Abstract: Provided is a composition comprising a total dry weight of proteins and amino acids in of at least 80%; and wherein at least 95 wt % of said proteins have a mass of from about 70 to about 5,000 Daltons. Further provided is a growth medium comprising the composition, methods for the manufacture of the composition and uses thereof.

Abstract: Producing a composition comprising cells of at least one bacterium, where at least 90% of the composition is in the form of a free flowing powder, by providing a drum dryer comprising two spaced-apart rotatable cylinders, with a gap of width G inches and a length of L inches therebetween; providing a fermentation broth comprising cells of at least one bacterium in a liquid suspension at a total solid content C percent weight of no greater than about 15% wt of the total weight of the fermentation broth; and introducing the fermentation broth into said drum dryer at a rate of W Kg per hour of no greater than 14×[(G×L)/C] to provide the composition.

Abstract: Provided is a method for producing an ester of butyric acid from an acid mixture, comprising contacting an aqueous carboxylic acid mixture comprising butyric acid and at least one additional carboxylic acid selected from the group consisting of formic acid, acetic acid, propionic acid, lactic acid, hydroxybutyric acid and combinations thereof, wherein at least 30% of the total amount of acids in the aqueous carboxylic acid mixture are in free acid form, with a liquid extractant having a Hildebrand solubility parameter of less than 25 MPa(1/2); separating a resultant butyric acid-comprising extract from a residual aqueous phase; contacting a resultant separated butyric acid-comprising extract with an alkanol in the presence of an acid catalyst, to form a product comprising an ester of butyric acid and alkanol and optionally water; wherein when water is formed, separating water to provide a separated product comprising an ester of butyric acid.

Abstract: Provided are dry-milling methods for producing an aqueous fermentation feedstock ingredient. The methods include providing a corn-processing filtration feed; filtering at least a fraction of said filtration feed on a microfiltration membrane, whereby an aqueous filtration permeate and a filtration retentate are formed; separating said filtration permeate from said filtration retentate, to form separated permeate and separated retentate; forming a fermentation feedstock comprising said separated permeate, and saccharifying and/or washing said separated retentate.

Abstract: Provided is food component comprising cells of at least one bacterium, said cells comprising a crude protein concentration of at least 60 wt % of a total dry weight of said cells and nucleic acid at a concentration of less than about 5 wt % of a total dry weight of said cells, wherein at least 50% of a total number of said cells are dead cells. Further provided are food and beverages comprising such food components and methods of manufacture thereof.

Abstract: Provided is an isolated asporogenous strain of Clostridium tyrobutyricum bacterium comprising a single base pair mutation in a gene involved in the control of a sporulation process and methods of production thereof. Also provided is a feed ingredient comprising an isolated asporogenous strain of Clostridium tyrobutyricum bacterium, such as an asporogenous strain comprising a mutation in a gene involved in the control of a sporulation process.

Abstract: Provided is a method for the manufacture of a butyrate product by fermentation of a carbon source-containing fermentation feedstock with a Clostridia class bacterium which natively produces butyric acid while maintaining a pH of about 5 to 7, wherein ammonia is used for said maintaining pH and whereby a fermentation broth comprising ammonium butyrate, a biomass, and optionally a fermentation by-product is formed; separating said biomass from said fermentation broth to form separated biomass and a clarified fermentation broth, wherein said clarified fermentation broth comprises said ammonium butyrate and optionally said fermentation by-product; and adding to said clarified broth an additive selected from the group consisting of a mineral acid, a mineral base, a soluble calcium salt and combinations thereof, whereby said butyrate product is formed in said clarified broth. Further provided are feed ingredients, animal feeds and anti-icing agents comprising the composition, and uses thereof.

Abstract: Provided are aqueous fermentation feedstocks comprising glucose monomers at a concentration of less than 50 gram/Liter (g/L) of the total feedstock, water-soluble dextrose oligomers at a concentration in a range between 50 g/L and 300 g/L of the total feedstock; and water. Further provided are methods of production thereof and uses thereof in the production of single cell protein and/or ethanol.

Abstract: Aqueous fermentation feedstock and method of producing same. The feedstock includes glucose and dextrose oligomers, wherein (i) glucose concentration is in a range between 10 gram/Liter (g/L) and 150 g/L; (ii) dextrose oligomers concentration is in a range between 50 g/L and 300 g/L; and optionally (iii) slurried particles of less than 0.5 micron; (iv) slurried particles of more than 0.5 micron, wherein a content of such suspended particles of more than 0.5 micron is less than 30 g/L; (v) ash at a concentration in a range between 20 g/L and 50 g/L; (vi) lactate at a concentration in a range between 0.5 g/L and 10 g/L; (vii) protein at a concentration in a range between 5 g/L and 50 g/L; (viii) corn oil at a concentration of less than 10 g/L; and/or (ix) glycerol at a concentration in a range between 1 g/L and 30 g/L.

