Patents by Inventor Bruno Winter
Bruno Winter has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9637728Abstract: The present invention provides T. reesei endoglucanase I variants exhibiting improved cellulase and/or xylanase activity, in particular at 30° C. and/or pH 6. These variants are particularly useful in industrial processes comprising simultaneous degradation of cellulosic biomass into monomeric sugars and fermentation of said sugars, for example to produce ethanol.Type: GrantFiled: July 25, 2014Date of Patent: May 2, 2017Assignee: BIOMETHODESInventors: Stéphane Blesa, Céline Coursange, Bruno Winter
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Publication number: 20160208232Abstract: The present invention provides T. reesei endoglucanase I variants exhibiting improved cellulase and/or xylanase activity, in particular at 30° C. and/or pH 6. These variants are particularly useful in industrial processes comprising simultaneous degradation of cellulosic biomass into monomeric sugars and fermentation of said sugars, for example to produce ethanol.Type: ApplicationFiled: July 25, 2014Publication date: July 21, 2016Inventors: STÉPHANE BLESA, CÉLINE COURSANGE, BRUNO WINTER
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Patent number: 9353357Abstract: The invention relates to a recombinant DNA molecule encoding a polypeptide having phytase activity and increased temperature stability and increased proteolytic stability of the enzyme activity. The DNA sequence has been obtained by variation of the mature wild-type E. coli phytase sequence with defined amino acid positions being modified in comparison to the wild-type sequence or with the sequences having N- and/or C-terminal extensions, respectively. The invention further relates to a method for expressing the recombinant phytase as well as its use in the food and animal feed technologies.Type: GrantFiled: March 18, 2013Date of Patent: May 31, 2016Assignee: AB Enzymes GmbHInventors: Khanh Q. Nguyen, Bruno Winter
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Patent number: 9109210Abstract: The present invention provides the field of enhancing proteins and in particular to that of proteins enhanced by molecular change. It provides a variant of a phytase that is termed enhanced in that is has better thermostability and/or activity than the original phytase. The invention also provides a nucleic acid coding for said variant, a cassette or an expression vector containing said variant, a host cell expressing said variant, a composition comprising said variant and uses thereof, principally in the preparation of food additives and animal feed.Type: GrantFiled: July 1, 2010Date of Patent: August 18, 2015Assignee: BIOMETHODESInventors: Stephane Blesa, Helene Chautard, Marc Delcourt, Laurent Mesta, Bruno Winter
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Patent number: 8822201Abstract: The invention relates to a method for recovering of oil from plant seeds characterized in that a) an aqueous solution containing one or more cellulolytic and/or lipolytic and/or pectinolytic and/or proteolytic enzyme(s) and/or phytase is sprayed onto the seed, b) the thus obtained seed is directly supplied to a one-stage or multistage pressing in a way known per se, optionally coupled to an extraction, and c) the oil is recovered in a way known per se and optionally further processed, and the use of the method, particularly in the production of edible oil or biodiesel.Type: GrantFiled: December 5, 2007Date of Patent: September 2, 2014Assignee: AB Enzymes GmbHInventors: Jörg Köhler, Volker Marschner, Bruno Winter
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Publication number: 20140242249Abstract: The invention relates to a recombinant DNA molecule encoding a polypeptide having phytase activity and increased temperature stability and increased proteolytic stability of the enzyme activity. The DNA sequence has been obtained by variation of the mature wild-type E. coli phytase sequence with defined amino acid positions being modified in comparison to the wild-type sequence or with the sequences having N- and/or C-terminal extensions, respectively. The invention further relates to a method for expressing the recombinant phytase as well as its use in the food and animal feed technologies.Type: ApplicationFiled: March 18, 2013Publication date: August 28, 2014Applicant: AB ENZYMES GMBHInventors: Khanh Q. Nguyen, Bruno Winter
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Patent number: 8653241Abstract: The invention relates to a DNA sequence that encodes a polypeptide with phospholipase activity and was isolated from Aspergillus and sequences derived therefrom, polypeptides with phospholipase activity encoded by these sequences as well as the use of these polypeptides for degumming of vegetable oil, for the preparation of dough and/or bakery products, for the preparation of dairy products, for processing steps in the textile industry and for related applications.Type: GrantFiled: June 28, 2011Date of Patent: February 18, 2014Assignee: AB Enzymes GmbHInventors: Khanh Q. Nguyen, Volker Marschner, Kornelia Titze, Bruno Winter
