Abstract: A method is provided for use in analyzing seeds, in a population of seeds, for one or more desired characteristic. The method includes removing tissue from individual seeds in a population of seeds using an automated seed sampler, while preserving germination viability of the seeds, and analyzing the tissue for the presence or absence of one or more desired characteristic. The method then includes, based on the analysis of the tissue, quantifying the one or more desired characteristic for each of the individual seeds that possess the one or more desired characteristic.

Abstract: A method is provided for use in a plant breeding program to generate a population having one or more desired traits. The method includes removing a tissue sample from each of one or more seeds using an automated seed sampler while preserving germination viability of the sampled seeds; analyzing the tissue samples for the presence or absence of a genetic sequence associated with at least one desired trait; based on the analysis of the tissue samples, cultivating plants from the sampled seeds that either possess or lack the genetic sequence associated with the at least one desired trait; creating a parental cross using at least one of the cultivated plants; and selecting seeds from the parental cross and cultivating offspring of the parental cross using the selected seeds.

Abstract: An automated method for removing tissue from a seed is provided. The automated method includes singulating a seed from a plurality of seeds and imaging the singulated seed to obtain at least one characteristic of the seed. The automated method further includes positioning the singulated seed in a sampler of an automated seed sampling assembly and removing, by the sampler, tissue from the singulated seed based on the at least one characteristic of the seed.

Abstract: A seed sampling system is provided having an automated seed loading assembly including a seed bin and being operable to singulate seeds from a plurality of seeds within the seed bin. The system also includes an automated seed sampling assembly operable to remove tissue samples from the singulated seeds, and an automated seed transport assembly operable to transfer the singulated seeds from the seed loading assembly to the seed sampling assembly. The seed transport assembly includes multiple retention members. Each of the retention members is movable relative to the seed loading assembly and to the seed sampling assembly. The seed transport assembly is operable to position one of the multiple retention members adjacent to the seed loading assembly for engaging one of the singulated seeds, while positioning another of the retention members adjacent to the seed sampling assembly for presenting another of the singulated seeds to the seed sampling assembly.

Abstract: A method is provided for use in a plant breeding program to generate a population having one or more desired traits. The method includes removing a tissue sample from each of one or more seeds using an automated seed sampler while preserving germination viability of the sampled seeds; analyzing the tissue samples for the presence or absence of a genetic sequence associated with at least one desired trait; based on the analysis of the tissue samples, cultivating plants from the sampled seeds that either possess or lack the genetic sequence associated with the at least one desired trait; creating a parental cross using at least one of the cultivated plants; and selecting seeds from the parental cross and cultivating offspring of the parental cross using the selected seeds.

Abstract: Novel methods are provided to facilitate germplasm improvement activities through the use of high throughput, nondestructive sampling of seeds. A method for introgressive hybridization, for example, generally includes removing tissue samples from individual seeds using an automated seed sampler without affecting germination viability of the seeds, and analyzing nucleic acids extracted from the tissue samples for at least one genetic marker. The method then further includes selecting the sampled seeds that possess the at least one genetic marker, cultivating fertile plants from the selected seeds, and crossing the fertile plants with other plants.

Abstract: A seed sampling system is provided having an automated seed loading assembly including a seed bin and being operable to singulate seeds from a plurality of seeds within the seed bin. The system also includes an automated seed sampling assembly operable to remove tissue samples from the singulated seeds, and an automated seed transport assembly operable to transfer the singulated seeds from the seed loading assembly to the seed sampling assembly. The seed transport assembly includes multiple retention members. Each of the retention members is movable relative to the seed loading assembly and to the seed sampling assembly. The seed transport assembly is operable to position one of the multiple retention members adjacent to the seed loading assembly for engaging one of the singulated seeds, while positioning another of the retention members adjacent to the seed sampling assembly for presenting another of the singulated seeds to the seed sampling assembly.

Abstract: Novel methods are provided to facilitate germplasm improvement activities through the use of high throughput, nondestructive sampling of seeds. A method for introgressive hybridization, for example, generally includes removing tissue samples from individual seeds using an automated seed sampler without affecting germination viability of the seeds, and analyzing nucleic acids extracted from the tissue samples for at least one genetic marker. The method then further includes selecting the sampled seeds that possess the at least one genetic marker, cultivating fertile plants from the selected seeds, and crossing the fertile plants with other plants.

Abstract: A seed sampling system is provided having an automated seed loading assembly including a seed bin and being operable to singulate seeds from a plurality of seeds within the seed bin. The system also includes an automated seed sampling assembly operable to remove tissue samples from the singulated seeds, and an automated seed transport assembly operable to transfer the singulated seeds from the seed loading assembly to the seed sampling assembly. The seed transport assembly includes multiple retention members. Each of the retention members is movable relative to the seed loading assembly and to the seed sampling assembly. The seed transport assembly is operable to position one of the multiple retention members adjacent to the seed loading assembly for engaging one of the singulated seeds, while positioning another of the retention members adjacent to the seed sampling assembly for presenting another of the singulated seeds to the seed sampling assembly.

