Abstract: The disclosure relates to modified orthopoxvirus vectors, as well as methods of using the same for the treatment of various cancers. The disclosure provides modified orthopoxvirus vectors that exhibit various beneficial therapeutic activities, including enhanced oncolytic activity, spread of infection, immune evasion, tumor persistence, capacity for incorporation of exogenous DNA sequences and safety. The viruses we have discovered are also amenable to large scale manufacturing protocols.

Abstract: A fire suppression system for producing oxygen-depleted air includes a fuel cell stack formed from a plurality of fuel cells for providing power to an associated load, and a controller coupled to the plurality of fuel cells, wherein the controller is configured to regulate current output from the plurality of fuel cells to maintain a prescribed percentage level of oxygen depleted air in an exhaust stream of the plurality of fuel cells. Further, a method for maintaining an updated polarization curve for a fuel cell includes commanding step-and-hold air flow commands and associated electrical current limit commands to the fuel cell system. Upon the fuel cell reaching each successive step-and-hold steady state condition, electrical current and voltage pairs are stored and plotted to form the real-time polarization curve.