFiled: August 24, 2022Publication date: March 2, 2023Applicant: Oxford Nanopore Technologies PLCInventor: Brian McKeown
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Patent number: 11459606Abstract: The invention relates to adaptors for sequencing nucleic acids. The adaptors may be used to generate single stranded constructs of nucleic acid for sequencing purposes. Such constructs may contain both strands from a double stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) template. The invention also relates to the constructs generated using the adaptors, methods of making the adaptors and constructs, as well as methods of sequencing double stranded nucleic acids.Type: GrantFiled: February 27, 2018Date of Patent: October 4, 2022Assignee: Oxford Nanopore Technologies PLCInventor: Brian McKeown
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Patent number: 11352664Abstract: The invention relates to adaptors for sequencing nucleic acids. The adaptors may be used to generate single stranded constructs of nucleic acid for sequencing purposes. Such constructs may contain both strands from a double stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) template. The invention also relates to the constructs generated using the adaptors, methods of making the adaptors and constructs, as well as methods of sequencing double stranded nucleic acids.Type: GrantFiled: March 25, 2019Date of Patent: June 7, 2022Assignee: Oxford Nanopore Technologies PLCInventor: Brian McKeown
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Publication number: 20220064722Abstract: The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.Type: ApplicationFiled: June 23, 2021Publication date: March 3, 2022Applicant: Oxford Nanopore Technologies Ltd.Inventors: Lakmal Jayasinghe, John Hagan Pryce Bayley, Stephen Cheley, Brian McKeown, James White, James Clarke
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Publication number: 20210285035Abstract: Methods for obtaining a reaction volume having a predetermined copy number, such as a single copy, of a known nucleic acid molecule therein are disclosed. Such a reaction volume may be useful as a control for nucleic acid amplification reactions. Nucleic acid constructs useful in such methods are also described. The constructs include a first region and a second region, wherein the second region is flanked by first and second primer binding sites to allow amplification across the second region, and wherein a selectively cleavable region is located between the first and second regions, with the selectively cleavable region being flanked by third and fourth primer binding sites to allow amplification across the selectively cleavable region. The second region acts as a reporter to indicate the presence of the entire nucleic acid molecule; and the selectively cleavable region can be cleaved to separate the first region from the second region, leaving an isolated copy of the first region.Type: ApplicationFiled: November 23, 2016Publication date: September 16, 2021Inventor: Brian McKeown
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Patent number: 11078530Abstract: The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.Type: GrantFiled: September 12, 2018Date of Patent: August 3, 2021Assignee: Oxford Nanopore Technologies Ltd.Inventors: Lakmal Jayasinghe, John Hagan Pryce Bayley, Stephen Cheley, Brian McKeown, James White, James Anthony Clarke
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Publication number: 20200002761Abstract: The invention relates to adaptors for sequencing nucleic acids. The adaptors may be used to generate single stranded constructs of nucleic acid for sequencing purposes. Such constructs may contain both strands from a double stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) template. The invention also relates to the constructs generated using the adaptors, methods of making the adaptors and constructs, as well as methods of sequencing double stranded nucleic acids.Type: ApplicationFiled: September 12, 2019Publication date: January 2, 2020Applicant: Oxford Nanopore Technologies Ltd.Inventor: Brian McKeown
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Publication number: 20190376132Abstract: The invention relates to adaptors for sequencing nucleic acids. The adaptors may be used to generate single stranded constructs of nucleic acid for sequencing purposes. Such constructs may contain both strands from a double stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) template. The invention also relates to the constructs generated using the adaptors, methods of making the adaptors and constructs, as well as methods of sequencing double stranded nucleic acids.Type: ApplicationFiled: March 25, 2019Publication date: December 12, 2019Applicant: Oxford Nanopore Technologies Ltd.Inventor: Brian McKeown
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Publication number: 20190002972Abstract: The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.Type: ApplicationFiled: September 12, 2018Publication date: January 3, 2019Applicant: Oxford Nanopore Technologies Ltd.Inventors: Lakmal Jayasinghe, John Hagan Pryce Bayley, Stephen Cheley, Brian McKeown, James White, James Anthony Clarke
