Patents by Inventor Bruna Marini

Bruna Marini has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • METHODS FOR DNA TARGETS DETECTION DIRECTLY IN CRUDE SAMPLES THROUGH POLYMERASE CHAIN REACTION AND GENOTYPING VIA HIGH RESOLUTION MELTING ANALYSIS
    Publication number: 20210155973
    Abstract: A method id disclosed for DNA targets detection directly in crude samples through Polymerase Chain Reaction (PCR) and genotyping via High Resolution Melting (HRM) analysis. The method comprises diluting the crude sample, treating the diluted crude sample by performing a ramp of increasing and then decreasing temperature, performing PCR amplification and then an HRM analysis. The method further comprises monitoring, during the HRM analysis, the change in the signal emission resulting from the temperature-induced denaturation of the double-stranded amplicons into two single-stranded DNA, due to the release of an intercalating molecule or compound. The detection of DNA targets in the crude sample is performed through a reader analysing the signal variation, obtaining the result of the analysis through a graphic interface connected to the reader.
    Type: Application
    Filed: June 20, 2017
    Publication date: May 27, 2021
    Applicant: ULISSE BIOMED S.P.A.
    Inventors: Rudy Ippodrino, Bruna Marini, Alice Avian, Marco Mocenigo, Nicola Foschi, Elisabetta Mauro
  • MOLECULAR FINGERPRINTING METHODS TO DETECT AND GENOTYPE DNA TARGETS THROUGH POLYMERASE CHAIN REACTION
    Publication number: 20200123598
    Abstract: A molecular fingerprinting method is disclosed to detect and genotype pathogens DNA targets in a sample through polymerase chain reaction (PCR). The method comprises performing PCR amplification and then a High Resolution Melting (HRM) analysis. The PCR reaction mixture used comprises two or more pairs of amplification primers designed in order to generate amplicons with a different melting temperature each other in order to discriminate, in the HRM analysis, each amplicon by observing the specific melting temperature of each amplicon. The method further comprises monitoring, during the HRM analysis, the change in the signal emission resulting from the temperature-induced denaturation of the double-stranded amplicons into two single-stranded DNAs, due to the release of the intercalating molecule or compound.
    Type: Application
    Filed: June 20, 2017
    Publication date: April 23, 2020
    Applicant: ULISSE BIOMED S.P.A.
    Inventors: Rudy Ippodrino, Bruna Marini, Alice Avian, Marco Mocenigo, Nicola Foschi, Elisabetta Mauro, Michele Montrone
  • NUCLEOTIDE-BASED NANOSWITCH AND METHODS FOR THE DETECTION OF ANTIBODIES AND OTHER ANALYTES
    Publication number: 20190153507
    Abstract: A nucleotide-based nanoswitch system for detecting one or more targets in a sample which includes a set of two or more oligonucleotide probes configured to provide a target binding-induced structural change of one or more of the oligonucleotide probes triggered by an increase in local concentration of the two or more oligonucleotide probes.
    Type: Application
    Filed: July 14, 2017
    Publication date: May 23, 2019
    Applicant: ULISSE BIOMED S.R.L.
    Inventors: Rudy Ippodrino, Bruna Marini, Francesco Ricci, Alessandro Porchetta
  • BIOSENSORS FOR THE DETECTION OF INFECTION AND ASSOCIATED MALADIES
    Publication number: 20170212116
    Abstract: The present invention relates to a biosensor for the determination of an infection and possible neoplasias associated with it.
    Type: Application
    Filed: January 26, 2015
    Publication date: July 27, 2017
    Applicant: ULISSE BIOMED SRL
    Inventors: Luca Braga, Bruna Marini, Rudy Ippodrino