Abstract: The present invention relates to automated devices and methods for the extraction of nucleic acids from cells, the amplification of segments of nucleic acid and the detection of nucleic acids, all in a convenient and portable manner. The invention is particularly suited for use in point-of-care medical diagnostics testing.

Abstract: The present invention relates to automated devices and methods for the extraction of nucleic acids from cells, the amplification of segments of nucleic acid and the detection of nucleic acids, all in a convenient and portable manner. The invention is particularly suited for use in point-of-care medical diagnostic testing.

Abstract: The invention is directed to enzyme immobilization compositions comprising: one or more enzymes, a humectant, an acrylic-based monomer, a water-soluble organic photo-initiator and a water-soluble acrylic-based cross-linker in a substantially homogeneous aqueous mixture. The invention is also directed to methods for forming sensors comprising such compositions and to apparati for forming arrays of immobilized layers on an array of sensors by dispensing such compositions onto a substrate.

Abstract: A composition of matter for use in an immunoassay devices and method comprising a signal antibody, e.g., FAB fragment, covalently linked to a first nucleotide; and one or more signal elements, e.g., signal enzymes such as ALP or fluorescent dyes, each covalently linked to a second nucleotide, wherein the first nucleotide has one or more repeated sequences, and the second nucleotide is bound to one of the one or more repeated sequences on said first nucleotide, and wherein the ratio of the signal antibody to the signal element is controlled by the number of repeated sequences.

Abstract: A BUN (blood urea nitrogen) sensor containing immobilized carbonic anhydrase and immobilized urease for the in vitro detection of urea nitrogen in blood and biological samples with improved performance and precision characteristics.

Abstract: A BUN (blood urea nitrogen) sensor containing immobilized carbonic anhydrase and immobilized urease for the in vitro detection of urea nitrogen in blood and biological samples with improved performance and precision characteristics.

Abstract: The invention is directed to enzyme immobilization compositions comprising: one or more enzymes, a humectant, an acrylic-based monomer, a water-soluble organic photo-initiator and a water-soluble acrylic-based cross-linker in a substantially homogeneous aqueous mixture. The invention is also directed to methods for forming sensors comprising such compositions and to apparati for forming arrays of immobilized layers on an array of sensors by dispensing such compositions onto a substrate.

Abstract: A BUN (blood urea nitrogen) sensor containing immobilized carbonic anhydrase and immobilized urease for the in vitro detection of urea nitrogen in blood and biological samples with improved performance and precision characteristics.

Abstract: A generator that allows for a non-fission based method of producing and recovering 99mTc from neutron-irradiated molybdenum. This generator system is based on the isolation of 99mTc, as the decay product from a source of 99Mo labelled molybdenum carbonyl Mo(CO)6 through a distillation process. The 99mTc obtained from this distillation is produced with high efficiency and purity in a solvent-free form, which can then be dissolved in water or other solvents to produce a solution at the required specific activity and concentration, as reasonably determined by the operator.

Abstract: The present invention relates to automated devices and methods for the amplification of segments of nucleic acid in a convenient and portable manner. A single-use nucleic acid amplification device for producing an amplicon includes a housing and an amplification chamber. The chamber includes an ingress with a first reversible seal, an egress with a second reversible seal, a sealable sample entry orifice, and a first wall forming a portion of the chamber. The first wall includes a thermally conductive material and includes an interior surface and an exterior surface. The exterior surface includes a heating circuit and a temperature sensor. The sample entry orifice permits a sample of nucleic acid to enter the amplification chamber. The ingress is connected to a first conduit along with a pneumatic pump and a fluid pouch. The egress is connected to a second conduit permitting egress of the amplicon from the amplification chamber.

Abstract: The invention provides a steering column assembly for supporting a steering wheel in a vehicle. The steering column assembly includes a steering column having a first end and a longitudinal axis. The steering column assembly is disposable in the vehicle for raking movement about the first end and for collapsing movement along the longitudinal axis. The steering column assembly also includes a collar engaged with the steering column to move with the steering column at least during the raking movement. The steering column assembly also includes a support bracket for supporting at least one gage of the vehicle. The support bracket is releasibly engaged with the collar such that the support bracket separates from the collar in response to the collapsing movement.

