Abstract: Techniques for automated determination or correction of count bias are based on nucleic acid base content on a finer grained scale than a bin of interest in a target sequence. The techniques include obtaining a target sequence with bins where relative abundances indicate a condition and raw counts Hj of reads, from a subject, which start at each locus j. A partition indicates a fine-grained window at a position relative to a current locus and multiple strata indicating different base contents. Each locus is attributed to one stratum k(j). An expected count of each stratum, E(k), is determined based on Hj for j belonging to the stratum and a number of loci in the target belonging to the stratum. A copy number of a bin is based on a sum of E(k(j)) in the bin. Output data indicates condition of the subject based at least partly on the copy number.

Abstract: According to some embodiments, so-called “NS-Tree” indexes may be used to calculate whether all (or a portion) of two independent tables are consistent. The NS-Tree indexes may be comprised of various elements, e.g., a synchronization time, a primary key, a hash of the complete record itself, and an aggregate value associated with each index entry (e.g., an accumulated XOR value). At any point in the index, an entry may possess the accumulated aggregate value of all key entries in the table up to that point. That aggregate value may be used to validate data consistency with another table(s) maintaining the same index. Due to the unique composition of the NS-Tree index, users can also validate two data sets within a ‘sub-range’ of the entire data set. According to other embodiments, NS-Tree indexes may also be applied to two different clusters of nodes by applying the NS-Trees at a ‘federated’ level.

Abstract: A method for determining pattern of inheritance in an embryo for a region of interest is provided, comprising receiving embryo, maternal and paternal sequence data; aligning the received sequence data to a reference genome; identifying a region of interest in the aligned embryo sequence data; identifying single nucleotide polymorphisms (SNPs) in the maternal sequence data, paternal sequence data, and the identified region of interest in the embryo sequence data; imputing missing gaps in the maternal sequence data and the paternal sequence data using an imputation reference; counting the number of SNPs that are common between the embryo and the mother/father in the identified region of interest for the embryo sequence data and a corresponding region on the maternal/paternal sequence data to determine a maternal/paternal contribution value; and classifying a pattern of inheritance for the embryo as maternal or paternal based on the relative contribution values between mother and father.

Abstract: A method for determining the genetic relationship of a conceptus with sperm and oocyte providers is provided, comprising receiving conceptus, sperm provider, and oocyte provider sequence data; aligning the received sequence data to a reference genome; identifying single nucleotide polymorphisms (SNPs) in the sperm provider, oocyte provider, and conceptus sequence data; imputing missing gaps in the sperm provider sequence data and the oocyte provider sequence data using an imputation reference; calculating a paternal consistency score between the sperm provider and the conceptus; calculating a maternal consistency score between the oocyte provider and conceptus; and classifying the sperm provider and/or the oocyte provider as related to the conceptus if the paternal consistency score and/or the maternal consistency score exceeds the predetermined threshold.

Abstract: A method for detecting ploidy in an embryo is provided, comprising receiving an embryo sequence data; aligning the received sequence data to a reference genome; identifying a region of interest in the aligned embryo sequence data; identifying single nucleotide polymorphisms (SMPs) in the sequence data by comparing the received sequence data to the aligned reference genome; determining a ploidy score comprising counting the number of observed SNPs in the region of interest; comparing the ploidy score to a predetermined threshold; and identifying the embryo as polyploid if the ploidy score is below the predetermined threshold.

Abstract: A method for verifying a genomic variant region in an embryo, is disclosed. Embryo sequencing data is received by one or more processors. The received embryo sequencing data is aligned to a reference genome, by the one or more processors. A genomic variant region is identified in the aligned embryo sequencing data, by the one or more processors. A number of single nucleotide variants (SNVs) is counted in the identified genomic variant region, by the one or more processors. The counted number of SNVs in the identified genomic variant region is normalized against a baseline count of SNVs for a reference region corresponding to the identified genomic variant region to generate a normalized SNV density for the genomic variant region, by the one or more processors. The identified genomic variant region is verified, by the one or more processors, if the normalized SNV density in the identified genomic variant region satisfies a tolerance criterion.

Abstract: According to some embodiments, so-called “NS-Tree” indexes may be used to calculate whether all (or a portion) of two independent tables are consistent. The NS-Tree indexes may be comprised of various elements, e.g., a synchronization time, a primary key, a hash of the complete record itself, and an aggregate value associated with each index entry (e.g., an accumulated XOR value). At any point in the index, an entry may possess the accumulated aggregate value of all key entries in the table up to that point. That aggregate value may be used to validate data consistency with another table(s) maintaining the same index. Due to the unique composition of the NS-Tree index, users can also validate two data sets within a ‘sub-range’ of the entire data set. According to other embodiments, NS-Tree indexes may also be applied to two different clusters of nodes by applying the NS-Trees at a ‘federated’ level.

Abstract: A digital video builder system with methodology for designer-controlled user interaction is described. In one embodiment, for example, a method comprises receiving, from interactive controls on a graphical user interface, configuration of a storyboard block of a storyboard of a target digital video to be created. The storyboard block represents a scene of the target digital video, comprises a media element slot and a scene parameter for configuring the media element slot, and corresponds to a scene specification having a plurality of scene variants for rendering the scene of the target digital video. The received configuration comprises a setting for the scene parameter. Based on the scene parameter setting, the method further includes automatically selecting one of the plurality of scene variants for use in rendering as part of the scene of the target digital video.

