Patents by Inventor Bryan W. Kluttz
Bryan W. Kluttz has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 9562260Abstract: An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature.Type: GrantFiled: September 24, 2010Date of Patent: February 7, 2017Assignee: BIOMERIEUX, INC.Inventors: Bryan W. Kluttz, Geoff A. McKinley, Fabio Gennari, Michel Guy, Christopher Cotter, Luigi Catazariti, Louis Graziano, Bruno Colin, Cecile Paris, Jacque Dachaud
-
Publication number: 20110020921Abstract: An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature.Type: ApplicationFiled: September 24, 2010Publication date: January 27, 2011Applicant: bioMerieux, Inc.Inventors: Bryan W. Kluttz, Geoff A. McKinley, Fabio Gennari, Michel Guy, Christopher Cotter, Luigi Catanzariti, Louis Graziano, Bruno Colin, Cecile Paris, Jacques Dachaud
-
Patent number: 7807449Abstract: An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature.Type: GrantFiled: May 22, 2006Date of Patent: October 5, 2010Assignee: bioMerieux, Inc.Inventors: Bryan W. Kluttz, Geoff A. McKinley, Fabio Gennari, Michel Guy, Christopher Cotter, Luigi Catazariti, Louis Graziano, Bruno Colin, Cecile Paris, Jacque Dachaud
-
Publication number: 20080240985Abstract: The present invention relates to the detection of specific nucleic acid sequences, either by a process of amplification of specific nucleic acid sequences or not. More particularly the invention provides for improved compositions and methods for reducing the chance for contamination from manipulation of reagents, internal controls for amplification, and the use of automated apparatus for the automated detection of one, or more than one amplified nucleic acid sequences.Type: ApplicationFiled: July 19, 2007Publication date: October 2, 2008Inventors: Luigi Catanzariti, Bryan W. Kluttz, Marcela Vera-Garcia, J. Lawrence Burg, James G. Moe, Geoff A. McKinley
-
Patent number: 7309588Abstract: The present invention relates to the detection of specific nucleic acid sequences after an amplification process, or directly without amplification. In particular, the invention provides for the automation of the amplification and detection process, the amplification and detection of one or more specific nucleic acid sequences, the use of internal controls, reduced potential for contamination caused by the manual manipulation of reagents, and improved reagent compositions to better control assay performance and provide for further protection against contamination.Type: GrantFiled: July 9, 2001Date of Patent: December 18, 2007Assignee: bioMerieux, Inc.Inventors: J. Lawrence Burg, Bryan W. Kluttz, Luigi Catanzariti, Marcela Vera-Garcia, James G. Moe, Geoff A. McKinley
-
Patent number: 7262008Abstract: The present invention relates to the detection of specific nucleic acid sequences, either by a process of amplification of specific nucleic acid sequences or not. More particularly the invention provides for improved compositions and methods for reducing the chance for contamination from manipulation of reagents, internal controls for amplification, and the use of automated apparatus for the automated detection of one, or more than one amplified nucleic acid sequences.Type: GrantFiled: August 8, 2002Date of Patent: August 28, 2007Assignee: bioMerieux, Inc.Inventors: Luigi Catanzariti, Bryan W. Kluttz, Marcela Vera-Garcia, J. Lawrence Burg, James G. Moe, Geoff A. McKinley
-
Patent number: 7214529Abstract: An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature.Type: GrantFiled: July 12, 2005Date of Patent: May 8, 2007Assignee: bioMérieux, Inc.Inventors: Bryan W. Kluttz, Geoff A. McKinley, Fabio Gennari, Michel Guy, Christopher Cotter, Luigi Catanzariti, Louis Graziano, Bruno Colin, Cecile Jaravel, Jacques Dachaud
-
Patent number: 6949376Abstract: An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature.Type: GrantFiled: May 7, 2002Date of Patent: September 27, 2005Assignee: bioMerieux, Inc.Inventors: Bryan W. Kluttz, Geoff A. McKinley, Fabio Gennari, Michel Guy, Christopher Cotter, Luigi Catanzariti, Louis Graziano, Bruno Colin, Cecile Jaravel, Jacques Dachaud
-
Publication number: 20040248087Abstract: The present invention relates to the detection of specific nucleic acid sequences after an amplification process, or directly without amplification. In particular, the invention provides for the automation of the amplification and detection process, the amplification and detection of one or more specific nucleic acid sequences, the use of internal controls, reduced potential for contamination caused by the manual manipulation of reagents, and improved reagent compositions to better control assay performance and provide for further protection against contamination.Type: ApplicationFiled: July 9, 2001Publication date: December 9, 2004Inventors: J. Lawrence Burg, Bryan W. Kluttz, Luigi Catanzariti, Marcela Vera-Garcia, James G. Moe, Geoff A. McKinley