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Publication number: 20180291440Abstract: The invention relates to adaptors for sequencing nucleic acids. The adaptors may be used to generate single stranded constructs of nucleic acid for sequencing purposes. Such constructs may contain both strands from a double stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) template. The invention also relates to the constructs generated using the adaptors, methods of making the adaptors and constructs, as well as methods of sequencing double stranded nucleic acids.Type: ApplicationFiled: February 27, 2018Publication date: October 11, 2018Applicant: Oxford Nanopore Technologies Ltd.Inventor: Brian McKeown
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Patent number: 10077471Abstract: The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.Type: GrantFiled: September 18, 2015Date of Patent: September 18, 2018Assignee: Oxford Nanopore Technologies Ltd.Inventors: Lakmal Jayasinghe, John Hagan Pryce Bayley, Stephen Cheley, Brian McKeown, James White, James Anthony Clarke
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Patent number: 9885078Abstract: The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.Type: GrantFiled: August 8, 2014Date of Patent: February 6, 2018Assignee: Oxford Nanopore Technologies LimitedInventors: Lakmal Jayasinghe, John Hagan Pryce Bayley, Stephen Cheley, Brian McKeown, James White, James Anthony Clarke
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Publication number: 20170335384Abstract: The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.Type: ApplicationFiled: August 3, 2017Publication date: November 23, 2017Applicant: Oxford Nanopore Technologies Ltd.Inventors: Lakmal Jayasinghe, John Hagan Pryce Bayley, Stephen Cheley, Brian McKeown, James White, James Anthony Clarke
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Publication number: 20170321266Abstract: The invention relates to adaptors for sequencing nucleic acids. The adaptors may be used to generate single stranded constructs of nucleic acid for sequencing purposes. Such constructs may contain both strands from a double stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) template. The invention also relates to the constructs generated using the adaptors, methods of making the adaptors and constructs, as well as methods of sequencing double stranded nucleic acids.Type: ApplicationFiled: December 27, 2016Publication date: November 9, 2017Applicant: Oxford Nanopore Technologies Ltd.Inventor: Brian McKeown
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Publication number: 20160076092Abstract: The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.Type: ApplicationFiled: September 18, 2015Publication date: March 17, 2016Inventors: Lakmal JAYASINGHE, John Hagan Pryce BAYLEY, Stephen CHELEY, Brian MCKEOWN, James WHITE, James Anthony CLARKE
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Patent number: 9135315Abstract: Aspects include data masking in database operations including intercepting a database query and identifying masked data in the query. The masked data in the query is unmasked by substituting the masked data with equivalent real values. The unmasked query is sent to the database engine, and a result provided by the database engine in response to the unmasked query is intercepted. The values in the result that correspond to sensitive information are identified. The result is masked by substituting the values in the result corresponding to sensitive information with masked equivalents. The masked result is returned to a user.Type: GrantFiled: February 26, 2013Date of Patent: September 15, 2015Assignee: Internatonal Business Machines CorporationInventors: Pedro Barbas, Austin Clifford, Gareth Jenkins, Brian McKeown
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Publication number: 20150031020Abstract: The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.Type: ApplicationFiled: August 8, 2014Publication date: January 29, 2015Inventors: Lakmal JAYASINGHE, John Hagan Pryce BAYLEY, Stephen CHELEY, Brian MCKEOWN, James WHITE, James Anthony CLARKE
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Publication number: 20140051069Abstract: The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.Type: ApplicationFiled: August 16, 2013Publication date: February 20, 2014Applicant: OXFORD NANOPORE TECHNOLOGIES LIMITEDInventors: Lakmal JAYASINGHE, John Hagan Pryce BAYLEY, Stephen CHELEY, Brian MCKEOWN, James WHITE, James Anthony CLARKE
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Publication number: 20130282697Abstract: Aspects include data masking in database operations including intercepting a database query and identifying masked data in the query. The masked data in the query is unmasked by substituting the masked data with equivalent real values. The unmasked query is sent to the database engine, and a result provided by the database engine in response to the unmasked query is intercepted. The values in the result that correspond to sensitive information are identified. The result is masked by substituting the values in the result corresponding to sensitive information with masked equivalents. The masked result is returned to a user.Type: ApplicationFiled: February 26, 2013Publication date: October 24, 2013Applicant: INTERNATIONAL BUSINESS MACHINES CORPORATIONInventors: Pedro Barbas, Austin Clifford, Gareth Jenkins, Brian McKeown