Patents by Inventor Brian T. Chait
Brian T. Chait has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Publication number: 20240002475Abstract: Provided are compositions and methods for producing large repertoires of recombinant single domain antibodies with high affinities and specificities against any antigen. Included are methods for making and identifying single domain antibodies produced by camelids, the single domain antibodies themselves, modifications of the nanobodies, expression vectors encoding the nanobodies, cDNAs encoding the nanobodies, cells comprising the expression vectors and/or cDNA, and methods of making the single domain antibodies re-combinantly Antigen-specific single domain antibodies and antigen binding fragments thereof having a Kd for the antigen in a sub-micromolar range are provided. The use of Protein M in isolating Ag-specific HCAbs, and digesting the isolated HCAbs using IdeS protease, is an aspect of this disclosure.Type: ApplicationFiled: December 3, 2021Publication date: January 4, 2024Inventors: Brian T. CHAIT, Michael P. ROUT
-
Publication number: 20230331824Abstract: Single-domain antibodies that bind the severe acute respiratory syndrome corona virus 2 (SARS-CoV-2) spike protein are disclosed. The single-domain antibodies include binding domains that bind epitopes of the Spike ectodomain inside and outside the receptor binding domain. The single-domain antibodies can be used for multiple purposes including in the research, diagnosis, and prophylactic or therapeutic treatment of COVID-19.Type: ApplicationFiled: August 20, 2021Publication date: October 19, 2023Applicants: The Rockefeller University, Seattle Children's Hospital d/b/a Seattle Children's Research Institute, New York UniversityInventors: Brian T. Chait, Michael P. Rout, John Aitchison, Fred David Mast, Jean Paul Olivier, David Fenyo
-
Patent number: 11447891Abstract: Provided are compositions and methods for producing large repertoires of recombinant nanobodies with high affinities and specificities against any antigen. Included are methods for making and identifying nanobodies produced by camelids, the nanobodies themselves, modifications of the nanobodies, expression vectors encoding the nanobodies, cDNAs encoding the nanobodies, cells comprising the expression vectors and/or cDNA, and methods of making the nanobodies recombinantly. Antigen-specific nanobodies and antigen binding fragments thereof having a Kd for the antigen in a sub-micromolar range are provided.Type: GrantFiled: August 13, 2019Date of Patent: September 20, 2022Assignee: The Rockefeller UniversityInventors: Michael P. Rout, Brian T. Chait
-
Publication number: 20190391159Abstract: Provided are compositions and methods for producing large repertoires of recombinant nanobodies with high affinities and specificities against any antigen. Included are methods for making and identifying nanobodies produced by camelids, the nanobodies themselves, modifications of the nanobodies, expression vectors encoding the nanobodies, cDNAs encoding the nanobodies, cells comprising the expression vectors and/or cDNA, and methods of making the nanobodies recombinantly. Antigen-specific nanobodies and antigen binding fragments thereof having a Kd for the antigen in a sub-micromolar range are provided.Type: ApplicationFiled: August 13, 2019Publication date: December 26, 2019Inventors: Michael P. Rout, Brian T. Chait
-
Publication number: 20170212130Abstract: Provided are compositions and methods for producing large repertoires of recombinant nanobodies with high affinities and specificities against any antigen. Included are methods for making and identifying nanobodies produced by camelids, the nanobodies themselves, modifications of the nanobodies, expression vectors encoding the nanobodies, cDNAs encoding the nanobodies, cells comprising the expression vectors and/or cDNA, and methods of making the nanobodies recombinantly. Antigen-specific nanobodies and antigen binding fragments thereof having a Kd for the antigen in a sub-micromolar range are provided.Type: ApplicationFiled: June 25, 2015Publication date: July 27, 2017Inventors: Michael P. Rout, Brian T. Chait
-
