Patents by Inventor Bruno DÍEZ GARCÍA

Bruno DÍEZ GARCÍA has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • host cell having improved cellulolytic activity and enzymatic compounds produced with same
    Patent number: 11254956
    Abstract: The invention relates to a host cell, preferably a Myceliophthora thermophila cell, which presents a lower expression and/or secretion of non-contributory cellulolytic enzymes, preferably where the non-contributory cellulolytic enzyme is endoglucanase 6 comprising SEQ ID NO: 2, thereby promoting the presence of contributory cellulolytic enzymes in the enzymatic cocktail synthesised by said host cell. The invention also relates to the use of said host cells and the enzymatic cocktails synthesised by said host cells for the production of fermentable sugars of biomass and a method for producing bioproducts, preferably bioethanol, comprising the use of said host cell or the composition according to the invention.
    Type: Grant
    Filed: May 22, 2017
    Date of Patent: February 22, 2022
    Assignee: ABENGOA BIOENERGÍA NUEVAS TECHNOLOGÍAS, S.A.
    Inventors: Bruno Díez García, Noelia Valbuena Crespo, Francisco Reyes Sosa, Antonio Javier Moreno Pérez, Dolores Pérez Gómez, Ana Isabel Platero Gómez, Lucía Martín Pérez, Sandra Gavaldá Martín, Laura Viñas De La Cruz, Laura Sánchez Zamorano, Consolación Álvarez Núñez, María De Los Ángeles Bermúdez Alcántara, Javier Rocha Martín, Laura Ledesma García, Ricardo Arjona Antolín, Juan Luis Ramos Martín
  • POLYPEPTIDES WITH POLYSACCHARIDE MONOOXYGENASE ACTIVITY AND USE THEREOF FOR THE PRODUCTION OF FERMENTABLE SUGARS
    Publication number: 20200165580
    Abstract: The invention refers to polypeptides with polysaccharide monooxygenase activity, to a host cell that expresses them, preferably a Myceliophthora thermophila cell that recombinantly expresses at least one of these polypeptides, to an enzymatic composition comprising at least one of these polypeptides, preferably together with other cellulolytic enzymes, the use of this host cell, of at least one of the polypeptides with polysaccharide monooxygenase activity or of the enzymatic composition for the degradation of cellulosic biomass and to a process for the production of bioproducts, preferably bioethanol, including the use of this host cell, of at least one of the polypeptides of the invention or of the enzymatic composition of the invention.
    Type: Application
    Filed: January 12, 2018
    Publication date: May 28, 2020
    Inventors: Lucía MARTÍN PÉREZ, Bruno DÍEZ GARCÍA, Francisco Manuel REYES SOSA, Noelia VALBUENA CRESPO, Antonio Javier MORENO PÉREZ, Dolores PÉREZ GÓMEZ, Ana Isabel PLATERO GÓMEZ, Sandra GAVALDÁ MARTÍN, Laura VIÑAS DE LA CRUZ, Javier ROCHA MARTÍN, Laura SÁNCHEZ ZAMORANO, María De Los Angeles BERMÚDEZ ALCÁNTARA, Laura LEDESMA GARCÍA, Juan Luis RAMOS MARTÍN
  • Cellulolytic compositions comprising monooxygenase polysaccharide enzymes with improved activity
    Patent number: 10626381
    Abstract: The invention refers to methods and compositions for stabilizing and increasing the activity of enzymatic mixtures comprising GH61 (PMO or polysaccharide monooxigenase) polypeptides used for the degradation of cellulosic material during the saccharification step of biofuel production processes. This improvement is achieved by the addition of a nickel cation to said enzymatic mixtures before and/or during the saccharification step. Thus, the invention provides compositions comprising PMOs, cellulolytic enzymes and a nickel cation, as well as methods for preparing said compositions and methods for producing fermentable sugars and bioproducts, preferably bioethanol, from cellulosic biomass in which said compositions are used.
