Patents by Inventor Brian Miki
Brian Miki has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 7396978Abstract: The present invention is directed to a nucleic acid comprising a nucleotide sequence that encodes a protein that imparts a dull luster to a seed coat when expressed within seed-coat tissues and to transgenic seeds, plant cells and plants expressing the protein.Type: GrantFiled: June 4, 2004Date of Patent: July 8, 2008Assignee: Her Majesty the Queen in right of Canada, as represented by the Minister of Agriculture and Agri-FoodInventors: Brian Miki, Mark Gijzen, Shea Miller
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Patent number: 7303873Abstract: T-DNA tagging with a promoterless ?-glucuronidase (GUS) gene generated transgenic Nicotiana tabacum plants that expressed GUS activity either only in developing seed coats, or constitutively. Cloning and deletion analysis of the GUS fusion revealed that the promoter responsible for seed coat specificity was located in the plant DNA proximal to the GUS gene. Analysis of the region demonstrated that the seed coat-specificity of GUS expression in this transgenic plant resulted from T-DNA insertion next to a cryptic promoter. This promoter is useful in controlling the expression of genes to the developing seed coat in plant seeds. Similarly, cloning and characterization of the cryptic constitutive promoter revealed the occurrence of several cryptic regulatory regions. These regions include promoter, negative regulatory elements, transcriptional enhancers, core promoter regions, and translational enhancers and other regulatory elements.Type: GrantFiled: May 13, 2003Date of Patent: December 4, 2007Assignee: Her Majesty the Queen in Right of Canada as Represented by the Minister of Agriculture and Agri-FoodInventors: Brian Miki, Thérèse Ouellet, Jiro Hattori, Elizabeth Foster, Hélène Labbé, Teresa Martin-Heller, Lining Tian, Daniel Charles William Brown, Peijun Zhang, Keqiang Wu
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Patent number: 7151170Abstract: The present invention provides for a gene obtained during the induction of microspore embryogenisis. The protein encoded by this gene renders plant cells embryongenic, and increases the regenerative capacity of the plant cell. Also disclosed is the regulatory region of this gene and its use for directing the expression of a gene of interest within a suitable host cell.Type: GrantFiled: June 2, 2000Date of Patent: December 19, 2006Assignees: Plant Research International B.V., Her Majesty the Queen in Right of Canada, as represented by the Minister of Agriculture and Agri-Food CanadaInventors: Kim Boutilier, Therese Ouellet, Jan Custers, Jiro Hattori, Brian Miki, Michiel Van Lookeren Campagne
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Patent number: 6939958Abstract: This invention is directed to a regulatory region obtained from a wheat aleurone gene LtpW1. This regulatory region, truncated derivatives, mutations, or deletions of this regulatory region, can be used to express heterologous genes of interest within aleurone cells of a plant. Furthermore, this invention is directed to a truncated LtpW1 regulatory region that exhibits constitutive activity with both monocot and dicot plants. This invention is also directed to vectors comprising these regulatory regions operatively linked with a heterologous gene of interest, as well as plant cell cultures and transgenic plants comprising these vectors. A method for the preparation of a plant using the regulatory regions of this invention are also disclosed.Type: GrantFiled: October 13, 2000Date of Patent: September 6, 2005Assignee: Her Majesty the Queen in Right of Canada, as represented by the Minister of Agriculture and Agri-FoodInventors: John Simmonds, Leslie Cass, Linda Harris, Sharon Allard, Kamal Malik, Teresa Martin-Heller, Dan Brown, Ming Hu, Brian Miki, Keqiang Wu
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Publication number: 20050055742Abstract: An nucleotide sequence and that exhibits regulatory element activity is disclosed. The nucleotide sequence may be defined by SEQ ID NO:22, a nucleotide sequence that hybridizes to the nucleic acid sequence of SEQ ID NO:22, or a compliment thereof. Also disclosed is a chimeric construct comprising the nucleotide sequence operatively linked with a coding region of interest. A method of expressing a coding region of interest within a plant by introducing the chimeric construct described above, into the plant, and expressing the coding region of interest is also provided. Also disclosed are plants, seed, or plant cells comprising the chimeric construct as defined above.Type: ApplicationFiled: June 10, 2004Publication date: March 10, 2005Inventors: Brian Miki, Jiro Hattori, Teresa Martin-Heller, Helene Labbe, Kamal Malik, Elizabeth Foster, Keqiang Wu, Daniel Brown, Lining Tian, Therese Ouellet, Peijun Zhang, Elizabeth James, Pierre Fobert, Venkatram Iyer
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Publication number: 20050034195Abstract: The present invention is directed to an isolated genomic sequence that is differentially expressed within seed-coat tissues. This invention also relates to a promoter obtained from the genomic sequence that is differentially expressed in seed-coat tissues, and the use of this promoter for directing seed-coat specific expression of a gene of interest within transformed plant cells or plants. A method for modifying the luster of a seed coat is also provided.Type: ApplicationFiled: June 4, 2004Publication date: February 10, 2005Inventors: Brian Miki, Mark Gijzen, Shea Miller
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Publication number: 20040073022Abstract: T-DNA tagging with a promoterless &bgr;-glucuronidase (GUS) gene generated transgenic Nicotiana tabacum plants that expressed GUS activity either only in developing seed coats, or constitutively. Cloning and deletion analysis of the GUS fusion revealed that the promoter responsible for seed coat specificity was located in the plant DNA proximal to the GUS gene. Analysis of the region demonstrated that the seed coat-specificity of GUS expression in this transgenic plant resulted from T-DNA insertion next to a cryptic promoter. This promoter is useful in controlling the expression of genes to the developing seed coat in plant seeds. Similarly, cloning and characterization of the cryptic constitutive promoter revealed the occurrence of several cryptic regulatory regions. These regions include promoter, negative regulatory elements, transcriptional enhancers, core promoter regions, and translational enhancers and other regulatory elements.Type: ApplicationFiled: May 13, 2003Publication date: April 15, 2004Applicant: Her Majesty the Queen in Right of Canada as Rep.by the Minister of Agriculture and Agri-Food CanadaInventors: Brian Miki, Therese Ouellet, Jiro Hattori, Elizabeth Foster, Helene Labbe, Teresa Martin-Heller, Lining Tian, Daniel Charles William Brown, Peijun Zhang, Keqiang Wu
