Abstract: The present invention relates to methods and systems for labeling, isolating, detecting and enumerating biological cells, or other biological analytes of interest present in a sample, where the capturing complex may also serve as a labeling agent. In one embodiment, the capture complex is an encoded magnetic bead coupled to antibodies having a specific affinity for a cell surface protein on a cell of interest. The methods and systems of the present invention can be used for quantitative or qualitative detection.

Abstract: The present invention relates to methods and systems for labeling, isolating, detecting, and/or enumerating a statistically significant number of biological cells, or other biological analytes of interest, present in a complex matrix sample. The isolation of a biological target of interest from a sample mixture is done by immunomagnetic separation. Upon introduction of the sample within an imaging chamber, the capture complex (biological target-magnetic capture agent) will be attracted by the magnetic field and will lay on the surface of the chamber in the focal plane of the imaging system.

Abstract: Systems and methods for performing measurements of one or more materials are provided. One system is configured to transfer one or more materials to an imaging volume of a measurement device from one or more storage vessels. Another system is configured to image one or more materials in an imaging volume of a measurement device. An additional system is configured to substantially immobilize one or more materials in an imaging volume of a measurement device. A further system is configured to transfer one or more materials to an imaging volume of a measurement device from one or more storage vessels, to image the one or more materials in the imaging volume, to substantially immobilize the one or more materials in the imaging volume, or some combination thereof.

Abstract: A method for altering fluorescent emissions of particles includes setting the particles in motion and exposing the moving particles to light such that fluorescent intensities of the particles are lessened isotropically and substantially simultaneously. Another method includes measuring fluorescent emissions of particles, determining the measured fluorescent emissions do not collectively fit within a first predetermined range of fluorescent values, and exposing the particles to one or more incidents of light that are configured to cooperatively alter the fluorescent emissions of the particles to be within a second predetermined range of fluorescent values. An embodiment of an apparatus includes a vessel configured to contain a plurality of particles and a means for setting the particles in motion. The apparatus further includes an illumination subsystem configured to direct light toward the vessel and at a spectral window (i.e.

Abstract: Systems and methods for performing measurements of one or more materials are provided. One system is configured to transfer one or more materials to an imaging volume of a measurement device from one or more storage vessels. Another system is configured to image one or more materials in an imaging volume of a measurement device. An additional system is configured to substantially immobilize one or more materials in an imaging volume of a measurement device. A further system is configured to transfer one or more materials to an imaging volume of a measurement device from one or more storage vessels, to image the one or more materials in the imaging volume, to substantially immobilize the one or more materials in the imaging volume, or some combination thereof.

Abstract: The present invention relates to methods and systems for labeling, isolating, detecting and enumerating biological cells, or other biological analytes of interest present in a sample, where the capturing complex may also serve as a labeling agent. In one embodiment, the capture complex is an encoded magnetic bead coupled to antibodies having a specific affinity for a cell surface protein on a cell of interest. The methods and systems of the present invention can be used for quantitative or qualitative detection.

Abstract: The present invention relates to methods and systems for labeling, isolating, detecting, and/or enumerating a statistically significant number of biological cells, or other biological analytes of interest, present in a complex matrix sample. The isolation of a biological target of interest from a sample mixture is done by immunomagnetic separation. Upon introduction of the sample within an imaging chamber, the capture complex (biological target-magnetic capture agent) will be attracted by the magnetic field and will lay on the surface of the chamber in the focal plane of the imaging system.