Patent number: 9299550Abstract: An ion trap includes a containment region for containing ions, and a plurality of electrodes positioned on a regular polyhedral structure encompassing the containment region. An electrode is positioned on each vertex of the encompassing structure and at least one of the polygonal surfaces includes additional electrodes configured to form a plurality of quadrupoles on the surface. Alternating RF voltage is applied to the plurality of electrodes, so that directly neighboring electrodes are of equal amplitude and opposite polarity at any point in time. This configuration on the polyhedral structure forms a potential barrier for repelling the ions from each of the regular polygonal surfaces and containing them in the trap. Mass selective filters can be formed from the quadrupoles for parallel mass analysis in different m/z windows. Application of a small DC potential to a plate electrode outside the quadrupoles preferentially depletes single charged ions for enhanced signal-to-noise analysis.Type: GrantFiled: September 8, 2015Date of Patent: March 29, 2016Assignee: THE ROCKEFELLER UNIVERSITYInventors: Andrew N. Krutchinsky, Vadim Sherman, Herbert Cohen, Brian T. Chait
-
Patent number: 9240310Abstract: An ion transfer device for transferring ions emerging from an electrospray ion source at atmosphere to a vacuum chamber includes an inner surface in the shape of a diverging conical duct. The ion transfer device has an entrance aperture for positioning proximate the exit port of the electrospray ion source emitter, the entrance aperture receiving the electrosprayed ions from the exit port of the electrospray ion source emitter at atmosphere, the diverging conical duct being an electrode toward which the ions migrate and having an exit aperture with an inner diameter larger than an inner diameter of its entrance aperture, the exit aperture enclosed in the vacuum chamber, the diverging conical duct transporting the ions from atmosphere to vacuum. The vacuum chamber can be a chamber of a vacuum housing enclosing a mass analyzer.Type: GrantFiled: April 28, 2015Date of Patent: January 19, 2016Assignee: THE ROCKEFELLER UNIVERSITYInventors: Andrew N. Krutchinsky, Julio Padovan, Herbert Cohen, Brian T. Chait
-
Publication number: 20160005579Abstract: An ion trap includes a containment region for containing ions, and a plurality of electrodes positioned on a regular polyhedral structure encompassing the containment region. An electrode is positioned on each vertex of the encompassing structure and at least one of the polygonal surfaces includes additional electrodes configured to form a plurality of quadrupoles on the surface. Alternating RF voltage is applied to the plurality of electrodes, so that directly neighboring electrodes are of equal amplitude and opposite polarity at any point in time. This configuration on the polyhedral structure forms a potential barrier for repelling the ions from each of the regular polygonal surfaces and containing them in the trap. Mass selective filters can be formed from the quadrupoles for parallel mass analysis in different m/z windows. Application of a small DC potential to a plate electrode outside the quadrupoles preferentially depletes single charged ions for enhanced signal-to-noise analysis.Type: ApplicationFiled: September 8, 2015Publication date: January 7, 2016Inventors: Andrew N. Krutchinsky, Vadim Sherman, Herbert Cohen, Brian T. Chait
-
Publication number: 20150318155Abstract: An ion transfer device for transferring ions emerging from an electrospray ion source at atmosphere to a vacuum chamber includes an inner surface in the shape of a diverging conical duct. The ion transfer device has an entrance aperture for positioning proximate the exit port of the electrospray ion source emitter, the entrance aperture receiving the electrosprayed ions from the exit port of the electrospray ion source emitter at atmosphere, the diverging conical duct being an electrode toward which the ions migrate and having an exit aperture with an inner diameter larger than an inner diameter of its entrance aperture, the exit aperture enclosed in the vacuum chamber, the diverging conical duct transporting the ions from atmosphere to vacuum. The vacuum chamber can be a chamber of a vacuum housing enclosing a mass analyzer.Type: ApplicationFiled: April 28, 2015Publication date: November 5, 2015Inventors: Andrew N. Krutchinsky, Julio Padovan, Herbert Cohen, Brian T. Chait
-
