Abstract: A method for providing a preferentially segmented digital image of a specimen image having predetermined characteristics and containing an object of interest. A specimen image is obtained through a microscope set at a magnification value and digitized to create a digital specimen image. Preferential enhancement values are determined based upon the magnification value and the specimen characteristics, and then the digital specimen image is enhanced using the preferential enhancement values to accentuate the object dark features. This creates a dark feature enhanced image which is then binarized to yield an edge mask that consists of black object silhouettes on a white background. Detection of the edges of the object contained in the edge mask is then performed to create an edge overlay that can be used to obtain morphometric measurements or combined with the digital specimen image to create a preferentially segmented digital image suitable for densitometric measurement.