Abstract: The invention relates to G protein-coupled receptor (GPCR) microarrays on porous substrates for structural or functional analyses of GPCRs, and methods of preparing porous substrate surfaces for receiving membranes that comprise GPCRs. In one embodiment, a GPCR microarray of the invention comprises a membrane adhered to an upper surface of a porous substrate, the membrane spanning across a plurality of pores on the porous substrate to form a plurality of cavities having sufficient geometry to permit entry of assay reagents into each cavity, thereby allowing access of assay reagents to both sides of GPCR in the membrane.

Abstract: Methods for performing surface-mediated protein delivery into living cells, and fabricating protein-transfected cell cluster arrays are provided. The method comprises providing a protein-containing mixture; depositing said protein-containing mixture onto a surface at defined locations; affixing the protein-containing mixture to the surface as microspots; and plating cells onto the surface in sufficient density and under conditions for the proteins to be delivered into the cells. The protein-containing mixture comprises any suitable amino acid sequence, including peptides, proteins, protein-domains, antibodies, or protein-nucleic acid conjugates, etc., with a carrier reagent. Protein-transfected cell arrays may be used for rapid and direct, screening of protein or enzymatic functions or any given intracellular protein interaction in the natural environment of a living cell, as well as for high-throughput screening of other biological and chemical analytes, which affect the functions of these proteins.

Abstract: A substrate plate or device adapted for use with biological or chemical assays is disclosed. The device may take the form of a multi-well plate having a three- dimensional, porous layer as part of a support surface within each well for immobilizing probe species. The porous layer is characterized as having a plurality of interconnected voids defined by a matrix of contiguous solid material. A method and its variants are also described.

Abstract: Buffered assay solutions for performing 1) binding or 2) functional assays on GPCR arrays, along with methods for their use are described. The buffered assay solution has an underlying composition having: a buffer reagent with a pH in the range of about 6.5 to about 7.9; an inorganic salt of either a monovalent or divalent species, at a concentration from about 1 mM to about 500 mM; and optionally a combination of: c) a blocker reagent at a concentration of about 0.01 wt. % to about 2 wt. % of the composition, or d) protease-inhibitor at a concentration of about 0.001 mM to about 100 mM. In an embodiment for functional assay uses, the composition is modified to also include a GTP-analogue, a guanosine 5?-diphosphate (GDP) salt, and/or an anti-oxidant reagent.

Abstract: The present invention provides a method for the detection of a material comprising ammonium nitrate and a sugar, the method comprising sensing for the presence of hydrogen isocyanate (HNCO). The present invention further provides an apparatus suitable for the examination of a material suspected of comprising ammonium nitrate and a sugar, the apparatus comprising heating means in thermal communication with a sample holder suitable for the containment of the material, the sample holder being in gaseous communication with sensing region, the apparatus being further provided with a means for inducing flow of gas from the sample holder to the sensing region and a means for sensing the presence of hydrogen isocyanate in the sensing region.

Abstract: A thematic microarray and methods for multiplexed binding assays using a cocktail solution of labeled ligands in the presence or absence of a target compound is provided. The methods enables researchers to screening compounds against multiple targets using a microarray format.

Abstract: Screens generated by a host application are reformatted for viewing by applying styles to the host screens in response to recognized components included therein. In particular, a style defines a desired look and layout to be applied to a respective host screen. The styles are applied to the respective host screens based on recognizing components of the host screen at run time. For example, first and second styles can be associated with respective first and second components included in host screens. The first style is applied to the host screen in which the first component is recognized and the second style is applied to the host screen in which the second component is recognized. Accordingly, the application of the first and second styles provide respective first and second reformatted screens.

Abstract: A method of growing semi-insulating GaN epilayers by ammonia-molecular beam epitaxy (MBE) through intentional doping with carbon is described. Thick GaN layers of high resistivity are an important element in GaN based heterostructure field-effect transistors. A methane ion source is preferably used as the carbon dopant source.

Abstract: A semiconductor package (8) with a die (10) having die pads (16) coupled to inner ends (22) of interconnects (20), the die (10) and the interconnects (20) are molded in mold compound (30) with mounting surface (12) and outer ends (24) exposed. A semiconductor die has an interconnect surface opposite the mounting surface.

Abstract: A method of encapsulating organic electroluminescent (EL) devices including providing an EL device on a substrate and a metallic sheet having a stand-off structure depending therefrom, the stand-off structure bounding a cavity larger than the EL device. The stand-off structure is positioned over the EL device with the EL device nested within the cavity. The metallic sheet is adhesively bonded to the substrate at a perimeter outboard of the stand-off structure to seal the organic electroluminescent device in the cavity. When a plurality of EL devices are manufactured on a single substrate, the metallic sheet is perforated at the perimeter of each device so that excess metal can be separated and the substrate separated to provide individual EL devices.

Abstract: A connector affixed to the end of a cable of optical fibers and having alignment pins extending axially in parallel with the cable and beyond the end thereof. A substrate with alignment holes and light transmitting openings therethrough in axial alignment with the ends of the optical fibers when the alignment pins and holes are engaged. The substrate including mounting pads, electrical connection pins and electrical traces connecting the pads to the pins. A semiconductor die, with photonic devices thereon, mounted on the mounting pads of the substrate so that each photonic device is aligned with a light transmitting opening and electrically connected to the electrical connection pins. A sleeve surrounding and securing the connector to the mounting member.

Abstract: A brazed leaded package is provided having buried brazed areas thereby eliminating the need of subsequent protective plating of the brazed areas. The package has a heat conductive support which supports a plurality of leads. The distal end of the leads terminate on the top of the heat conductive support in a braze pad to which are brazed formed leads for providing interface connection to a semiconductor integrated circuit contained within the package. The package can be sealed by using a solid ceramic cover having a glazed glass on its bottom side which is heated up to reflow and provide the seal.