Abstract: Some embodiments of the methods and compositions provided herein relate to obtaining long read information from short reads of a target nucleic acid. Some embodiments include steps to selectively generate, mark, and amplify long nucleic acid fragments. Some embodiments include enriching for certain sequences in the long fragments with selection probes directed to certain genes throughout the genome and expressed regions with low mappability. Some embodiments also include fragmenting the long nucleic acid fragments into shorter fragments for sequencing, and informatically reconstructing a sequence of the target nucleic acid.

Abstract: Systems and methods for a bedside handle system having two-stage laser motion detection and wireless communication alert. The system detects and sends wireless alerts when a patient sits up and when the patient stands up. The system further includes a mobile app for receiving and displaying the alerts.

Abstract: A system for cleaning a filter mounted over an intake opening of an air handling unit. A conduit is secured to an elevated location of the unit and includes a plurality of spaced nozzles arrayed against the filter. A supply tube connects to the conduit for supplying a pressurized fluid through the nozzles in order to clear debris from an exterior of the mounted filter. The pressurized fluid includes either of a liquid (typically water) or a compressed air provided from any of a source or a supply reservoir. A pump is incorporated into the supply tube for pressurizing the fluid. A filter incorporated into the supply tube filters the pressurized fluid prior to delivery to spray issuing conduit. A processor/controller is provided in combination with an arrangement of control valves for providing timed sequenced washing of the filters. A collection basin is provided for recycling used liquid for re-delivery and re-use through the spray delivery conduits.

Abstract: Instrument and systems for applying ablative energy to epicardial tissue via a subxiphoid access surgical approach. The instrument has a head assembly sized and shaped for a subxiphoid surgical approach to a patient's heart and defines a contact face. The head assembly includes a paddle body, a first ablation electrode, and a second ablation electrode. The ablation electrodes are coupled to the paddle body in a spaced apart, spatially-fixed fashion. The ablation electrodes are exteriorly exposed at the contact face. A tubular member extends from the head assembly and maintains wiring connected to the ablation electrodes. The instrument is manipulable to locate the contact face on epicardial tissue of a patient's heart via a subxiphoid surgical approach, such as between the left and right pulmonary vein junctions of the posterior left atrium.

Abstract: A logic gate system for fault insertion testing can include a logic gate module having a plurality of input pins. The plurality of input pins can include an input signal pin configured to receive an input signal, a power supply input pin configured to receive power from a power supply, and a test input pin. The logic gate module can also include an output pin connected to the input pins via one or more logic gates. The logic gate system can include a power supply line connected to the power supply input pin and the test input pin. The logic gate system can also include a zero-ohm jumper resistor disposed between the power supply input pin and the test input pin. The zero-ohm resistor can be configured to be replaced with a low ohm resistor to allow reverse driving a voltage on the test input pin. The one or more logic gates can be configured to reverse an output at the output pin when the voltage on the test input pin is reverse driven.

Abstract: A system for cleaning a filter mounted over an intake opening of an air handling unit. A conduit is secured to an elevated location of the unit and includes a plurality of spaced nozzles arrayed against the filter. A supply tube connects to the conduit for supplying a pressurized fluid through the nozzles in order to clear debris from an exterior of the mounted filter. The pressurized fluid includes either of water or compressed air provided from any of a source or a supply reservoir. A pump is incorporated into the supply tube for pressurizing the fluid. A filter incorporated into the supply tube filters the pressurized fluid prior to delivery to spray issuing conduit.

Abstract: A logic gate system for fault insertion testing can include a logic gate module having a plurality of input pins. The plurality of input pins can include an input signal pin configured to receive an input signal, a power supply input pin configured to receive power from a power supply, and a test input pin. The logic gate module can also include an output pin connected to the input pins via one or more logic gates. The logic gate system can include a power supply line connected to the power supply input pin and the test input pin. The logic gate system can also include a zero-ohm jumper resistor disposed between the power supply input pin and the test input pin. The zero-ohm resistor can be configured to be replaced with a low ohm resistor to allow reverse driving a voltage on the test input pin. The one or more logic gates can be configured to reverse an output at the output pin when the voltage on the test input pin is reverse driven.

Abstract: System and computer-implemented method of permitting or arbitrating encrypted electronic transactions to occur among an intermediary and an author in an electronic data system only when an interaction occurs between the author and a candidate who was exposed by the intermediary to data published by the author. The author publishes criteria about a candidate, and the candidate posts information about the candidate, which is ingested and married to personally identifiable information about the candidate. A score is calculated indicating a correlation between the candidate and the published criteria, and an intermediary exposes the candidate to the criteria, which is tracked. When the candidate completes an interaction with the author, an encrypted transaction is permitted to occur between the author and the candidate through a proxy, without the author knowing the identity of the intermediary who facilitated the introduction.

Abstract: A physiological characteristic monitoring system includes a wearable attachment device, an electronic device, and a physiological characteristic sensor (e.g., a glucose sensor) including a portion that is insertable into subcutaneous tissue of a user to generate sensor signals indicating a physiological characteristic of the user. The electronic device includes a first connector configured to detachably couple to the physiological characteristic sensor to receive the sensor signals in a first configuration of the electronic device, a second connector configured to detachably couple to the wearable attachment device in a second configuration of the electronic device, a controller configured to determine the physiological characteristic of the user based on the sensor signals, and a display configured to display the physiological characteristic of the user.

