Abstract: A patient ventilation system and a computing device-implemented method determine oxygen consumption in a mechanically ventilated subject includes receiving a signal representing a total flow at an inspiratory outlet of a ventilator, calculating a transport delay time between inspiratory and expiratory gas sample points in a patient ventilation circuitry, transmitting a signal to an inlet selector valve to selectively open a fluid path between the inspiratory gas sample point and an oxygen sensor or between the expiratory gas sample point and the oxygen sensor, receiving data representing oxygen content and carbon dioxide content over a period of time, calculating oxygen consumption data over the period of time from the data representing the total flow, oxygen content and carbon dioxide content and from the transport delay time, and displaying the oxygen consumption data over the period of time.

Abstract: The inventors have developed tools for quantifying the mitochondrial redox state of in vivo, in situ tissue using resonance Raman spectroscopy. The tissue is illuminated with an excitation beam that causes the tissue to scatter Raman-shifted light, which is collected and analyzed to produce coefficients representing the relative concentrations of different chromophores in the tissue. These relative concentrations indicate the redox state of whole mitochondria, hemoglobin oxygen saturation, myoglobin oxygen saturation, and/or redox state of individual cytochrome complexes in mitochondria of the in vivo, in situ tissue. Quantifiable information about these states and/or saturations can be used to assess tissue health, including organ (dys)function before, during, and after surgery. For example, this information can be used to predict impending cardiac failure, to guide surgical interventions, to monitor organ health after transplantation, or to guide post-operative care.

Abstract: A patient ventilation system and a computing device-implemented method determine oxygen consumption in a mechanically ventilated subject includes receiving a signal representing a total flow at an inspiratory outlet of a ventilator, calculating a transport delay time between inspiratory and expiratory gas sample points in a patient ventilation circuitry, transmitting a signal to an inlet selector valve to selectively open a fluid path between the inspiratory gas sample point and an oxygen sensor or between the expiratory gas sample point and the oxygen sensor, receiving data representing oxygen content and carbon dioxide content over a period of time, calculating oxygen consumption data over the period of time from the data representing the total flow, oxygen content and carbon dioxide content and from the transport delay time, and displaying the oxygen consumption data over the period of time.

Abstract: The inventors have developed tools for quantifying the mitochondrial redox state of in vivo, in situ tissue using resonance Raman spectroscopy. The tissue is illuminated with an excitation beam that causes the tissue to scatter Raman-shifted light, which is collected and analyzed to produce coefficients representing the relative concentrations of different chromophores in the tissue. These relative concentrations indicate the redox state of whole mitochondria, hemoglobin oxygen saturation, myoglobin oxygen saturation, and/or redox state of individual cytochrome complexes in mitochondria of the in vivo, in situ tissue. Quantifiable information about these states and/or saturations can be used to assess tissue health, including organ (dys)function before, during, and after surgery. For example, this information can be used to predict impending cardiac failure, to guide surgical interventions, to monitor organ health after transplantation, or to guide post-operative care.

Abstract: A cell culture support comprising a substrate, and a dual stimuli responsive block copolymer immobilized on the substrate, wherein the dual stimuli responsive block copolymer is both thermoresponsive and pH responsive. A method of culturing cells comprising the cell culture support having a dual stimuli responsive copolymer immobilized on a substrate, wherein the dual stimuli responsive copolymer is thermoresponsive and pH responsive; and growing the cells on the cell culture support. By lowering the temperature, cells are released from the cell culture support.

Abstract: Method and systems, for processing biological material, that contain a biological material in a vessel; add an aggregating agent to the material in the vessel and allow the material to separate into two or more distinct submaterials; extract one or more of the submaterials from the vessel; automatically transport one or more of the submaterials remaining in the vessel to a filtration device; and collect a resulting target retentate into a target retentate receptacle.

Abstract: The present invention relates to hydrogels and methods for producing and using the same. In particular, some embodiments of the invention relate to hydrogels and methods for patterning the same.

Abstract: The present invention relates to hydrogels and methods for producing and using the same. In particular, some embodiments of the invention relate to hydrogels and methods for patterning the same.

Abstract: A cell culture support comprising a substrate, and a dual stimuli responsive block copolymer immobilized on the substrate, wherein the dual stimuli responsive block copolymer is both thermoresponsive and pH responsive. A method of culturing cells comprising the cell culture support having a dual stimuli responsive copolymer immobilized on a substrate, wherein the dual stimuli responsive copolymer is thermoresponsive and pH responsive; and growing the cells on the cell culture support. By lowering the temperature, cells are released from the cell culture support.

Abstract: The present invention relates to hydrogels and methods for producing and using the same. In particular, some embodiments of the invention relate to hydrogels and methods for patterning the same.

Abstract: Method and systems, for processing biological material, that contain a biological material in a vessel; add an aggregating agent to the material in the vessel and allow the material to separate into two or more distinct submaterials; extract one or more of the submaterials from the vessel; automatically transport one or more of the submaterials remaining in the vessel to a filtration device; and collect a resulting target retentate into a target retentate receptacle.