    Type: Grant
    Filed: June 23, 2015
    Date of Patent: April 21, 2020
    Assignee: ABENGOA BIOENERGÍA NUEVAS TECNOLOGÍAS, S. A.
    Inventors: Francisco Manuel Reyes Sosa, Bruno Díez García, Noelia Valbuena Crespo, Antonio Javier Moreno Pérez, Dolores Pérez Gómez, Ana Isabel Platero Gómez, Lucía Martín Pérez, Sandra Gavaldá Martín, Laura Viñas De La Cruz, Laura Sánchez Zamorano, Consolación Álvarez Núñez, María de los Angeles Bermúdez Alcántara, Javier Rocha Martín, Laura Ledesma García, Juan Luis Ramos Martín
  • MYCELIOPHTHORA THERMOPHILA HOST CELL HAVING IMPROVED CELLULOLYTIC ACTIVITY AND ENZYMATIC COMPOUNDS PRODUCED WITH SAME
    Publication number: 20190300914
    Abstract: The invention relates to a host cell, preferably a Myceliophthora thermophila cell, which presents a lower expression and/or secretion of non-contributory cellulolytic enzymes, preferably where the non-contributory cellulolytic enzyme is endoglucanase 6 comprising SEQ ID NO: 2, thereby promoting the presence of contributory cellulolytic enzymes in the enzymatic cocktail synthesised by said host cell. The invention also relates to the use of said host cells and the enzymatic cocktails synthesised by said host cells for the production of fermentable sugars of biomass and a method for producing bioproducts, preferably bioethanol, comprising the use of said host cell or the composition according to the invention.
    Type: Application
    Filed: May 22, 2017
    Publication date: October 3, 2019
    Inventors: Bruno DÍEZ GARCÍA, Noelia VALBUENA CRESPO, Francisco REYES SOSA, Antonio Javier MORENO PÉREZ, Dolores PÉREZ GÓMEZ, Ana Isabel PLATERO GÓMEZ, Lucia MARTÍN PÉREZ, Sandra GAVALDÁ MARTÍN, Laura VIÑAS DE LA CRUZ, Laura SÁNCHEZ ZAMORANO, Consolación ÁLVAREZ NÚÑEZ, María De LosÁngeles BERMÚDEZ ALCÁNTRARA, Javier ROCHA MARTÍN, Laura LEDESMA GARCÍA, Ricardo ARJONA ANTOLÍN, Juan Luis RAMOS MARTÍN
  • Variants of cellobiohydrolase 1
    Patent number: 10385324
    Abstract: The present invention relates to variants of cellobiohydrolase, preferably Cbh1, which have greater cellobiohydrolase activity. The invention also relates to a genetic construct, a host cell and to an enzyme composition comprising said variants. The invention further relates to a procedure for producing fermentable sugar and a procedure for producing a bioproduct, such as bioethanol, from cellulose material with the cellobiohydrolase variants, the host cell or the enzyme composition comprising said variants.
    Type: Grant
    Filed: April 8, 2016
    Date of Patent: August 20, 2019
    Assignee: ABENGOA BIOENERGÍA NUEVAS TECNOLOGÍAS, S.A.
    Inventors: Bruno Díez García, Noelia Valbuena Crespo, Francisco Manuel Reyes Sosa, Antonio Javier Moreno Pérez, Dolores Pérez Gómez, Ana Isabel Platero Gómez, Lucia Martín Pérez, Sandra Gavaldá Martín, Laura Viñas De La Cruz, Laura Sánchez Zamorano, Consolación Álvarez Núñez, María de los Ángeles Bermúdez Alcántara, Javier Rocha Martín, Laura Ledesma García, Juan Luis Ramos Martín, Laura Benítez Casanova, Macarena López Morales
  • EXPRESSION OF RECOMBINANT BETA-XYLOSIDASE ENZYMES
    Publication number: 20180230446
    Abstract: The present invention relates to a Myceliophthora thermophila cell, which expresses a nucleotide sequence that codifies a recombinant beta-xylosidase enzyme comprising an amino-acid sequence having at least 70% identity with SEQ ID NO: 1, an enzymatic composition comprising said cell and/or the recombinant enzyme with beta-xylosidase activity expressed by said cell, the use of this host cell, the recombinant enzyme with beta-xylosidase activity expressed by said cell or the composition for the degradation of biomass, and a method of producing biological products, preferably bioethanol, comprising the use of said host cell, the recombinant enzyme with the beta-xylosidase activity expressed by said cell or said composition.