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Patent number: 8507241Abstract: The invention relates to a DNA sequence that encodes a polypeptide with lysophospholipase activity and was isolated from Aspergillus and sequences derived therefrom, polypeptides with lysophospholipase activity encoded by these sequences as well as the use of these polypeptides for improving the filterability of syrups consisting of wheat starch and for related applications.Type: GrantFiled: May 10, 2012Date of Patent: August 13, 2013Assignee: AB Enzymes GmbHInventors: Khanh Q. Nguyen, Volker Marschner, Kornelia Titze, Bruno Winter
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Patent number: 8465946Abstract: The present invention relates to a microbial expression system for the production of polypeptides based on the use of extrachromosomal DNA, whereby no antibiotic marker genes for the selection of the host cell but DNA sequences that encode glycerine-3-phosphate dehydrogenase are used, and, thus, the production of the desired polypeptide, e.g., xylanase, does not need the addition of antibiotics. The expression system is free from antibiotic-resistance genes. The invention further relates to a DNA sequence that encodes a polypeptide with glycerine-3-phosphate dehydrogenase activity as well as a polypeptide with glycerine-3-phosphate dehydrogenase activity.Type: GrantFiled: February 12, 2008Date of Patent: June 18, 2013Assignee: AB Enzymes GmbHInventors: Céline Cadot, Tina Ploss, Ruth Schwerdtfeger, Bruno Winter
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Publication number: 20130122567Abstract: The present invention provides the field of enhancing proteins and in particular to that of proteins enhanced by molecular change. It provides a variant of a phytase that is termed enhanced in that is has better thermostability and/or activity than the original phytase. The invention also provides a nucleic acid coding for said variant, a cassette or an expression vector containing said variant, a host cell expressing said variant, a composition comprising said variant and uses thereof, principally in the preparation of food additives and animal feed.Type: ApplicationFiled: July 1, 2010Publication date: May 16, 2013Applicant: BIOMETHODESInventors: Stephane Blesa, Helene Chautard, Marc Delcourt, Laurent Mesta, Bruno Winter
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Patent number: 8420369Abstract: The invention relates to a recombinant DNA molecule encoding a polypeptide having phytase activity and increased temperature stability and increased proteolytic stability of the enzyme activity. The DNA sequence has been obtained by variation of the mature wild-type E. coli phytase sequence with defined amino acid positions being modified in comparison to the wild-type sequence or with the sequences having N- and/or C-terminal extensions, respectively. The invention further relates to a method for expressing the recombinant phytase as well as its use in the food and animal feed technologies.Type: GrantFiled: November 2, 2007Date of Patent: April 16, 2013Assignee: AB Enzymes GmbHInventors: Khanh Q. Nguyen, Bruno Winter
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Patent number: 8313937Abstract: The present invention features a recombinant DNA molecule which, upon expression in a prokaryotic or eukaryotic host cell, encodes a polypeptide having phytase activity. In preferred embodiments, the recombinant DNA molecule comprises a DNA sequence selected from DNA sequences which have been obtained by variations of the mature wild-type E. coli phytase sequence, wherein at least one amino acid at position 200 or position 207 is mutated as compared to the wild-type sequence, where the recombinant DNA molecule is, upon expression in a suitable host cell, associated with an increased activity of the thus encoded protein in the culture supernatant.Type: GrantFiled: October 14, 2005Date of Patent: November 20, 2012Assignee: AB Enzymes GmbHInventors: Khanh Q. Nguyen, Bruno Winter
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Publication number: 20120219666Abstract: The invention relates to a DNA sequence that encodes a polypeptide with lysophospholipase activity and was isolated from Aspergillus and sequences derived therefrom, polypeptides with lysophospholipase activity encoded by these sequences as well as the use of these polypeptides for improving the filterability of syrups consisting of wheat starch and for related applications.Type: ApplicationFiled: May 10, 2012Publication date: August 30, 2012Applicant: AB ENZYMES GMBHInventors: Khanh Q. Nguyen, Volker Marschner, Kornelia Titze, Bruno Winter
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Patent number: 8202715Abstract: The invention relates to a DNA sequence that encodes a polypeptide with lysophospholipase activity and was isolated from Aspergillus and sequences derived therefrom, polypeptides with lysophospholipase activity encoded by these sequences as well as the use of these polypeptides for improving the filterability of syrups consisting of wheat starch and for related applications.Type: GrantFiled: September 21, 2007Date of Patent: June 19, 2012Assignee: AB Enzymes GmbHInventors: Khanh Q. Nguyen, Volker Marschner, Kornelia Titze, Bruno Winter
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Publication number: 20110287141Abstract: The invention relates to a DNA sequence that encodes a polypeptide with phospholipase activity and was isolated from Aspergillus and sequences derived therefrom, polypeptides with phospholipase activity encoded by these sequences as well as the use of these polypeptides for degumming of vegetable oil, for the preparation of dough and/or bakery products, for the preparation of dairy products, for processing steps in the textile industry and for related applications.Type: ApplicationFiled: June 28, 2011Publication date: November 24, 2011Applicant: AB ENZYMES GMBHInventors: Khanh Q. Nguyen, Volker Marschner, Kornelia Titze, Bruno Winter
