Abstract: Described herein are kits, compositions, and methods for detecting porcine antigens in biological samples. The kit comprises a support, anti-porcine antibodies coupled to the support, and instructions for preparing lysates from a porcine donor source and contacting the lysates with the anti-porcine antibodies. The kit may be for use in determining the risk of transplant rejection in a human subject receiving or being considered for receiving a transplant from the porcine donor.

Abstract: The present invention provides unique and advantageous methods for selecting the negative control value to be used as a correction to the results obtained when measuring the reaction of a complex biological mixture in a multi-analyte assay. The methods of the subject invention are particularly advantageous because the negative control value(s) is/are generated using the same sample that is also being analyzed for the presence of the analytes of interest.

Abstract: A Raman spectroscopy technique allows an analyte, a paramagnetic particle, and a spectral enhancement particle to combine in solution and for the combination product to be localized by a magnetic field for analysis. The spectral enhancement particle may be comprised of an active SERS metal particle with or without a material coating. The spectral enhancement particle may function as a reporter for the presence of the analyte or merely increase the magnitude of the Raman spectrum of the analyte. The technique is applicable to both immunoassays and chemical assays. Multiple spectral enhancement particle reporters may be measured in a single assay that can detect multiple analytes using the SERS effect.

Abstract: The present invention provides unique and advantageous methods for selecting the negative control value to be used as a correction to the results obtained when measuring the reaction of a complex biological mixture in a multi-analyte assay. The methods of the subject invention are particularly advantageous because the negative control value(s) is/are generated using the same sample that is also being analyzed for the presence of the analytes of interest.