Patent number: 9129789Abstract: An ion trap includes a containment region for containing ions, and a plurality of electrodes positioned on a regular polyhedral structure encompassing the containment region. An electrode is positioned on each vertex of the encompassing structure and at least one of the polygonal surfaces includes additional electrodes configured to form a plurality of quadrupoles on the surface. Alternating RF voltage is applied to the plurality of electrodes, so that directly neighboring electrodes are of equal amplitude and opposite polarity at any point in time. This configuration on the polyhedral structure forms a potential barrier for repelling the ions from each of the regular polygonal surfaces and containing them in the trap. Mass selective filters can be formed from the quadrupoles for parallel mass analysis in different m/z windows. Application of a small DC potential to a plate electrode outside the quadrupoles preferentially depletes single charged ions for enhanced signal-to-noise analysis.Type: GrantFiled: September 23, 2014Date of Patent: September 8, 2015Assignee: THE ROCKEFELLER UNIVERSITYInventors: Andrew N. Krutchinsky, Vadim Sherman, Herbert Cohen, Brian T. Chait
-
Patent number: 9048079Abstract: An ion transfer device for transferring ions emerging from an electrospray ion source at atmosphere to a vacuum chamber includes an inner surface in the shape of a diverging conical duct. The ion transfer device has an entrance aperture for positioning proximate the exit port of the electrospray ion source emitter, the entrance aperture receiving the electrosprayed ions from the exit port of the electrospray ion source emitter at atmosphere, the diverging conical duct being an electrode toward which the ions migrate and having an exit aperture with an inner diameter larger than an inner diameter of its entrance aperture, the exit aperture enclosed in the vacuum chamber, the diverging conical duct transporting the ions from atmosphere to vacuum. The vacuum chamber can be a chamber of a vacuum housing enclosing a mass analyzer.Type: GrantFiled: March 14, 2013Date of Patent: June 2, 2015Assignee: THE ROCKEFELLER UNIVERSITYInventors: Andrew N. Krutchinsky, Julio Padovan, Herbert Cohen, Brian T. Chait
-
Publication number: 20150041640Abstract: An ion trap includes a containment region for containing ions, and a plurality of electrodes positioned on a regular polyhedral structure encompassing the containment region. An electrode is positioned on each vertex of the encompassing structure and at least one of the polygonal surfaces includes additional electrodes configured to form a plurality of quadrupoles on the surface. Alternating RF voltage is applied to the plurality of electrodes, so that directly neighboring electrodes are of equal amplitude and opposite polarity at any point in time. This configuration on the polyhedral structure forms a potential barrier for repelling the ions from each of the regular polygonal surfaces and containing them in the trap. Mass selective filters can be formed from the quadrupoles for parallel mass analysis in different m/z windows. Application of a small DC potential to a plate electrode outside the quadrupoles preferentially depletes single charged ions for enhanced signal-to-noise analysis.Type: ApplicationFiled: September 23, 2014Publication date: February 12, 2015Inventors: Andrew N. Krutchinsky, Vadim Sherman, Herbert Cohen, Brian T. Chait
-
Patent number: 8866076Abstract: An ion trap includes a containment region for containing ions, and a plurality of electrodes positioned on a regular polyhedral structure encompassing the containment region. An electrode is positioned on each vertex of the encompassing structure and at least one of the polygonal surfaces includes additional electrodes configured to form a plurality of quadrupoles on the surface. Alternating RF voltage is applied to the plurality of electrodes, so that directly neighboring electrodes are of equal amplitude and opposite polarity at any point in time. This configuration on the polyhedral structure forms a potential barrier for repelling the ions from each of the regular polygonal surfaces and containing them in the trap. Mass selective filters can be formed from the quadrupoles for parallel mass analysis in different m/z windows. Application of a small DC potential to a plate electrode outside the quadrupoles preferentially depletes single charged ions for enhanced signal-to-noise analysis.Type: GrantFiled: December 20, 2013Date of Patent: October 21, 2014Assignee: The Rockefeller UniversityInventors: Andrew N. Krutchinsky, Vadim Sherman, Herbert Cohen, Brian T. Chait
-