Abstract: The present invention provides for novel methods to facilitate germplasm improvement activities through the use of high throughput, nondestructive sampling of seeds. A method of evaluating seeds for a desired trait, for example, for facilitating germplasm improvement activities, etc., generally includes removing tissue samples from individual seeds using an automated seed sampler without affecting germination viability of the seeds, analyzing the tissue samples for one or more characteristics indicative of a desired trait, and selecting the sampled seeds that include the desired trait.

Abstract: The present invention provides for novel methods to facilitate germplasm improvement activities through the use of high throughput, nondestructive sampling of seeds. A method for analyzing a population of haploid seeds generally includes providing a population of seeds comprising haploid seeds, removing tissue samples from a plurality of the seeds in the population using an automated seed sampler system while preserving the germination viability of the sampled seeds, and analyzing the tissue samples for the presence or absence of one or more characteristics indicative of at least one genetic or chemical trait.

Abstract: A seed sampling system is provided having an automated seed loading assembly including a seed bin and being operable to singulate seeds from a plurality of seeds within the seed bin. The system also includes an automated seed sampling assembly operable to remove tissue samples from the singulated seeds, and an automated seed transport assembly operable to transfer the singulated seeds from the seed loading assembly to the seed sampling assembly. The seed transport assembly includes multiple retention members. Each of the retention members is movable relative to the seed loading assembly and to the seed sampling assembly. The seed transport assembly is operable to position one of the multiple retention members adjacent to the seed loading assembly for engaging one of the singulated seeds, while positioning another of the retention members adjacent to the seed sampling assembly for presenting another of the singulated seeds to the seed sampling assembly.

Abstract: According to the invention, there is provided seed and plants of the corn variety designated CV596775. The invention thus relates to the plants, seeds and tissue cultures of the variety CV596775, and to methods for producing a corn plant produced by crossing a corn plant of variety CV596775 with itself or with another corn plant, such as a plant of another variety. The invention further relates to corn seeds and plants produced by crossing plants of variety CV596775 with plants of another variety, such as another inbred line. The invention further relates to the inbred and hybrid genetic complements of plants of variety CV596775.

Abstract: The present invention provides for novel methods to facilitate germplasm improvement activities through the use of high throughput, nondestructive sampling of seeds. A method for analyzing a population of haploid seeds generally includes providing a population of seeds comprising haploid seeds, removing tissue samples from a plurality of the seeds in the population using an automated seed sampler system while preserving the germination viability of the sampled seeds, and analyzing the tissue samples for the presence or absence of one or more characteristics indicative of at least one genetic or chemical trait.

Abstract: The present invention provides for novel methods to facilitate germplasm improvement activities through the use of high throughput, nondestructive sampling of seeds. A method for analyzing a population of haploid seeds generally includes providing a population of seeds comprising haploid seeds, removing tissue samples from a plurality of the seeds in the population using an automated seed sampler system while preserving the germination viability of the sampled seeds, and analyzing the tissue samples for the presence or absence of one or more characteristics indicative of at least one genetic or chemical trait.

Abstract: The present invention provides for novel methods to facilitate germplasm improvement activities through the use of high throughput, nondestructive sampling of seeds. A method for analyzing a population of haploid seeds generally includes providing a population of seeds comprising haploid seeds, removing tissue samples from a plurality of the seeds in the population using an automated seed sampler system while preserving the germination viability of the sampled seeds, and analyzing the tissue samples for the presence or absence of one or more characteristics indicative of at least one genetic or chemical trait.

Abstract: The present invention provides for novel methods to facilitate germplasm improvement activities through the use of high throughput, nondestructive sampling of seeds. In one embodiment, a high-throughput, non-destructive method for analyzing individual seeds in a population of seeds comprises removing a sample from a plurality of seeds in the population while preserving the germination viability of the seed and analyzing the sample for the presence or absence of one or more characteristics of at least one genetic or chemical trait.

Abstract: According to the invention, there is provided seed and plants of the hybrid corn variety designated CH945822. The invention thus relates to the plants, seeds and tissue cultures of the variety CH945822, and to methods for producing a corn plant produced by crossing a corn plant of variety CH945822 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH945822.

Abstract: According to the invention, there is provided seed and plants of the hybrid corn variety designated CH945822. The invention thus relates to the plants, seeds and tissue cultures of the variety CH945822, and to methods for producing a corn plant produced by crossing a corn plant of variety CH945822 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH945822.

Abstract: According to the invention, there is provided seed and plants of the corn variety designated CV596775. The invention thus relates to the plants, seeds and tissue cultures of the variety CV596775, and to methods for producing a corn plant produced by crossing a corn plant of variety CV596775 with itself or with another corn plant, such as a plant of another variety. The invention further relates to corn seeds and plants produced by crossing plants of variety CV596775 with plants of another variety, such as another inbred line. The invention further relates to the inbred and hybrid genetic complements of plants of variety CV596775.