    Type: Application
    Filed: July 29, 2015
    Publication date: August 16, 2018
    Inventors: Bruno DÍEZ GARCÍA, Noelia VALBUENA CRESPO, Francisco Manuel REYES SOSA, Antonio Javier MORENO PÉREZ, Dolores PÉREZ GÓMEZ, Lucía MARTÍN PÉREZ, Sandra GAVALDÁ MARTÍN, Ana Isabel PLATERO GÓMEZ, Laura í VINAS DE LA CRUZ, Laura SÁNCHEZ ZAMORANO, Consolación ÁLVAREZ NÚNEZ, María de los Ángeles BERMÚDEZ ALCANTARA, Laura LEDESMA GARCÍA, Javier ROCHA MARTÍN, Juan Luis RAMOS MARTÍN, Laura BENÍTEZ CASANOVA, Macarena LÓPEZ MORALES
  • CELLULOLYTIC COMPOSITIONS COMPRISING MONOOXYGENASE POLYSACCHARIDE ENZYMES WITH IMPROVED ACTIVITY
    Publication number: 20180187167
    Abstract: The invention refers to methods and compositions for stabilizing and increasing the activity of enzymatic mixtures comprising GH61 (PMO or polysaccharide monooxigenase) polypeptides used for the degradation of cellulosic material during the saccharification step of biofuel production processes. This improvement is achieved by the addition of a nickel cation to said enzymatic mixtures before and/or during the saccharification step. Thus, the invention provides compositions comprising PMOs, cellulolytic enzymes and a nickel cation, as well as methods for preparing said compositions and methods for producing fermentable sugars and bioproducts, preferably bioethanol, from cellulosic biomass in which said compositions are used.
    Type: Application
    Filed: June 23, 2015
    Publication date: July 5, 2018
    Inventors: Francisco Manuel REYES SOSA, Bruno DÍEZ GARCÍA, Noelia VALBUENA CRESPO, Antonio Javier MORENO PÉREZ, Dolores PÉREZ GÓMEZ, Ana Isabel PLATERO GÓMEZ, Lucía MARTÍN PÉREZ, Sandra GAVALDÁ MARTÍN, Laura VIÑAS DE LA CRUZ, Laura SÁNCHEZ ZAMORANO, Consolación ÁLVAREZ NÚÑEZ, María de los Angeles BERMÚDEZ ALCÁNTARA, Javier ROCHA MARTÍN, Laura LEDESMA GARCÍA, Juan Luis RAMOS MARTÍN
  • IMPROVED VARIANTS OF CELLOBIOHYDROLASE 1
    Publication number: 20180142225
    Abstract: The present invention relates to variants of cellobiohydrolase, preferably Cbh1, which have greater cellobiohydrolase activity. The invention also relates to a genetic construct, a host cell and to an enzyme composition comprising said variants. The invention further relates to a procedure for producing fermentable sugar and a procedure for producing a bioproduct, such as bioethanol, from cellulose material with the cellobiohydrolase variants, the host cell or the enzyme composition comprising said variants.
