Abstract: A method and system for extending an extensible framework is provided. An extension module for an extensible framework is coupled with an abstraction layer overlaying the extensible framework. The abstraction layer includes a uniform cross-platform interface between the extension module and the extensible framework.

Abstract: The present invention relates to methods for lyophilizing eukaryotic cells and isolating intact nucleic acids from such cells, and related kits for using the same.

Abstract: An accelerated filesystem includes a fast-path and a slow-path. The fast-path includes an enhanced storage controller and an enhanced network processing function. Uncontested READ and WRITE operations are processed on the fast-path. A READ session is initialized by obtaining file-storage metadata that is tagged with a session ID. The session ID is provided to the enhanced network processing function, and to the application as a file handle, and the tagged metadata is provided to the enhanced storage controller. Subsequent access is facilitated by communicating the file handle from the application to the enhanced network processing function, which passes the file handle to the enhanced storage controller in response. The enhanced storage controller executes a file handle to block list translation by employing the tagged metadata to retrieve the appropriate data. The retrieved data is transmitted to the application via the enhanced network processing function.

Abstract: The present invention is directed to a method for detecting the presence or absence of any specific target nucleic acid sequence contained in a sample. The target sequence can be present in the sample in a relatively pure form or as a component of a member of a mixture of different nucleic acids. The method of the invention utilizes a novel primer design. The sequence of the novel primer is composed of two portions, the 3′ portion is a primer specific for the desired nucleic acid sequence and the 5′ portion is complementary to preselected nucleic acid sequence. Extension of the 3′ portion of the primer with a labeled deoxynucleosides triphosphate yields a labeled extension product if, but only if, the template includes the target sequence. The labeled extension product is detected by hybridization of the 5′ portion to the preselected sequence. The preselected sequence is preferably bound to a solid support as one member of a grid having a group of sequences.

Abstract: A hinge for mounting a door to an enclosure includes a first leaf, a second leaf, and a pin. The first leaf includes a first tongue attached to a surface formed on one of the door or the enclosure, and a first knuckle depending from the first tongue. The first knuckle includes a tab depending therefrom, and the tab extends through an aperture in a flange depending from the surface. The second leaf includes a second tongue coupled to another of the door or the enclosure, and a second knuckle coupled to the second tongue. The pin extends through the first and second knuckles. A method of assembling a hinge to a door includes: extending a portion of the hinge within an aperture in the door; applying a magnetic force to the portion of the hinge to secure the portion of the hinge within the aperture; and securing the hinge to the door with a fastener while applying the magnetic force.

Abstract: The present invention is directed to a method for detecting the presence or absence of any specific target nucleic acid sequence contained in a sample. The target sequence can be present in the sample in a relatively pure form or as a component of a member of a mixture of different nucleic acids. The method of the invention utilizes a novel primer design. The sequence of the novel primer is composed of two portions, the 3′ portion is a primer specific for the desired nucleic acid sequence and the 5′ portion is complementary to preselected nucleic acid sequence. Extension of the 3′ portion of the primer with a labeled deoxynucleosides triphosphate yields a labeled extension product if, but only if, the template includes the target sequence. The labeled extension product is detected by hybridization of the 5′ portion to the preselected sequence. The preselected sequence is preferably bound to a solid support as one member of a grid having a group of sequences.

Abstract: The present invention relates to methods for lyophilizing eukaryotic cells and isolating intact nucleic acids from such cells, and related kits for using the same.

Abstract: A fastener designed to press into two slots of a barrier and become captivated with spring tension barbs is disclosed. The fastener thus allows two vertical barriers to be fastened together by driving a screw through a first barrier, a second barrier, and then the fastener, to compress the barriers in place during assembly. The fastener may be provided with a nut holding cage with caged nut or a pair of thread engaging flanges to secure the screw to the fastener.

Abstract: A door hinge assembly for use in an electrical appliance cabinet includes a door hinge member having a door plate attached to one side of an elbow, a hinge pin sleeve attached to the opposite side of the elbow, and a flange extending upwardly from the elbow. A hinge pin is removably engageable with the hinge pin sleeve, which is further removably engageable with a top edge on the flange.

Abstract: A door hinge assembly for use in an electrical appliance cabinet includes a door hinge member having a door plate attached to one side of an elbow, a hinge pin sleeve attached to the opposite side of the elbow, and a flange extending upwardly from the elbow. A hinge pin is removably engageable with the hinge pin sleeve, which is further removably engageable with a top edge on the flange.

Abstract: The present invention relates to methods for lyophilizing eukaryotic cells and isolating intact nucleic acids from such cells, and related kits for using the same.