Abstract: Techniques for automated determination or correction of count bias are based on nucleic acid base content on a finer grained scale than a bin of interest in a target sequence. The techniques include obtaining a target sequence with bins where relative abundances indicate a condition and raw counts Hj of reads, from a subject, which start at each locus j. A partition indicates a fine-grained window at a position relative to a current locus and multiple strata indicating different base contents. Each locus is attributed to one stratum k(j). An expected count of each stratum, E(k), is determined based on Hj for j belonging to the stratum and a number of loci in the target belonging to the stratum. A copy number of a bin is based on a sum of E(k(j)) in the bin. Output data indicates condition of the subject based at least partly on the copy number.

Abstract: A digital video builder system with methodology for designer-controlled user interaction is described. In one embodiment, for example, a method comprises receiving, from interactive controls on a graphical user interface, configuration of a storyboard block of a storyboard of a target digital video to be created. The storyboard block represents a scene of the target digital video, comprises a media element slot and a scene parameter for configuring the media element slot, and corresponds to a scene specification having a plurality of scene variants for rendering the scene of the target digital video. The received configuration comprises a setting for the scene parameter. Based on the scene parameter setting, the method further includes automatically selecting one of the plurality of scene variants for use in rendering as part of the scene of the target digital video.

Abstract: The present invention relates to a vehicle that is converted from a stroller for carrying a passenger and cycle operated by a rider to a combined unit. More specifically, the invention relates to a stroller and a bicycle combined, physically and operationally, such that the operator of the cycle portion of the stroller-cycle can control operation of the stroller portion while the stroller-cycle is in motion.

Abstract: A cable splicing assembly for guiding a pair of control cables in a vehicle for pushing and riding. The vehicle has a pushing section and a riding section. The cable splicing assembly includes a splice housing having a circumferential opening at a first end and a pair of apertures at an opposite second end. The cable splicing assembly further includes a piston configured to slide axially within the splice housing between the opposite ends thereof in response to movement in the pair of control cables. The piston has an opening for guiding the pair of control cables through a longitudinal length. The cable splicing assembly further includes a housing cap mounted around the first end of the splice housing to close the circumferential opening of the splice housing and to form a cavity with the first end of the splice housing.

Abstract: The present invention relates to a vehicle that is converted from a stroller for carrying a passenger and cycle operated by a rider to a combined unit. More specifically, the invention relates to a stroller and a bicycle combined, physically and operationally, such that the operator of the cycle portion of the stroller-cycle can control operation of the stroller portion while the stroller-cycle is in motion.

Abstract: A braking and steering assembly in a vehicle having a pushing section and a riding section. The assembly includes an upper connection post containing braking and steering components for the riding section. The upper connection post has an upper end and a lower threaded end. The assembly further includes a lower connection post containing braking and steering components for the pushing section. The lower connection post has an aperture for threadedly receiving the lower threaded end of the upper connection post. The assembly also includes a rotatable front knob fixed to the upper end of the upper connection post. Rotatably tightening the front knob connects the lower end of the upper connection post with the upper end of the lower connection post. Braking and steering in the riding section controls braking and steering in the pushing section.

Abstract: A braking and steering assembly in a vehicle having a pushing section and a riding section. The assembly includes an upper connection post containing braking and steering components for the riding section. The upper connection post has an upper end and a lower threaded end. The assembly further includes a lower connection post containing braking and steering components for the pushing section. The lower connection post has an aperture for threadedly receiving the lower threaded end of the upper connection post. The assembly also includes a rotatable front knob fixed to the upper end of the upper connection post. Rotatably tightening the front knob connects the lower end of the upper connection post with the upper end of the lower connection post. Braking and steering in the riding section controls braking and steering in the pushing section.

Abstract: An openable caster having a mount cap, a mount housing pivotally connected to the mount cap, a post protruding from a top surface of the mount housing, a switch disposed on the mount cap and a slide release mechanism for locking and releasing the caster. The slide release mechanism has a flange disposed within the mount cap. The flange includes a recess with a bracket, the bracket being accessible from an underside of the mount housing. The flange is operationally connected to the switch. Actuation of the switch moves the slide release mechanism from a locked state, in which the caster is closed and a portion of the post is secured by the bracket, to a released state in which the post is released from the bracket.

Abstract: A vehicle for pushing and riding, the vehicle including a frame having a front portion and a rear portion. The front portion of the frame has a lower connection post containing braking and steering components and the rear portion of the frame has an upper connection post containing braking and steering components for the rear portion. The upper and lower connection posts are threadedly engaged within a steering portion of the frame. A hitch beam on the front portion forms part of the frame. A hitch clamp on the rear portion of the frame is fastened around the hitch beam for connecting the front portion and the rear portion of the frame. A pair of front wheels is operationally connected to the front portion of the frame. At least one rear wheel is operationally connected to the rear portion of the frame.

Abstract: The present invention relates to a vehicle that is converted from a stroller for carrying a passenger and cycle operated by a rider to a combined unit. More specifically, the invention relates to a stroller and a bicycle combined, physically and operationally, such that the operator of the cycle portion of the stroller-cycle can control operation of the stroller portion while the stroller-cycle is in motion.

Abstract: A vehicle for pushing and riding, the vehicle including a frame having a front portion and a rear portion. The front portion of the frame has a lower connection post containing braking and steering components and the rear portion of the frame has an upper connection post containing braking and steering components for the rear portion. The upper and lower connection posts are threadedly engaged within a steering portion of the frame. A hitch beam on the front portion forms part of the frame. A hitch clamp on the rear portion of the frame is fastened around the hitch beam for connecting the front portion and the rear portion of the frame. A pair of front wheels is operationally connected to the front portion of the frame. At least one rear wheel is operationally connected to the rear portion of the frame.