Abstract: A method for making it possible to deliver nucleic acid sequences to a large fraction of, or even 99.9% and more of the cells in a human body without near certainty of killing the recipient. It can be applied to safely deliver any gene therapy. This invention comprises a set of known compounds, many of them already approved, combined in novel ways to prevent immune system reaction to levels of delivery vehicle (capsid or synthetic carrier) introduced into the body that can be 5 or more orders of magnitude higher than has been demonstrated to cause human death. When used in concert with the disclosed CRISPR expression control method, this method can improve expression and allow better control over the gene therapy's target activity.

Abstract: A composition and method comprising an anti-adjuvant such as DOI (an anti-inflammatory) together with any gene therapy plasmid is disclosed. A method for GHRH production in-vivo using a set of compositions and methods for use of those compositions is provided.

Abstract: A composition and method comprising an anti-adjuvant such as DOI (an anti-inflammatory) together with any gene therapy plasmid is disclosed. A method for GHRH production in-vivo using a set of compositions and methods for use of those compositions is provided.

Abstract: The present invention provides a method and device for the improvement of intraplexed assays. This improvement is based upon the use of multiple assay chemistries having different affinity constants (KA) for an analyte. The overall assay displays high precision and predictable behavior because ratios between SMPCS-IDGs having different affinity constants (KA) change based on concentration. The advantages of the Applicant's improved system relative to the system of the '290 patent are that the improved system (1) further increases the statistical significance of results from assays applied to single well samples, (2) improves compensation for multiple sources of error, (3) makes possible further increased precision for each analyte, and (4) improves correlation between instruments, even if the instruments have significantly varying responses to an identical stimulus, (4) makes possible improved determination of a margin of error.

Abstract: A method for treating HIV and other intra-cellular parasites and toxins using intrabodies delivered to leukocytes.

Abstract: A method for making suspended microarray readings from a single sample more reliably accurate. It can be applied to any assay system that uses discrete particles coupled with an assay. Most of these are fluidic systems that read the assay result using flow cytometry. However, other methods such as the distribution of tiny assay devices coupled with miniature transponders, where the sampling is of the environment, can also make use of this method. This invention combines a reference set of signal levels on particles with separately identified assays of one sample. By elimination of outliers, averaging, taking ratios of averages, and then taking ratios of assay signal levels against the reference set this method makes possible highly reliable diagnostics. When used standalone, the method uses different signal intensities to better calibrate an instrument. This method compensates for multiple sources of errors that can occur in this type of assay system.

Abstract: The present invention provides a method and device for the improvement of intraplexed assays. This improvement is based upon the use of multiple assay chemistries having different affinity constants (KA) for an analyte. The overall assay displays high precision and predictable behavior because ratios between SMPCS-IDGs having different affinity constants (KA) change based on concentration. The advantages of the Applicant's improved system relative to the system of the '290 patent are that the improved system (1) further increases the statistical significance of results from assays applied to single well samples, (2) improves compensation for multiple sources of error, (3) makes possible further increased precision for each analyte, and (4) improves correlation between instruments, even if the instruments have significantly varying responses to an identical stimulus, (4) makes possible improved determination of a margin of error.

Abstract: A method for making suspended microarray readings from a single sample statistically significant in a reliable manner. It can be applied to any assay system that uses discrete particles where the particles are coupled with an assay. Most of these systems suspend the particles in fluid and read the assay result using flow cytometry. However, other methods such as the distribution of tiny assay devices coupled with miniature transponders, where the sampling is of the environment, can also make use of this method. This invention combines multiple separately identified assays of one sample, where the multiple assays are for a single analyte and sets of assays have differing sensitivity to said analyte. By elimination of outliers, averaging, and taking ratios of averages, this method makes possible highly reliable diagnostics. This method compensates for multiple stochastic and non-stochastic sources of errors that can occur in this type of assay system.

Abstract: A method for making suspended microarray readings from a single sample statistically significant in a reliable manner. It can be applied to any assay system that uses discrete particles where the particles are coupled with an assay. Most of these systems suspend the particles in fluid and read the assay result using flow cytometry. However, other methods such as the distribution of tiny assay devices coupled with miniature transponders, where the sampling is of the environment, can also make use of this method. This invention combines multiple separately identified assays of one sample, where the multiple assays are for a single analyte and sets of assays have differing sensitivity to said analyte. By elimination of outliers, averaging, and taking ratios of averages, this method makes possible highly reliable diagnostics. This method compensates for multiple stochastic and non-stochastic sources of errors that can occur in this type of assay system.

Abstract: Agricultural operations by applying flexible manufacturing software, robotics and sensing techniques to agriculture. In manufacturing operations utilizing flexible machining and flexible assembly robots, work pieces flow through a fixed set of workstations on an assembly line. At different stations are located machine vision systems, laser based raster devices, radar, touch, photocell, and other methods of sensing; flexible robot armatures and the like are used to operate on them. This flexible agricultural automation turns that concept inside out, moving software programmable workstations through farm fields on mobile robots that can sense their environment and respond to it flexibly. The agricultural automation will make it possible for large scale farming to take up labor intensive farming practices which are currently only practical for small scale farming, improving land utilization efficiency, while lowering manpower costs dramatically.