    Type: Application
    Filed: April 8, 2016
    Publication date: May 24, 2018
    Inventors: Bruno DÍEZ GARCÍA, Noelia VALBUENA CRESPO, Francisco Manuel REYES SOSA, Antonio Javier MORENO PÉREZ, Dolores PÉREZ GÓMEZ, Ana Isabel PLATERO GÓMEZ, Lucía MARTÍN PÉREZ, Sandra GAVALDÁ MARTÍN, Laura VIÑAS DE LA CRUZ, Laura SÁNCHEZ ZAMORANO, Consolación ÁLVAREZ NÚÑEZ, María de los Ángeles BERMÚDEZ ALCÁNTARA, Javier ROCHA MARTÍN, Laura LEDESMA GARCÍA, Juan Luis RAMOS MARTÍN, Laura BENÍTEZ CASANOVA, Macarena LÓPEZ MORALES
  • Expression of recombinant beta-xylosidase enzymes
    Patent number: 9834763
    Abstract: The present invention relates to a Myceliophthora thermophila host cell which expresses a recombinant enzymes from Fusarium oxysporum with beta-xylosidase activity. The invention also refers to an enzymatic composition comprising the host cell of the invention and/or the recombinant enzyme with beta-xylosidase activity expressed by the host cell of the invention. The invention further relates to the use of the host cell of the invention, the recombinant enzyme with beta-xylosidase activity expressed by the host cell of the invention or the composition of the invention for the degradation of biomass and to a method of producing bioproducts, preferably bioethanol, which comprises the use of the host cell of the invention, the recombinant enzyme with beta-xylosidase activity expressed by the host cell of the invention or the composition of the invention.
    Type: Grant
    Filed: May 20, 2013
    Date of Patent: December 5, 2017
    Assignee: Abengoa Bioenergia Nuevas Tecnologias, S.A.
    Inventors: Bruno Diez Garcia, Ana Gomez Rodriguez, Jorge Gil Martinez, Noelia Valbuena Crespo, Antonio Javier Moreno Perez, Rafael Duenas Sanchez, Ana Maria Munoz Gonzalez, Dolores Perez Gomez, Lucia Martin Perez, Sandra Gavalda Martin, Laura Sanchez Zamorano, Consolacion Alvarez Nunez, Maria de los Angeles Bermudez Alcantara, Pablo Gutierrez Gomez, Ricardo Arjona Antolin
  • Expression of Recombinant Beta-Xylosidase Enzymes
    Publication number: 20160272956
    Abstract: The present invention relates to a host cell, preferably Myceliophthora thermophila cell, which expresses recombinant enzymes with beta-xylosidase activity, an enzymatic composition comprising said cell and/or the recombinant enzyme with beta-xylosidase activity expressed by said cell, the use of this host cell, the recombinant enzyme with beta-xylosidase activity expressed by said cell or the composition for the degradation of biomass and a method of producing bioproducts, preferably bioethanol, which comprises the use of said host cell, the recombinant enzyme with beta-xylosidase activity expressed by said cell or said composition.
    Type: Application
    Filed: May 20, 2013
    Publication date: September 22, 2016
    Inventors: Bruno Diez Garcia, Ana Gomez Rodriguez, Jorge Gil Marintez, Noelia Valbuena Crespo, Antonio Javier Moreno Perez, Rafael Duenas Sanchez, Ana Maria Munoz Gonzalez, Dolores Perez Gomez, Lucia Martin Perez, Sandra Gavalda Martin, Laura Sanchez Zamorano, Consolacion Alvarez Nunez, Maria de los Angeles Bermudez Alcantara, Pablo Gutierrez Gomez, Ricardo Arjona Antolin
  • Method of producing lycopene through the fermentation of selected strains of , formulations and uses of the lycopene thus obtained
    Patent number: 7799540
    Abstract: The method of fermentation with selected strains of B. trispora described in the present invention makes it possible to achieve lycopene yields higher than those currently described. The methods of isolation, purification and formulation are applicable to any natural source of lycopene, especially to submerged cultures of mucoral fungi of the genera Blakeslea, Choanephora, Phycomyces or Mucor. The method of extraction makes it possible to simplify the recovery process and increase the purity of the product, relative to the methods previously described. The methods of formulation provide high added value, since they make it possible to obtain stabilized preparations of lycopene for direct application in the food and pharmaceutical fields.