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Publication number: 20110262619Abstract: The invention relates to a recombinant DNA molecule coding a polypeptide having phytase stability after being expressed in a prokaryotic or eukaryotic host cell. Said recombinant DNA molecule comprises a DNA sequence selected from among a) DNA sequences coding a polypeptide that has phytase activity and is obtained by varying the mature wild-type E. coli phytase sequence, the variation being selected from among i) the mutation lysine?aspartic acid in position 74 (K74D), and/or ii) a combination of the mutations asparagine?arginine in position 139 (N139R) and aspartic acid?glutamic acid in position 142 (D142E), and/or iii) a combination of the mutations leucine?isoleucine in position 145 (L145I) and leucine?isoleucine in position 198 (L198I), and/or iv) a mutation valine?proline in position 200 (V200P), and/or v) an N-terminal or C-terminal or an N-terminal and C-terminal addition of a sequence section of the acidic phosphatase of Aspergillus niger var.Type: ApplicationFiled: November 2, 2007Publication date: October 27, 2011Applicant: AB Enzymes GmbHInventors: Khanh Q Nguyen, Bruno Winter
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Patent number: 7993876Abstract: The invention relates to a DNA sequence that encodes a polypeptide with phospholipase activity and was isolated from Aspergillus and sequences derived therefrom, polypeptides with phospholipase activity encoded by these sequences as well as the use of these polypeptides for degumming of vegetable oil, for the preparation of dough and/or bakery products, for the preparation of dairy products, for processing steps in the textile industry and for related applications.Type: GrantFiled: September 21, 2007Date of Patent: August 9, 2011Assignee: AB Enzymes GmbHInventors: Khanh Q. Nguyen, Volker Marschner, Kornelia Titze, Bruno Winter
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Publication number: 20100248306Abstract: The invention relates to an expression system for the production of one or more target polypeptide/target polypeptides, comprising a host cell in whose genome the DNA sequence that codes glycerine-3-phosphate dehydrogenase is inactivated or partially or completely deleted and which is transformed by an extrachromosomal element that comprises a DNA sequence that codes the target polypeptide(s) and glycerine-3-phosphate dehydrogenase, whereby not only the host cell genome but also the extrachromosomal element do not carry an antibiotic-resistance gene, as well as a DNA sequence that codes for a polypeptide with glycerine-3-phosphate dehydrogenase activity characterized in that the DNA sequence is selected from a) DNA sequences that comprise a nucleotide sequence according to SEQ ID NO: 1, b) DNA sequences that comprise a nucleotide sequence represented by the nucleotides 1338 to 2375 of SEQ ID NO: 1, c) DNA sequences that are coded by the plasmid pTP01 with the plasmid map according to FIG.Type: ApplicationFiled: March 12, 2008Publication date: September 30, 2010Applicant: AB Enzymes GmbHInventors: Celine Cadot, Tina Ploss, Ruth Schwerdtfeger, Bruno Winter
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Publication number: 20100178671Abstract: The invention relates to a vector construct for expressing and secreting polypeptides in filamentous fungi, comprising, in 5?3? direction, in functional linkage, optionally a promoter which may have an enhancer placed upstream, at least one DNA sequence which codes for a first polypeptide and which may comprise a signal sequence or not, optionally a DNA sequence which codes for a 2A cleavage site or for a sequence derived therefrom, optionally together with an upstream DNA sequence which codes for a proteolytic cleavage site, or optionally a DNA sequence which codes for a proteolytic cleavage site, a DNA sequence which codes for a second polypeptide with signal sequence, optionally a further DNA sequence which codes for a 2A cleavage site or for a sequence derived therefrom, optionally together with an upstream DNA sequence which codes for a proteolytic cleavage site, or optionally a DNA sequence which codes for a proteolytic cleavage site, optionally a DNA sequence which codes for a third polypeptide withType: ApplicationFiled: July 19, 2007Publication date: July 15, 2010Applicant: AB ENZYMES GMBHInventors: Khanh Q. Nguyen, Bruno Winter
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Publication number: 20100119656Abstract: The invention relates to a DNA sequence that encodes a polypeptide with lysophospholipase activity and was isolated from Aspergillus and sequences derived therefrom, polypeptides with lysophospholipase activity encoded by these sequences as well as the use of these polypeptides for improving the filterability of syrups consisting of wheat starch and for related applications.Type: ApplicationFiled: September 21, 2007Publication date: May 13, 2010Applicant: AB Enzymes GmbHInventors: Khanh Q. Nguyen, Volker Marschner, Kornelia Titze, Bruno Winter