Abstract: A temperature regulated hybridization chamber which includes an outer container which defines a first chamber for receiving a first fluid having a predetermined temperature. The hybridization chamber includes an inner container having a generally horizontal bottom wall with a periphery and an inner container side wall extending generally upwardly from the periphery of the inner container bottom wall. The inner container bottom wall and the inner container side wall define a second chamber for receiving a second fluid. The inner container is positioned within the first chamber with the inner container bottom wall and the inner container side wall being spaced from the outer container bottom wall and the outer container side wall, respectively, such that when the first fluid is positioned within the first chamber the first fluid is in engagement with the inner container bottom wall and inner container side wall.