Abstract: A method for simultaneously merging suspended particles and/or cells in a plurality of discrete microwells, each of said microwells having at least a bottom wall and lateral walls, wherein a multifrequency or broadband acoustic wave including at least two different frequencies is applied to an inner volume of each of said microwells, the frequencies of said acoustic wave being selected to generate a standing and/or stationary wave in said volume; a device for simultaneously merging suspended particles and/or cells in a plurality of discrete microwells, including: a substrate with a plurality of discrete microwells, each of said microwells having at least a bottom wall and lateral walls; and one or more acoustic transducer(s), configured for applying a multifrequency or broadband acoustic wave, including at least two different frequencies, to an inner volume of each of said microwells.

Abstract: A system and a methodology are provided with a broad range of application in immune diagnostic screening and therapy and specifically for predicting the risk of graft versus host disease (GVHD) and/or graft versus leukemia (GVL) effects prior to transplantation. The method includes the steps of incubating single T or NK cells with a few, usually three to five target cells for extended period of times and evaluating the cell contact-dependent lytic activity or activation of the single effector cells within larger populations. The results obtained are analyzed by proprietary software for automated image analysis and compared with patient data comprised in a comprehensive database containing accumulated empirical and clinical information on donor-recipient screening results and patient information.