Abstract: The disclosure relates to methods of manufacturing and using a single layer microfluidic for detecting target analytes, including obtaining a single layer sheet of paper; depositing wax boundaries onto the paper in a plurality of patterns including a main channel, fluid transfer channels, and an independent diagnostic area corresponding to each fluid transfer channel; melting the wax through the paper; depositing diagnostic components onto the diagnostic areas; depositing a continuous wax backing; and cutting devices from the paper. The disclosure also relates to a method of capturing an image of the micro fluidic device to generate diagnostic results corresponding to the diagnostic components by: identifying at least two panels from the image; and determining a color for each panel of the at least two panels; and generating for display, using the computing device, a graphical user-interface including at least one component visualizing the diagnostic results.

Abstract: The disclosure relates to methods of manufacturing and using a single layer microfluidic for detecting target analytes, including obtaining a single layer sheet of paper; depositing wax boundaries onto the paper in a plurality of patterns including a main channel, fluid transfer channels, and an independent diagnostic area corresponding to each fluid transfer channel; melting the wax through the paper; depositing diagnostic components onto the diagnostic areas; depositing a continuous wax backing; and cutting devices from the paper. The disclosure also relates to a method of capturing an image of the micro fluidic device to generate diagnostic results corresponding to the diagnostic components by: identifying at least two panels from the image; and determining a color for each panel of the at least two panels; and generating for display, using the computing device, a graphical user-interface including at least one component visualizing the diagnostic results.

Abstract: The disclosure relates to a microfluidic device for detecting and/or quantifying an analyte, wherein the microfluidic device includes a hydrophilic substrate having a main channel, at least two fluid transfer channels fluidically coupled to the main channel, and a separate diagnostic area fluidically coupled to each fluid transfer channel; wax on a surface of the hydrophilic substrate, the wax defining boundaries of the main channel, the at least two fluid transfer channels, and the diagnostic areas; a wax backing on an opposite surface of the hydrophilic substrate; at least one assay reagent provided within at least one of the diagnostic areas; and optionally a calibration region.

Abstract: Disclosed herein a microfluidic device for assessing liver function.