    Type: Grant
    Filed: December 20, 2002
    Date of Patent: September 21, 2010
    Assignee: Vitatene, S.A.
    Inventors: Ana Teresa Marcos Rodríguez, Antonio Estrella De Castro, Javier Costa Perez, Manuel Antonio Oliver Ruiz, Nieves Fraile Yecora, Juan Luis De La Fuente Moreno, Marta Rodríguez Saiz, Bruno Diez Garcia, Enrieque Peiro Cezon, Angel Muñoz Ruiz, Walter Cabri, Juan Francisco Lopez Ortiz, José Luis Barredo Fuente
  • Method of production of ?-carotene by fermentation in mixed culture using (+) and (?) strains of
    Patent number: 7252965
    Abstract: The invention consists of fermenting selected strains of Blakeslea trispora in conditions such that ?-carotene is produced in the form of stabilized preparations with residual contents of other carotenoids (?-carotene and ?-zeacarotene). The fermentation conditions chosen include the programmed addition of oxygen and/or ?-ionone during fermentation, and control of the age of the vegetative growth phases of the strains employed. The ?-carotene obtained can be used in the pharmaceutical and food sectors.
    Type: Grant
    Filed: January 23, 2003
    Date of Patent: August 7, 2007
    Assignee: Antibioticos, S.A.U.
    Inventors: Javier Costa Perez, Ana Teresa Marcos Rodríguez, Juan Luis De La Fuente Moreno, Marta Rodríguez Saiz, Bruno Diez Garcia, Enrique Peiro Cezon, Walter Cabri, José Luis Barredo Fuente
  • Biosyntheticse genes of blakeslea trispora beta-carotene that code for lycopene cyclase/phytoene synthase (carrp) and phytoene dehydrogenase (carb)
    Publication number: 20050124031
    Abstract: Biosynthetic genes of ?-carotene from Blakeslea trispora that code for lycopene cyclase/phytoene synthase (carRP) and phytoene dehydrogenase (carB). The carRP gene, characterized by the DNA sequence SEQ ID NO:1, codes for the amino acid sequence SEQ ID NO:3, which has the lycopene cyclase/phytoene synthase enzymes. For its part, the carB gene, characterized by the DNA sequence SEQ ID NO:2, codes for the amino acid sequence SEQ ID NO:4, which possesses phytoene dehydrogenase enzyme activity. In addition, (i) procedures for constructing plasmids for the expression of additional copies of the aforesaid genes and (ii) methods for the expression of heterologous genes under the control of the promoters of said genes, are described.
    Type: Application
    Filed: September 26, 2002
    Publication date: June 9, 2005
    Applicant: VITATENE, S.A.
    Inventors: Marta Rodriguez Saiz, Ana Marcos Rodriguez, Bruno Diez Garcia, Juan Dela Fuente Moreno, Jose Barredo Fuente
  • Method of production of astaxanthin by fermenting selected strains of xanthophyllomyces dendrorhous
    Publication number: 20050124032
    Abstract: The present invention describes methods for (i) obtaining astaxanthin-overproducing strains of X. dendrorhous and (ii) the use of the aforementioned strains in improved conditions of fermentation. The methods used for selecting the strains are based on: (i) resistance to inhibitors of steroid synthesis, to inhibitors of respiration and to compounds that induce the formation of free radicals, (ii) color intensity of the colony and production of carotenoids on solid medium, (iii) production of astaxanthin in darkness, (iv) production of astaxanthin in conditions with a raised temperature and (v) production of astaxanthin with carbon sources other than glucose. These methods permit the efficient selection of mutants of X. dendrorhous with astaxanthin yields higher than the parent strain and little accumulation of other carotenoids. Cultivation of X. dendrorhous in liquid medium makes it possible to obtain a biomass that can be used directly in human and animal nutrition.