Abstract: In an exemplary embodiment of the invention, a machine for shaping a bus bar comprises a first retaining device, a second retaining device, a twisting device and an offset mechanism. The first retaining device is arranged to secure a first section of the bus bar in a first plane. The second retaining device is arranged to secure a second section of the bus bar. The twisting device is connected to the second retaining device and rotates the second retaining device to twist the second section of the bus bar about a longitudinal axis of the bus bar positioning the bus bar in a second plane. The offset mechanism is connected to the first retaining device and is arranged to offset the second section of the bus bar in a third plane, the third plane generally parallel to the second plane.

Abstract: A technique for enabling a shared storage provider (SSP) to provide shared data storage to a plurality of customers while providing greater privacy and security of each customer's data by implementing a virtual private (VP) addressing scheme is disclosed. The technique also allows a storage networking service carrier to provide connectivity between a plurality of customer storage networking sites, keeping each customer separate from each other. In one embodiment, the technique is realized by network architecture and a scheme to separate and virtualize storage traffic and stored data on a shared infrastructure.

Abstract: The extensive synthesis (“amplification”) of a nucleic acid sequence of interest is attained through a linked series of multi-cycle primer extension reactions (LLA). The primers used in each of the primer extension reactions of the process contain non-replicable and/or cleavable elements that halt nucleic acid synthesis and thereby prevent the synthesized molecules from serving as templates in subsequent cycles. Synthesized molecules accumulate during primer extension in a mathematically linear fashion, thereby rendering the process relatively insensitive to contaminating nucleic acids. Multiple primer sets are employed, and simultaneously included in the reaction mixture, thereby ensuring the accumulation of a large number of copies of the nucleic acid sequence of interest. The invention also provides for the detection of an amplified nucleic acid sequence of interest, as well as reagent kits for carrying out the reaction.

Abstract: The present invention performs interpreted remote procedure calls. By utilizing an interpreter, the present invention saves significant storage within a data processing system that performs remote procedure calls. The interpreter of the present invention performs all marshaling and unmarshaling of arguments, thereby consolidating the marshaling and unmarshaling code within a single entity, the interpreter. In performing the marshaling and unmarshaling, the present invention utilizes a descriptor table which contains information pertaining to the argument stack of the remote procedures. By utilizing the present invention, stubs are no longer needed to be used with server side application programs and therefore redundant code and data are minimized throughout the system. However, the present invention also has the flexibility to use stubs with server side application programs where the stubs of the present invention contain a minimal amount of code to interact with the interpreter.

Abstract: A switching module provides connection of a processor to a redundant local area network. Redundant access units switch control selects the access unit used by the processor based on the status of each local area network. In particular, two 10BaseT Ethernet LANs are monitored by scanning for respective link beats or data.

Abstract: The present invention relates to methods for lyophilizing eukaryotic cells and isolating intact nucleic acids from such cells, and related kits for using the same.

Abstract: The extensive synthesis ("amplification") of a nucleic acid sequence of interest is attained through a linked series of multi-cycle primer extension reactions (LLA). The primers used in each of the primer extension reactions of the process contain non-replicable elements that halt nucleic acid synthesis and thereby prevent the synthesized molecules from serving as templates in subsequent cycles. Synthesized molecules accumulate during primer extension in a mathematically linear fashion, thereby rendering the process relatively insensitive to contaminating nucleic acids. Multiple primer sets are employed, thereby ensuring the accumulation of a large number of copies of the nucleic acid sequence of interest. The invention also provides for the detection of an amplified nucleic acid sequence of interest, as well as reagent kits for carrying out the reaction.

Abstract: A process for removing parts-per-million quantities of unwanted species such as oxygen, carbon monoxide, hydrogen, carbon dioxide and water from a cryogenically produced nitrogen gas stream using an oxygen removal step prior to a nickel-based catalytic adsorption step in order to eliminate the need for hydrogen regeneration in either step. Ultrapure nitrogen heated to less than 500.degree. C. is used to purge and hold the beds used for unwanted species removal, eliminating the need for hydrogen regeneration thus reducing the regeneration cycle time and cost.

Abstract: The present invention is directed to a method for detecting the presence or absence of any specific target nucleic acid sequence contained in a sample. The target sequence can be present in the sample in a relatively pure form or as a component of a member of a mixture of different nucleic acids. The method of the invention utilizes a novel primer design. The sequence of the novel primer is composed of two portions, the 3' portion is a primer specific for the desired nucleic acid sequence and the 5' portion is complementary to preselected nucleic acid sequence. Extension of the 3' portion of the primer with a labeled deoxynucleosides triphosphate yields a labeled extension product if, but only if, the template includes the target sequence. The labeled extension product is detected by hybridization of the 5' portion to the preselected sequence. The preselected sequence is preferably bound to a solid support as one member of a grid having a group of sequences.