Abstract: Purifying target biomolecules, such as nucleic acids or proteins, from a biological source is a time intensive process and is typically performed by a skilled technician or scientist owing to the highly technical nature of the work. Systems, devices, and methods disclosed herein enable the automated bioprocessing and purification of target biomolecules from a biological source. For example, an instrument and disposable cartridge are provided for automatedly isolating and purifying nucleic acids (such as plasmid DNA from a bacterial culture) or for isolating protein from any biological sample. Such an exemplary instrument and cartridge can work in concert to timely release, mix, and move the target biomolecule and various reagents and buffers through a target biomolecule purification process, resulting in a purified target biomolecule with less manual oversight than traditional approaches.

Abstract: The present invention provides an improved vehicle seat pan and a method for controlling vertical deflection in a vehicle seat pan. A vehicle seat pan in accordance with the present invention includes a frame adapted to be mounted to a vehicle body. A suspension web for supporting a seat cushion is mounted to the frame. A vertical deflection controller is coupled between the web and the frame. The controller may be a leaf spring. Additionally, the controller may be a coil spring and a metal strap that limits the extension of the spring. A method for controlling vertical deflection in a vehicle seat pan is also provided. The method includes the step of mounting a substantially horizontal suspension web to a seat frame. The method further includes the step of coupling a vertical deflection controller between the web and the frame.