    Type: Application
    Filed: February 3, 2003
    Publication date: June 9, 2005
    Inventors: Juan De La Fuente Moreno, Enrique Peiro Cezon, Bruno Diez Garcia, Ana Marcos Rodriguez, Carmen Schleissner Sanchez, Marta Rodriguez Saiz, Carmelita Rodriguez Otero, Walter Cabri, Jose Barredo Fuente
  • Method of producing lycopene through the fermentation of selected strains of blackeslea trispora, formulations and uses of the lycopene thus obtained
    Publication number: 20050106657
    Abstract: The method of fermentation with selected strains of B. trispora described in the present invention makes it possible to achieve lycopene yields higher than those currently described. The methods of isolation, purification and formulation are applicable to any natural source of lycopene, especially to submerged cultures of mucoral fungi of the genera Blakeslea, Choanephora, Phycomyces or Mucor. The method of extraction makes it possible to simplify the recovery process and increase the purity of the product, relative to the methods previously described. The methods of formulation provide high added value, since they make it possible to obtain stabilized preparations of lycopene for direct application in the food and pharmaceutical fields.
    Type: Application
    Filed: December 20, 2002
    Publication date: May 19, 2005
    Inventors: Ana Marcos Rodriguez, Antonio Estrella De Castro, Javier Costa Perez, Manuel Oliver Ruiz, Nieves Fraile Yecora, Juan De La Fuente Moreno, Marta Saiz, Bruno Diez Garcia, Enrieque Peiro Cezon, Angel Ruiz, Walter Cabri, Juan Lopez Ortiz, Jose Barredo Fuente
  • Gene from acremonium chrysogenum encoding a protein with cephalosporin C acetylhydrolase activity and methods of use of such gene
    Patent number: 6815189
    Abstract: An isolated DNA sequence that encodes a peptide with CPC-acetylhydrolase enzymatic activity is disclosed. Also, a method of expressing CPC-acetylhydrolase activity that includes the steps of (a) providing a microorganism that is susceptible to transformation with the isolated DNA sequence and that, upon transformation, expresses the CPC-acetylhydrolase activity encoded by the sequence, and (b) transforming the microorganism with the DNA sequence to cause expression of the CPC-acetylhydrolase activity in the microorganism is disclosed. Also, a method for producing a microorganism with increased capacity to aid in the production of cephalosporin, which method includes the steps of (a) providing a microorganism that has CPC-AH activity by virtue of expression of the DNA sequence or of a fragment thereof coding for the CPC-acetylhydrolase enzyme activity; and (b) inactivating the activity by disrupting expression of the DNA sequence is disclosed.
    Type: Grant
    Filed: March 1, 2002
    Date of Patent: November 9, 2004
    Assignee: Antibiotics, S.A.U.
    Inventors: Javier Velasco Alvarez, Santiago Gutierrez Martin, Francisco Javier Casqueiro Blanco, Sonia Campoy Garcia, Francisco Fierro Fierro, Jose Luis Barredo Fuente, Bruno Diez Garcia, Juan Francisco Martin Martin
  • Method of producing $g(b)-carotene by means of mixed culture fermentation using (+) and () strains of blakeslea trispora
    Publication number: 20040152154
    Abstract: Method of production of &bgr;-carotene by fermentation in mixed culture using (+) and (−) strains of Blakeslea trispora
    Type: Application
    Filed: January 21, 2004
    Publication date: August 5, 2004
    Inventors: Javier Costa Perez, Ana Teresa Marcos Rodriguez, Juan Luis De La Fuente Moreno, Marta Rodriguez Saiz, Bruno Diez Garcia, Enrique Peiro Cezon, Walter Cabri, Jose Luis Barredo Fuente
  • Enzymatic process for the preparation of cephalosporanic 7$B(G)-(4-Carboxybutanamide) acid by means of the modified enzyme D-aminoacid oxidase of trigonopsis variabilis produced in escherichia coli
    Patent number: 6635458
    Abstract: Enzymatic process for the preparation of cephalosporanic 7&bgr;-(4-carboxybutanamide) acid by using the modified enzyme D-aminoacid oxidase of Trigonopsis variabilis produced in Escherichia coli. The process for the expression of the enzyme comprises: (I) isolating the DNA corresponding to gene which codes for the enzyme D-aminoacid oxidase; (II) removing the intron which is contained in said gene; (III) inserting the DNA fragment obtained into the plasmide which is capable of replication in Escherichia coli; (IV) fusing at the extremity 5′ of the structural region of the gene a synthetic assembler which contains a nucleotide sequence which codes for six histidines; (V) transforming a strain of Escherichia coli with the resulting recombinant plasmide; (VI) cultivating the transformed cells of Escherichia coli; and (VII) recovering the enzyme D-aminoacid oxidase of the former cultivation operation through affinity chromatography.
