Patents by Inventor Bruce Seligmann

Bruce Seligmann has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11293048
    Abstract: Methods for detecting nucleic acid sequences, where attenuator oligonucleotides are provided to reduce the number of detection products resulting from highly abundant sequences.
    Type: Grant
    Filed: March 28, 2018
    Date of Patent: April 5, 2022
    Assignee: Bio Spyder Technologies, Inc.
    Inventors: Joanne M. Yeakley, Bruce Seligmann, Joel McComb
  • Patent number: 11268133
    Abstract: Disclosed herein are methods of detecting presence of a gene fusion in a sample from a subject. In some embodiments, the methods of detecting presence of a fusion gene in a sample from a subject utilize a fusion probe that spans the point of fusion between two nucleic acids or genes. In other embodiments, the methods of detecting presence of a fusion gene in a sample from a subject utilize two or more probes that flank the point of fusion between two nucleic acids or genes. In additional embodiments, the methods can include determining the percentage of gene fusion in the sample relative to the first nucleic acid or the second nucleic acid.
    Type: Grant
    Filed: April 8, 2019
    Date of Patent: March 8, 2022
    Assignee: HTG Molecular Diagnostics, Inc.
    Inventors: Bruce A. Seligmann, BJ Kerns, John Luecke, Matt Rounseville, Ihab Botros, Mark Schwartz
  • Patent number: 11091810
    Abstract: Expression profiling of FFPE sample areas smaller than 2 mm2 with single-cell sensitivity, correlated with tissue microenvironment morphology and neoplastic grade. An automated digital molecular pathology instrument for integrated imaging, immunohistochemical assessment, and processing samples for sequence detection assays. Software for instrument and sample control and analysis.
    Type: Grant
    Filed: April 16, 2018
    Date of Patent: August 17, 2021
    Assignee: BioSpyder Technologies, Inc.
    Inventors: Elliot Imler, Milos Babic, Bruce Seligmann
  • Publication number: 20210189466
    Abstract: Methods for designing oligonucleotide probes for detecting and profiling expression products at transcriptome scale.
    Type: Application
    Filed: March 1, 2021
    Publication date: June 24, 2021
    Inventors: Peter J. Shepard, Joanne M. Yeakley, Bruce Seligmann
  • Patent number: 10934576
    Abstract: Oligonucleotides for detecting and profiling expression products at transcriptome scale.
    Type: Grant
    Filed: March 13, 2018
    Date of Patent: March 2, 2021
    Assignee: BioSpyder Technologies, Inc.
    Inventors: Peter J. Shepard, Joanne M. Yeakley, Bruce Seligmann
  • Publication number: 20200385816
    Abstract: Barcoded ligation assay products from individual samples.
    Type: Application
    Filed: May 1, 2020
    Publication date: December 10, 2020
    Inventors: Milos Babic, Christy Lee Trejo, Peter J. Shepard, Joanne M. Yeakley, Bruce Seligmann
  • Patent number: 10683534
    Abstract: Ligation assays in liquid phase for detecting nucleic acid sequences.
    Type: Grant
    Filed: December 22, 2016
    Date of Patent: June 16, 2020
    Assignee: BioSpyder Technologies, Inc.
    Inventors: Anthony Stevens, Bruce Seligmann, Joanne M. Yeakley, Joel McComb
  • Publication number: 20190226013
    Abstract: Disclosed herein are methods of detecting presence of a gene fusion in a sample from a subject. In some embodiments, the methods of detecting presence of a fusion gene in a sample from a subject utilize a fusion probe that spans the point of fusion between two nucleic acids or genes. In other embodiments, the methods of detecting presence of a fusion gene in a sample from a subject utilize two or more probes that flank the point of fusion between two nucleic acids or genes. In additional embodiments, the methods can include determining the percentage of gene fusion in the sample relative to the first nucleic acid or the second nucleic acid.
    Type: Application
    Filed: April 8, 2019
    Publication date: July 25, 2019
    Applicant: HTG Molecular Diagnostics, Inc.
    Inventors: Bruce A. Seligmann, BJ Kerns, John Luecke, Matt Rounseville, Ihab Botros, Mark Schwartz
  • Patent number: 10294515
    Abstract: Disclosed herein are methods of detecting presence of a gene fusion in a sample from a subject. In some embodiments, the methods of detecting presence of a fusion gene in a sample from a subject utilize a fusion probe that spans the point of fusion between two nucleic acids or genes, and detecting the fusion probe after nuclease treatment. In other embodiments, the methods of detecting presence of a fusion gene in a sample from a subject utilize two or more probes that flank the point of fusion between two nucleic acids or genes, and detecting these probes after nuclease treatment. In additional embodiments, the methods can include determining the percentage of gene fusion in the sample relative to the first nucleic acid or the second nucleic acid.
    Type: Grant
    Filed: December 7, 2011
    Date of Patent: May 21, 2019
    Assignee: HTG Molecular Diagnostics, Inc.
    Inventors: Bruce Seligmann, BJ Kerns, John Luecke, Matt Rounseville, Ihab Botros, Mark Schwartz
  • Publication number: 20180237864
    Abstract: Expression profiling of FFPE sample areas smaller than 2 mm2 with single-cell sensitivity, correlated with tissue microenvironment morphology and neoplastic grade. An automated digital molecular pathology instrument for integrated imaging, immunohistochemical assessment, and processing samples for sequence detection assays. Software for instrument and sample control and analysis.
