Abstract: This invention provides particulates of adenoviral particles comprised of individual adenovirus virions complexed to an insoluble micro-platform material and for such compositions further comprised of a polynucleotide encoding an antigenic peptide. The invention further provides method for forming such complexes such that the compositions are useful for transfecting phagocytic antigen presenting cells such as dendritic cells and for vaccinating a subject against disease.

Abstract: The present invention provides several embodiments that ultimately result in the in vivo loading of endogenous antigenic peptides from a target cell. The invention also presents a method for inducing an immune response to an endogenous antigen in a subject by delivering an effective amount of an agent that stimulates in vivo loading of the endogenous antigen into an Antigenic Peptide Binding Protein (“APBP”). The APBP presents the endogenous antigen to a T cell in vivo. A polynucleotide encoding an APBP is delivered to a target cell under conditions such that the APBP is expressed in the target cell. Endogenous antigenic peptides bind the APBP forming an APBP:peptide complex. A cytotoxic agent also is administered to the subject and delivered to the target cell in an amount effective to lyse the target cell which releases the complexes. The complexes present the antigenic peptide to a T cell or an antigen presenting cell (APC) which mounts the immune response.

Abstract: In order to obtain a novel binding protein against a chosen target, DNA molecules, each encoding a protein comprising one of a family of similar potential binding domains and a structural signal calling for the display of the protein on the outer surface of a chosen bacterial cell, bacterial spore or phage (genetic package) are introduced into a genetic package. The protein is expressed and the potential binding domain is displayed on the outer surface of the package. The cells or viruses bearing the binding domains which recognize the target molecule are isolated and amplified. The successful binding domains are then characterized. One or more of these successful binding domains is used as a model for the design of a new family of potential binding domains, and the process is repeated until a novel binding domain having a desired affinity for the target molecule is obtained.

Abstract: In order to obtain a novel binding protein against a chosen target, DNA molecules, each encoding a protein comprising one of a family of similar potential binding domains and a structural signal calling for the display of the protein on the outer surface of a chosen bacterial cell, bacterial spore or phage (genetic package) are introduced into a genetic package. The protein is expressed and the potential binding domain is displayed on the outer surface of the package. The cells or viruses bearing the binding domains which recognize the target molecule are isolated and amplified. The successful binding domains are then characterized. One or more of these successful binding domains is used as a model for the design of a new family of potential binding domains, and the process is repeated until a novel binding domain having a desired affinity for the target molecule is obtained.

Abstract: In order to obtain a novel binding protein against a chosen target, DNA molecules, each encoding a protein comprising one of a family of similar potential binding domains and a structural signal calling for the display of the protein on the outer surface of a chosen bacterial cell, bacterial spore or phage (genetic package) are introduced into a genetic package. The protein is expressed and the potential binding domain is displayed on the outer surface of the package. The cells or viruses bearing the binding domains which recognize the target molecule are isolated and amplified. The successful binding domains are then characterized. One or more of these successful binding domains is used as a model for the design of a new family of potential binding domains, and the process is repeated until a novel binding domain having a desired affinity for the target molecule is obtained.