Abstract: Gram-negative bacterial cells for expression and secretion of heterologous peptides are disclosed. The bacterial cells include a first nucleic acid encoding a secretion signal sequence, e.g., a microcin secretion signal sequence, fused to a heterologous peptide, a second nucleic acid encoding a C39 peptidase-containing ATP-binding cassette transporter (PCAT), and a third nucleic acid encoding a membrane fusion protein. The gram-negative bacterial cells secrete the heterologous peptide from the bacterial cytosol to an external environment outside of the bacterial outer membrane. Methods for preparation and delivery of peptides to external environments are also described.

Abstract: The present disclosure provides ammonium compounds, e.g., compounds according to Formula I as set forth herein, which are useful as antimicrobial agents. Methods for the treatment of bacterial infections and associated conditions, e.g., gastrointestinal conditions, are also described, as well as methods for altering the microbiome of subjects such as humans.

Abstract: The present disclosure provides ammonium compounds, e.g., compounds according to Formula I as set forth herein, which are useful as antimicrobial agents. Methods for the treatment of bacterial infections and associated conditions, e.g., gastrointestinal conditions, are also described, as well as methods for altering the microbiome of subjects such as humans.

Abstract: Compositions and methods for isolating biologically active polypeptides (e.g., bacterial pro-growth polypeptides) are provided. In some aspects, bacterial cell populations are provided that express a surface-displayed library of candidate polypeptide sequences under the control of an inducible promoter.

Abstract: In an electronic device, plural circuit boards are plugged into respective connectors, or sockets, in a common backplane circuit board. The backplane maintains the flat circuit boards in fixed relation to one another. Each circuit board is provided with a respective optical transmitter and/or receiver to allow for the transmission of (typically digital) information via a high speed carrier in a light beam through unobstructed free space between the circuit boards. The circuit boards may also be provided with optical splitters and/or combiners as well as apertures to permit light signals to pass through the board's substrate to allow for communication between plural circuit boards. The circuit boards may further include small lenses and/or opaque elements to provide an optical path having selected physical characteristics.

Abstract: The invention relates to a process for the preparation of certain quinoline derivatives, in particular, 8-[(4-amino-1-methylbutyl)amino]-2,6-dimethoxy-4-methyl-5-(3-trifluoromethylphenoxy)quinoline succinate.