    Type: Application
    Filed: April 16, 2018
    Publication date: August 23, 2018
    Inventors: Elliot Imler, Milos Babic, Bruce Seligmann
  • Publication number: 20180223343
    Abstract: Oligonucleotides for detecting and profiling expression products at transcriptome scale.
    Type: Application
    Filed: March 13, 2018
    Publication date: August 9, 2018
    Inventors: Peter J. Shepard, Joanne M. Yeakley, Bruce Seligmann
  • Publication number: 20180216166
    Abstract: Methods for detecting nucleic acid sequences, where attenuator oligonucleotides are provided to reduce the number of detection products resulting from highly abundant sequences.
    Type: Application
    Filed: March 28, 2018
    Publication date: August 2, 2018
    Inventors: Joanne M. YEAKLEY, Bruce SELIGMANN, Joel McCOMB
  • Patent number: 9957550
    Abstract: Methods for detecting nucleic acid sequences, where attenuator oligonucleotides are provided to reduce the number of detection products resulting from highly abundant sequences.
    Type: Grant
    Filed: September 8, 2014
    Date of Patent: May 1, 2018
    Assignee: BIOSPYDER TECHNOLOGIES, INC.
    Inventors: Joanne M. Yeakley, Bruce Seligmann, Joel McComb
  • Patent number: 9938566
    Abstract: Ligation assays for detecting and profiling expression products at transcriptome scale.
    Type: Grant
    Filed: January 12, 2015
    Date of Patent: April 10, 2018
    Assignee: BioSpyder Technologies, Inc.
    Inventors: Peter J. Shepard, Joanne M. Yeakley, Bruce Seligmann
  • Patent number: 9856521
    Abstract: Ligation assays in liquid phase for detecting nucleic acid sequences.
    Type: Grant
    Filed: June 30, 2015
    Date of Patent: January 2, 2018
    Assignee: BioSpyder Technologies, Inc.
    Inventors: Anthony Stevens, Bruce Seligmann, Joanne M. Yeakley, Joel McComb
  • Patent number: 9765385
    Abstract: Disclosed herein are methods for detecting presence of a nucleotide variant in a target nucleic acid utilizing a nuclease protection assay. The methods include contacting a sample with at least two probes, wherein the first probe is complementary to the wild-type (non-variant) nucleotide(s) at the nucleotide variant position(s) in the target nucleic acid and the second probe is complementary to the variant nucleotide(s) at the nucleotide variant position(s) in the target nucleic acid, under conditions sufficient for the probes to hybridize to the target nucleic acid, producing a mixture of hybridized and unhybridized nucleic acids. The mixture is contacted with a nuclease specific for single-stranded nucleic acid molecules under conditions sufficient to remove unhybridized nucleic acid molecules (or unhybridized portions of nucleic acid molecules). The presence of the at least two probes is then detected, thereby detecting the presence of the variant and/or non-variant target nucleic acid in the sample.
    Type: Grant
    Filed: June 28, 2013
    Date of Patent: September 19, 2017
    Assignee: HTG Molecular Diagnostics, Inc.
    Inventors: Matt Rounseville, Bruce Seligmann
  • Publication number: 20170101671
    Abstract: Ligation assays in liquid phase for detecting nucleic acid sequences.
    Type: Application
    Filed: December 22, 2016
    Publication date: April 13, 2017
    Inventors: Anthony Stevens, Bruce Seligmann, Joanne M. Yeakley, Joel McComb
  • Patent number: 9512469
    Abstract: Disclosed herein are methods of co-detecting presence of target messenger RNA (mRNA) and small non-coding RNA (for example, miRNA) in a sample. The disclosed methods can be used to simultaneously detect mRNA and small non-coding RNA in a single assay (for example in the same reaction or the same well of a multi-well assay). The methods can include contacting a sample with a plurality of nuclease protection probes (NPPs) including at least one probe which specifically binds to a target mRNA and at least one probe which specifically binds to a target small non-coding RNA, contacting the sample with a nuclease specific for single-stranded nucleic acids, and detecting the NPP, for example on a microarray.
    Type: Grant
    Filed: September 26, 2012
    Date of Patent: December 6, 2016
    Assignee: HTG Molecular Diagnostics, Inc.
    Inventors: Bruce Seligmann, Matt Rounseville, Krishna Maddula, Ihab Botros, Chris Cox
  • Publication number: 20160222447
    Abstract: Ligation assays in liquid phase for detecting nucleic acid sequences.
    Type: Application
    Filed: June 30, 2015
    Publication date: August 4, 2016
    Applicant: BIOSPYDER TECHNOLOGIES, INC.
    Inventors: Anthony Stevens, Bruce Seligmann, Joanne M. Yeakley, Joel McComb
  • Publication number: 20160068907
    Abstract: Ligation assays for detecting and profiling expression products at transcriptome scale.
    Type: Application
    Filed: January 12, 2015
    Publication date: March 10, 2016
    Applicant: BioSpyder Technologies, Inc.
    Inventors: Peter J. Shepard, Joanne M. Yeakley, Bruce Seligmann