    Type: Grant
    Filed: July 15, 1999
    Date of Patent: October 21, 2003
    Assignee: Antibioticos, S.A.U.
    Inventors: Jose Luis Garcia Lopez, Estrella Cortes Rubio, Jorge Alonso Palacios, Encarnacion Mellado Duran, Bruno Diez Garcia, Jose Manuel Guisan Seijas, Franciso Salto Maldonado, Barredo Fuente
  • ENZYMATIC PROCESS FOR THE PREPARATION OF CEPHALOSPORANIC 7$B(G)-(4-CARBOXYBUTANAMIDE) ACID BY MEANS OF THE MODIFIED ENZYME D-AMINOACID OXIDASE OF TRIGONOPSIS VARIABILIS PRODUCED IN ESCHERICHIA COLI
    Publication number: 20030119087
    Abstract: Enzymatic process for the preparation of cephalosporanic 7?-(4-carboxybutananude) acid by using the modified enzyme D-aminoacid oxidase of Trigonopsis variabilis produced in Escherichia coli. The process for the expression of the enzyme comprises: (I) isolating the DNA corresponding to gene which codes for the enzyme D-aminoacid oxidase; (II) removing the intron which is contained in said gene; (III) inserting the DNA fragment obtained into the plasmide which is capable of replication in Escherichia coli; (IV) fusing at the extremity 5′ of the structural region of the gene a synthetic assembler which contains a nucleotide sequence which codes for six histidines; (V) transforming a strain of Escherichia coli with the resulting recombinant plasmide; (VI) cultivating the transformed cells of Escherichia coli; and (VII) recovering the enzyme D-aminoacid oxidase of the former cultivation operation through affinity chromatography.
    Type: Application
    Filed: July 15, 1999
    Publication date: June 26, 2003
    Inventors: JOSE LUIS GARCIA LOPEZ, ESTRELLA CORTES RUBIO, JORGE ALONSO PALACIOS, ENCARNACION MELLADO DURAN, BRUNO DIEZ GARCIA, JOSE MANUEL GUISAN SEIJAS, FRANCISO SALTO MALDONADO, BARREDO FUENTE
  • Promoters of the genes glutamate deshydrogenase, &bgr;-N-acetylhexosaminidase and &ggr;-actin and their use in filamentous fungi expression, secretion and antisense systems
    Patent number: 6558921
    Abstract: An isolated DNA having the promoter sequence of the hex gene of P. chrysogenum or a DNA fragment that is hybridizable to the complement of the promoter sequence under stringent conditions and is capable of directing expression of DNA downstream of the fragment in P. chrysogenum. Also a process for promoting expression of a coding sequence of interest in a microorganism using the isolated DNA and a process to block expression of a gene of interest in a microorganism using the isolated DNA are disclosed.
    Type: Grant
    Filed: August 2, 2000
    Date of Patent: May 6, 2003
    Assignee: Antibioticos, S.A.
    Inventors: Jose Luis Barredo Fuente, Marta Rodriguez Saiz, Alfonso J. Collados de la Vieja, Migeul Angel Moreno Valle, Francisco Salto Maldonado, Bruno Diez Garcia