Abstract: A method for the production of a rhamnolipid. The method includes culturing in a fermentation medium an organism capable of producing a rhamnolipid to form a fermentation broth comprising at least one rhamnolipid and having a pH>5; extracting at least one lipophilic impurity from said fermentation broth; acidulating said fermentation broth to form an aqueous medium of pH<5 comprising the at least one rhamnolipid; extracting the at least one rhamnolipid from said acidulated fermentation broth; and separating the at least one rhamnolipid to obtain a purified rhamnolipid.

Abstract: Provided are dry-milling methods for producing an aqueous fermentation feedstock ingredient. The methods include providing a corn-processing filtration feed; filtering at least a fraction of said filtration feed on a microfiltration membrane, whereby an aqueous filtration permeate and a filtration retentate are formed; separating said filtration permeate from said filtration retentate, to form separated permeate and separated retentate; forming a fermentation feedstock comprising said separated permeate, and saccharifying and/or washing said separated retentate.

Abstract: Provided is an improved method for selecting a probiotic microorganism composition, the method comprising providing a first consortium of microorganisms having Microorganism Composition A; providing a first host animal having a first microbiome having Microorganism Composition B; administering said first consortium to said first host animal, whereby said first microbiome is converted to a second microbiome having Microorganism Composition C; collecting a first sample of said second microbiome; and transferring at least a fraction of said first sample and/or said treated first sample onto and/or into a growth medium and incubating at a temperature between 15 degrees Celsius and 70 degrees Celsius to form first selected microorganism composition having Microorganism Composition D; wherein Microorganism Composition A of said first consortium comprises at least 50 different strains; wherein each of Compositions A and B; B and C and D and A differ from each other.

Abstract: An integrated wet-mill method for the production of ethanol and single cell protein. An integrated method for the production of ethanol and a single cell protein product is described including providing corn kernels, which kernels comprise germ, fiber, protein and starch and which method includes, among other things, steeping said corn kernels and separating said steeped kernels from a steep liquor.

Abstract: Methods for production of a bioproduct with a microorganism and selective extraction of bioproducts from a fermentation broth. The methods may include mixing a carbon source, a nitrogen source, and an extractant-depleted raffinate to form a fermentation medium, and fermenting the medium with a microorganism to form a fermentation broth having at least one bioproduct. The bioproduct may be extracted from the fermentation broth with an extractant comprising an olefin to form an extract and a raffinate, and the extract may be further separated from the raffinate. The bioproduct may then be separated from the extract, and the extractant may be separated from the raffinate to regenerate the extract-depleted raffinate.

Abstract: Mixotrophic fermentation method performed to make one or more bioproducts such as alcohols, organic acids of less than 7 carbons, acetone, 2,3-butanediol and mixtures thereof with a microorganism. The mixotrophic fermentation method includes providing an isolated organism and providing a first feedstock and a second feedstock for use in a fermentation medium. The first feedstock includes a carbon source that is metabolized by the native form of the organism at a rate of less than 0.01 g/hr/g cell mass, and the second feedstock includes CO, CO2, carbonate, bicarbonate, H2, glycerol, methanol, formate, urea or mixtures thereof. The mixotrophic fermentation method also includes culturing the organism in the fermentation medium, whereby both feedstocks are metabolized and a fermentation broth is formed, the broth having at least one bioproduct. Optionally, the bioproduct may be separated from the broth.

Abstract: Provided are methods, systems and apparatuses to the energy efficient and selective extraction of biomolecules, e.g., small organic compounds, e.g., one or more C3-C9 alcohols, one or more C3-C5 carboxylic acids, and mixtures thereof, from an aqueous solution, particularly aqueous solutions containing the alcohol in dilute or low concentrations, for example, fermentation broths.

Abstract: The present invention relates to methods and compositions for engineering sporulating bacterial cells, particularly a cell of the class Clostridia. In particular, the present invention relates to the generation of sporulation deficient bacteria for the generation of industrial superior phenotypes.

Abstract: The present invention relates to methods and compositions for engineering Clostridia species. In particular, embodiments of the present invention relate to the expression of recombinant resolvase proteins in Clostridia species.

Abstract: The present invention relates to methods and compositions for engineering Clostridia species. In particular, embodiments of the present invention relate to the expression of recombinant resolvase proteins in Clostridia species.

Abstract: Expression of the SpoIIE gene in a solventogenic Clostridium cell is silenced and sporulation is abolished. The cell exhibits increased production of a solvent, such as butanol, relative to a wild-type solventogenic Clostridium cell and can be used for industrial-scale production of a chemical product. A method includes silencing the SpoIIE gene of the Clostridium cell via a homologous recombination method in which a resolvase gene is expressed. Another method includes increasing solvent production in bacterial cells by inoculating the cells with an inoculum of exponentially growing cells, wherein expression of a sporulation gene in the bacterial cells is inhibited or silenced and the cells of the inoculum are in a post-exponential phase of growth prior to inoculation.