Abstract: A simple, fast, selective and highly sensitive electrochemical method assay and disposable device for detection of viruses, bacteria, proteins, DNA, and/or organic/inorganic compounds. The sensor has a multi-layered construction, with each successive layer performing a different function. The design further allows for the packing of numerous microscopic electrode transducers onto the small footprint of a biochip device, allowing for a high-density array of sensors.