Publication number: 20140246582Abstract: An ion trap includes a containment region for containing ions, and a plurality of electrodes positioned on a regular polyhedral structure encompassing the containment region. An electrode is positioned on each vertex of the encompassing structure and at least one of the polygonal surfaces includes additional electrodes configured to form a plurality of quadrupoles on the surface. Alternating RF voltage is applied to the plurality of electrodes, so that directly neighboring electrodes are of equal amplitude and opposite polarity at any point in time. This configuration on the polyhedral structure forms a potential barrier for repelling the ions from each of the regular polygonal surfaces and containing them in the trap. Mass selective filters can be formed from the quadrupoles for parallel mass analysis in different m/z windows. Application of a small DC potential to a plate electrode outside the quadrupoles preferentially depletes single charged ions for enhanced signal-to-noise analysis.Type: ApplicationFiled: December 20, 2013Publication date: September 4, 2014Applicant: The Rockefeller UniversityInventors: Andrew N. Krutchinsky, Vadim Sherman, Herbert Cohen, Brian T. Chait
-
Publication number: 20140217281Abstract: An ion transfer device for transferring ions emerging from an electrospray ion source at atmosphere to a vacuum chamber includes an inner surface in the shape of a diverging conical duct. The ion transfer device has an entrance aperture for positioning proximate the exit port of the electrospray ion source emitter, the entrance aperture receiving the electrosprayed ions from the exit port of the electrospray ion source emitter at atmosphere, the diverging conical duct being an electrode toward which the ions migrate and having an exit aperture with an inner diameter larger than an inner diameter of its entrance aperture, the exit aperture enclosed in the vacuum chamber, the diverging conical duct transporting the ions from atmosphere to vacuum. The vacuum chamber can be a chamber of a vacuum housing enclosing a mass analyzer.Type: ApplicationFiled: March 14, 2013Publication date: August 7, 2014Applicant: THE ROCKEFELLER UNIVERSITYInventors: Andrew N. Krutchinsky, Julio Padovan, Herbert Cohen, Brian T. Chait
-
Patent number: 8637817Abstract: An ion trap includes a containment region for containing ions, and a plurality of electrodes positioned on a regular polyhedral structure encompassing the containment region. An electrode is positioned on each vertex of the encompassing structure and at least one of the polygonal surfaces includes additional electrodes configured to form a plurality of quadrupoles on the surface. Alternating RF voltage is applied to the plurality of electrodes, so that directly neighboring electrodes are of equal amplitude and opposite polarity at any point in time. This configuration on the polyhedral structure forms a potential barrier for repelling the ions from each of the regular polygonal surfaces and containing them in the trap. Mass selective filters can be formed from the quadrupoles for parallel mass analysis in different m/z windows. Application of a small DC potential to a plate electrode outside the quadrupoles preferentially depletes single charged ions for enhanced signal-to-noise analysis.Type: GrantFiled: March 1, 2013Date of Patent: January 28, 2014Inventors: Andrew N. Krutchinsky, Vadim Sherman, Herbert Cohen, Brian T. Chait
-
Patent number: 8227198Abstract: The present invention is method of determining whether or not associations between a given protein and other proteins in a cell are specific.Type: GrantFiled: May 12, 2011Date of Patent: July 24, 2012Assignee: The Rockefeller UniversityInventors: Brian T. Chait, Alan J. Tackett, Jeffrey A. DeGrasse, Marlene Oeffinger, Michael P. Rout, Matthew D. Sekedat
-
Publication number: 20110217718Abstract: The present invention is method of determining whether or not associations between a given protein and other proteins in a cell are specific.Type: ApplicationFiled: May 12, 2011Publication date: September 8, 2011Applicant: THE ROCKEFELLER UNIVERSITYInventors: Brian T. Chait, Alan J. Tackett, Jeffrey A. DeGrasse, Marlene Oeffinger, Michael P. Rout, Matthew D. Sekedat
-
Patent number: 7968299Abstract: The present invention is method of determining whether or not associations between a given protein and other proteins in a cell are specific.Type: GrantFiled: August 21, 2006Date of Patent: June 28, 2011Assignee: The Rockefeller UniversityInventors: Brian T. Chait, Alan J. Tackett, Jeffrey A. DeGrasse, Marlene Oeffinger, Michael P. Rout, Matthew D. Sekedat
-
Patent number: 7341874Abstract: This invention provides for novel methods of identifying covalent modifications of an amino acid residue in a polypeptide chain by detecting mass differences.Type: GrantFiled: March 2, 2004Date of Patent: March 11, 2008Assignees: The Rockefeller University, The Scripps Research InstituteInventors: Brian T. Chait, Ronald Beavis, Rong Wang, Stephen B. H. Kent