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Publication number: 20010047091Abstract: T-DNA tagging with a promoterless &bgr;-glucuronidase (GUS) gene generated transgenic Nicotiana tabacum plant that expressed GUS activity either only in developing seed coats, or constitutively. Cloning and deletion analysis of the GUS fusion revealed that the promoter responsible for seed coat specificity was located in the plant DNA proximal to the GUS gene. Analysis of the region demonstrated that the seed coat-specificity of GUS expression in this transgenic plant resulted from T-DNA insertion next to a cryptic promoter. This promoter is useful in controlling the expression of genes to the developing seed coat in plant seeds. Similarly, cloning and characterization of the cryptic constitutive promoter revealed the occurrence of several cryptic regulatory regions. These regions include promoter, negative regulatory elements, transcriptional enhancers, core promoter regions, and translational enhancers and other regulatory elements.Type: ApplicationFiled: December 22, 2000Publication date: November 29, 2001Inventors: Brian Miki, Therese Ouellet, Jiro Hattori, Elizabeth Foster, Helene Labbe, Teresa Martin-Heller, Lining Tian, Daniel Charles William Brown, Peijun Zhang, Keqiang Wu
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Patent number: 5824872Abstract: T-DNA tagging with a promoterless .beta.-glucuronidase (GUS) gene generated a transgenic Nicotiana tabacum plant that expressed GUS activity constitutively. The gene fusion has been cloned and sequenced. It has been re-inserted into N. tabacum by Agrobacterium-mediated transformation. The N. tabacum DNA upstream from the GUS gene was approximately 2 kb in length and showed no homology to known sequences. This DNA, which contains a constitutive promoter, is useful in controlling the expression of exogenous genes in transgenic plants of diverse plant species.Type: GrantFiled: February 1, 1996Date of Patent: October 20, 1998Inventors: Brian Miki, Jiro Hattori, Pierre Fobert, Venkatran N. Iyer
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Patent number: 5824863Abstract: T-DNA tagging with a promoterless .beta.-glucuronidase (GUS) gene generated a transgenic Nicotiana tabacum plant that expressed GUS activity only in developing seed coats. Cloning and deletion analysis of the GUS fusion revealed that the promoter responsible for seed coat specificity was located in the plant DNA proximal to the GUS gene. Analysis of the region demonstrated that the seed coat-specificity of GUS expression in this transgenic plant resulted from T-DNA insertion next to a cryptic promoter. This promotor is useful in controlling the expression of genes to the developing seed coat in plant seeds.Type: GrantFiled: May 15, 1995Date of Patent: October 20, 1998Assignees: Her Majesty the Queen in right of Canada, as represented by Agriculture, Agri-Food CanadaInventors: Brian Miki, V. N. Iyer, Pierre Fobert
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Patent number: 5756324Abstract: A novel DNA regulatory element that confers microspore-specific gene expression has been discovered, isolated, and characterized. The microspore-specific regulatory element can be used to control the expression of a foreign gene that disrupts the function of microspores. Thus, the control of pollen production can be achieved by using the microspore-specific regulatory element to produce male-sterile plants. Various methods can be used to restore male fertility in the F1 generation of such male-sterile plants. In addition, the microspore-specific regulatory element can be used to confer resistance to viral and insect pests.Type: GrantFiled: April 1, 1996Date of Patent: May 26, 1998Assignee: Pioneer Hi-Bred International, Inc.Inventors: Chris Baszczynski, Eric Barbour, Brian Miki, Jiro Hattori
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Patent number: 5659026Abstract: The Brassica ALS3 promoter is operably linked to a foreign structural gene to provide high level, and generally constitutive or tissue general expression of the structural gene in transformed plants. The Brassica ALS3 promoter exhibits a non-tissue-preferred mode of expression at a level comparable to, and in some cases higher than, the widely used CaMV 35S promoter. Accordingly, DNA constructs comprising the Brassica ALS3 promoter operably linked to any number of different gene coding regions can be used for constitutive and tissue-general expression of the gene in transformed plants. The Brassica ALS3 promoter is used to direct expression of agronomically important genes and selectable marker genes.Type: GrantFiled: March 24, 1995Date of Patent: August 19, 1997Assignee: Pioneer Hi-Bred InternationalInventors: Chris Baszczynski, Eric Barbour, Brian Miki
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Patent number: 5633438Abstract: A novel DNA regulatory element that confers microspore-specific gene expression has been discovered, isolated, and characterized. The microspore-specific regulatory element can be used to control the expression of a foreign gene that disrupts the function of microspores. Thus, the control of pollen production can be achieved by using the microspore-specific regulatory element to produce male-sterile plants. Various methods can be used to restore male fertility in the F1 generation of such male-sterile plants. In addition, the microspore-specific regulatory element can be used to confer resistance to viral and insect pests.Type: GrantFiled: November 22, 1994Date of Patent: May 27, 1997Assignee: Pioneer Hi-Bred InternationalInventors: Chris Baszczynski, Eric Barbour, Brian Miki, Jiro Hattori