Abstract: The invention provides an apparatus and method for absorbing energy in response to a vehicle crash. The apparatus includes a knee bolster disposable in a vehicle for absorbing an impact from at least one knee of a driver. The apparatus also includes a connecting device associated with the knee bolster. The connecting device includes a breakable portion for releasibly connecting the knee bolster to the vehicle such that the knee bolster breaks away from the vehicle under predetermined conditions. The method according to the invention includes the step of disposing a knee bolster in a vehicle for absorbing an impact from at least one knee of a driver. The method also includes the step of releasibly connecting the knee bolster to the vehicle such that the knee bolster breaks away from the vehicle under predetermined conditions with a connecting device associated with the knee bolster and having a breakable portion.

Abstract: A collapsible steering assembly features a steering mechanism and a pedal assemble united and supported together by a support structure as a unitized module for shipping and attachment to a vehicle. The support structure supports the pedal assembly for movement beyond the fully depressed position of one of the pedal therein in response to a predetermined collapse force on the pedal.

Abstract: A collapsible steering assembly features a guide bracket for attachment to a vehicle. A steering mechanism is interconnected with the guide bracket by a steering mechanism support. A plurality of guide rods are arranged about a common collapse axis and extend in non-parallel relationship to a longitudinal axis of the steering mechanism. The guide rods support the steering mechanism for axial movement along the collapse axis in response to a predetermined collapse force on the steering mechanism.

Abstract: The present invention describes a novel business model for performing Research and Development Information Technology Application Software Hosting using remote computing resources. A client computer user is provided access to a state-of-the-art remote computing facility and applications running on servers at this remote computing facility, which are secure, environmentally stabilized and properly managed. The computer user's application is available via a secure data line either through a private Intranet link or using encrypted data over the Internet, using a thin-client type environment. This environment can be expanded wherein access to extremely fast processors can be managed by porting the user environment to a fast processor system employing a means that is essentially transparent to the user. The model provides hardware, software and data product installation, maintenance, support, backup and other services for the client.

Abstract: A printing process determines a maximum pixel printing rate of a printer. An area coverage of an image to be printed is estimated. When the area coverage multiplied by the printing speed exceeds the maximum pixel printing rate, the area coverage of the images to be printed is automatically reduced and the resulting image is printed.

Abstract: A new method for staining biomolecules is described wherein a prepared gelled matrix containing the desired stain is applied onto the biomolecules such that the stain is transferred from the gelled matrix to the biomolecules. This method eliminates the potential hazard of exposing the technician or researcher to the staining solution itself and avoids environmental contamination resulting from the disposal of the staining solution in the water supply.

Abstract: The present invention provides several methods employing nucleic acid repair enzymes. The present invention provides a method for detecting point mutations in nucleic acid sequences. The present invention further provides a method for detecting non-mutated or wild-type nucleic acid sequences. The present invention also enhances target polynucleotide detection using an oscillation reaction and tail labeling techniques, as well as by linking the nucleic acid repair enzyme to a probe molecule. The present invention also provides helix destabilizing molecule and similar molecules to enhance the hybridization of the probe to the target polynucleotides. This invention further provides for the use of thermally stable nucleic acid repair enzymes which will facilitate reactions at elevated temperatures. This invention also provides a method for determining the repair index for a mismatched or damaged oligonucleotide probe.

Abstract: The present invention provides several methods employing nucleic acid repair enzymes. The present invention provides a method for detecting point mutations in nucleic acid sequences. The present invention further provides a method for detecting non-mutated or wild-type nucleic acid sequences. The present invention also enhances target polynucleotide detection using an oscillation reaction and tail labeling techniques. The present invention also provides helix destabilizing molecule and similar molecules to enhance the hybridization of the probe to the target polynucleotides. Finally, this invention provides a method for determining the repair index for a mismatched or damaged oligonucleotide probe.