Abstract: A physiological characteristic monitoring system includes a physiological characteristic sensor that observes a physiological characteristic and generates sensor signals based on the observation. The physiological characteristic sensor includes a sensor connector. The physiological characteristic monitoring system includes a wearable device to be worn by a user in a first configuration and having a connector to couple to the sensor connector in a second configuration. The wearable device includes a controller that receives the sensor signals from the physiological characteristic sensor in the second configuration and determines a current value of the physiological characteristic based on the sensor signals.

Abstract: System and computer-implemented method of permitting or arbitrating encrypted electronic transactions to occur among an intermediary and an author in an electronic data system only when an interaction occurs between the author and a candidate who was exposed by the intermediary to data published by the author. The author publishes criteria about a candidate, and the candidate posts information about the candidate, which is ingested and married to personally identifiable information about the candidate. A score is calculated indicating a correlation between the candidate and the published criteria, and an intermediary exposes the candidate to the criteria, which is tracked. When the candidate completes an interaction with the author, an encrypted transaction is permitted to occur between the author and the candidate through a proxy, without the author knowing the identity of the intermediary who facilitated the introduction.

Abstract: Systems and methods for a bedside handle system having two-stage laser motion detection and wireless communication alert. The system detects and sends wireless alerts when a patient sits up and when the patient stands up. The system further includes a mobile app for receiving and displaying the alerts.

Abstract: Systems and methods for a bedside handle system having two-stage laser motion detection and wireless communication alert. The system detects and sends wireless alerts when a patient sits up and when the patient stands up. The system further includes a mobile app for receiving and displaying the alerts.

Abstract: A system and method for obtaining user approval for transactions. The system includes an authorization system that is communicably coupled to one or more transaction computer systems that execute at least some transactions that require user approval. When a transaction system is seeking to execute a transaction that requires user approval, the transaction system sends a user approval request to the authorization system. The authorization system then retrieves the user's communication preference from a database and transmits an affirmatively actionable message to the user according to the retrieved communication preference. The messages can include an email with an approval link to click or a text message that the can be replied to. When the authorization system receives confirmation from the user that the user approves the request, the authorization system then transmits approval for the transaction to the appropriate transaction system, which then proceeds to execute the transaction.

Abstract: A method of measuring a physiological characteristic of a user obtains first calibrating sensor data indicative of an analyte level of the user, and produced in response to operation of a continuous analyte sensor device during a calibration period, and obtains second calibrating sensor data indicative of status or condition of the user, and produced in response to operation of a noninvasive sensor device, concurrently with operation of the continuous analyte sensor device, during the calibration period. A correlation measurement is calculated by comparing the first calibrating sensor data against the second calibrating sensor data. When the correlation measurement exceeds a threshold value, a transfer function is generated to calculate estimated values of the analyte level as a function of sensor data from the noninvasive sensor device. The transfer function is provided to a client device associated with the user.

Abstract: Instrument and systems for applying ablative energy to epicardial tissue via a subxiphoid access surgical approach. The instrument has a head assembly sized and shaped for a subxiphoid surgical approach to a patient's heart and defines a contact face. The head assembly includes a paddle body, a first ablation electrode, and a second ablation electrode. The ablation electrodes are coupled to the paddle body in a spaced apart, spatially-fixed fashion. The ablation electrodes are exteriorly exposed at the contact face. A tubular member extends from the head assembly and maintains wiring connected to the ablation electrodes. The instrument is manipulable to locate the contact face on epicardial tissue of a patient's heart via a subxiphoid surgical approach, such as between the left and right pulmonary vein junctions of the posterior left atrium.

Abstract: Instrument and systems for applying ablative energy to epicardial tissue via a subxiphoid access surgical approach. The instrument has a head assembly sized and shaped for a subxiphoid surgical approach to a patient's heart and defines a contact face. The head assembly includes a paddle body, a first ablation electrode, and a second ablation electrode. The ablation electrodes are coupled to the paddle body in a spaced apart, spatially-fixed fashion. The ablation electrodes are exteriorly exposed at the contact face. A tubular member extends from the head assembly and maintains wiring connected to the ablation electrodes. The instrument is manipulable to locate the contact face on epicardial tissue of a patient's heart via a subxiphoid surgical approach, such as between the left and right pulmonary vein junctions of the posterior left atrium.

Abstract: This invention is method to protect secondary media from the environmental conditions that exist while performing line demarcation. Particularly, this invention relates to the protection of dry media as it is applied to previously applied, wet media. More particularly, this invention relates to the protection of reflective glass beads as they are applied to freshly applied wet paint.

Abstract: System and computer-implemented method of permitting or arbitrating encrypted electronic transactions to occur among an intermediary and an author in an electronic data system only when an interaction occurs between the author and a candidate who was exposed by the intermediary to data published by the author. The author publishes criteria about a candidate, and the candidate posts information about the candidate, which is ingested and married to personally identifiable information about the candidate. A score is calculated indicating a correlation between the candidate and the published criteria, and an intermediary exposes the candidate to the criteria, which is tracked. When the candidate completes an interaction with the author, an encrypted transaction is permitted to occur between the author and the candidate through a proxy, without the author knowing the identity of the intermediary who facilitated the introduction.

Abstract: A method, computer system, and computer program product for a standalone demonstration of an application. Requests sent from the application running on a browser to a server data processing system are detected. Responses received from the server data processing system for the requests sent from the application are detected. The requests and the responses for the application are stored in a data structure. The requests and the responses enable simulating the server data processing system to perform the standalone demonstration of the application running on the browser on a client data processing system using the data structure without communicating with the server data processing system.