Abstract: The present invention provides the promoter clone discovery of a glucoamylase gene of a starch utilizing yeast strain Schwanniomyces castellii. The isolated glucoamylase promoter is an inducible promoter, which can regulate strong gene expression in starch culture medium.

Abstract: The promoter of the ribosomal protein gene L25 is operably linked to a structural gene. This chimeric gene is placed in an expression vector. The expression vector containing the chimeric gene is used to transform plants cells and plants. Seeds are obtained from the transformed plants. A product, such as a protein, encoded by the structural gene is isolated from the transformed plant cells and plants.

Abstract: The present invention provides the promoter clone discovery of an alpha-amylase gene of a starch utilizing yeast strain Schwanniomyces castellii. The isolated alpha-amylase promoter is an inducible promoter, which can regulate strong gene expression in starch culture medium.

Abstract: This invention provides transgenic plants that have increased or decreased activity of uridine 5′-diphospho-galactose 4-epimerase. Controlling the level of uridine 5′-diphospho-galactose 4-epimerase in the transgenic plants is used to regulate the nutritional profile of the plant by controlling the use of glucose and/or galactose. An isolated polynucleotide coding for the potato uridine 5′-diphospho-galactose 4-epimerase protein, its antisense equivalent and the nucleic acid sequence of potato uridine 5′-diphospho-galactose 4-epimerase are also provided. The present invention also provides a method for reducing the activity of uridine 5′-diphospho-galactose 4-epimerase activity in plants. The present invention also provides a method of producing bulk quantities of uridine 5′-diphospho-galactose 4-epimerase enzyme in transgenic plants.

Abstract: The present invention provides the promoter clone discovery of an alpha-amylase gene of a starch utilizing yeast strain Schwanniomyces castellii. The isolated alpha-amylase promoter is an inducible promoter, which can regulate strong gene expression in starch culture medium.

Abstract: The invention includes polynucleotide constructs that contain a polynucleotide promoter sequence from the phosphoglycerate kinase 1 (pgk1) gene from Rhizopus oryzae. The invention also includes methods of expressing a polypeptide in a host cell using a polynucleotide construct containing a 3-phosphoglycerate kinase (pgk) gene promoter sequence operably linked to a polynucleotide encoding a polypeptide of interest. The invention also includes a system for producing a polypeptide. The system has a first polynucleotide sequence containing a 3-phosphoglycerate kinase gene promoter sequence isolated from a fungal species, and a second polynucleotide sequence which has been isolated from a second species and which encodes a polypeptide. A host cell contains the first polynucleotide sequence operably linked to the second polynucleotide sequence. The host cell is from a different species than the fungal species from which the first polynucleotide sequence is isolated.

Abstract: There are disclosed novel pin1 gene promoter isoforms and amt gene promoter isoforms. The promoters and their functional elements may be used independently or in combination with one or more enhancer elements to increase or otherwise manipulate gene expression. The promoters disclosed may be used in Controlled Environment Agriculture for heterologous protein production. Also disclosed are methods for using the promoter and promoter elements of the instant invention, as well as vectors and transgenic plants comprising the same.

Abstract: The present invention provides the promoter clone discovery of a glucoamylase gene of a starch utilizing yeast strain Schwanniomyces castellii. The isolated glucoamylase promoter is an inducible promoter, which can regulate strong gene expression in starch culture medium.

Abstract: The present invention provides the promoter clone discovery of an alpha-amylase gene of a starch utilizing yeast strain Schwanniomyces castellii. The isolated alpha-amylase promoter is an inducible promoter, which can regulate strong gene expression in starch culture medium.

Abstract: An integrated system for commercial production of a heterologous protein in transgenic plants under conditions of controlled environment agriculture (CEA) is provided. CEA comprises growth of plants under defined environmental conditions, preferably in a greenhouse, to optimize growth of the transgenic plant as well as expression of the gene encoding the heterologous protein. The transgenic plants used in the present invention are transformed with an expression vector comprising a CEA promoter operably linked to a gene encoding the heterologous protein of interest, wherein the CEA promoter is selected to maximize heterologous protein production under the defined environmental conditions of CEA.

Abstract: Synergistically functional, yet separable, cis-acting enhancer elements from the rpL34 promoter are disclosed. These enhancer elements of the instant invention may be used in combination with a plurality of promoters to increase gene expression without affecting the intrinsic specificity of the promoters. Also disclosed are methods for using the enhancer elements of the instant invention as well as vectors and transgenic plants comprising the enhancer elements.