-
Patent number: 6558901Abstract: The present invention relates to the detection of specific nucleic acid sequences, either by a process of amplification of specific nucleic acid sequences or not. More particularly the invention provides for improved compositions and methods for reducing the chance for contamination from manipulation of reagents, internal controls for amplification, and the use of automated apparatus for the automated detection of one, or more than one amplified nucleic acid sequences.Type: GrantFiled: April 24, 1998Date of Patent: May 6, 2003Assignee: bioMerieux VitekInventors: Luigi Catanzariti, Bryan W. Kluttz, Marcela Vera-Garcia, J. Lawrence Burg, James G. Moe, Geoff A. McKinley
-
Publication number: 20030077580Abstract: The present invention relates to the detection of specific nucleic acid sequences, either by a process of amplification of specific nucleic acid sequences or not. More particularly the invention provides for improved compositions and methods for reducing the chance for contamination from manipulation of reagents, internal controls for amplification, and the use of automated apparatus for the automated detection of one, or more than one amplified nucleic acid sequences.Type: ApplicationFiled: August 8, 2002Publication date: April 24, 2003Applicant: bioMerieux VitekInventors: Luigi Catanzariti, Bryan W. Kluttz, Marcela Vera-Garcia, J. Lawrence Burg, James G. Moe, Geoff A. McKinley
-
Patent number: 6528632Abstract: The present invention relates to the detection of specific nucleic acid sequences after an amplification process, or directly without amplification. In particular, the invention provides for the automation of the amplification and detection process, the amplification and detection of one or more specific nucleic acid sequences, the use of internal controls, reduced potential for contamination caused by the manual manipulation of reagents, and improved reagent compositions to better control assay performance and provide for further protection against contamination.Type: GrantFiled: May 31, 2000Date of Patent: March 4, 2003Assignee: bioMerieux VitekInventors: Luigi Catanzariti, Bryan W. Kluttz, Marcela Vera-Garcia, J. Lawrence Burg, James G. Moe, Geoff A. McKinley
-
Publication number: 20020127708Abstract: An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature.Type: ApplicationFiled: May 7, 2002Publication date: September 12, 2002Inventors: Bryan W. Kluttz, Geoff A. McKinley, Fabio Gennari, Michel Guy, Christopher Cotter, Luigi Catanzariti, Louis Graziano, Bruno Colin, Cecile Paris, Jacques Dachaud
-
Patent number: 6429007Abstract: An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature.Type: GrantFiled: October 18, 1999Date of Patent: August 6, 2002Assignee: bioMérieux, Inc.Inventors: Bryan W. Kluttz, Geoff A. McKinley, Fabio Gennari, Michel Guy, Christopher Cotter, Luigi Catanzariti, Louis Graziano, Bruno Colin, Cecile Jaravel, Jacques Dachaud
-
Patent number: 6300068Abstract: The present invention relates to the detection of specific nucleic acid sequences after an amplification process, or directly without amplification. In particular, the invention provides for the automation of the amplification and detection process, the amplification and detection of one or more specific nucleic acid sequences, the use of internal controls, reduced potential for contamination caused by the manual manipulation of reagents, and improved reagent compositions to better control assay performance and provide for further protection against contamination.Type: GrantFiled: February 5, 1999Date of Patent: October 9, 2001Assignee: bioMerieux Vitek, Inc.Inventors: J. Lawrence Burg, Luigi Catanzariti, Bryan W. Kluttz, Geoff A. McKinley, James G. Moe, Marcela Vera-Garcia
-
Patent number: 6103503Abstract: A testing apparatus is described that contains a test sample and one or more reagents, or reaction solutions associated with chemical reactions and a resilient, compressible, porous material, and which is amenable to subsequent process or reaction steps or liquid transfer steps. A piece of sponge or foam rubber, which is compatible with the amplification reactions and amplification products, is introduced into the reaction vessel and absorbs the test sample and reagents, or reaction solutions associated with an amplification process, or amplification products thus, reducing their loss through aerosolation.Type: GrantFiled: June 30, 1998Date of Patent: August 15, 2000Assignee: bio Merieux, Inc.Inventors: Bryan W. Kluttz, Arthur L. Garland, Geoff A. McKinley, Luigi Catanzariti
-
Patent number: 5989499Abstract: A reaction vessel for a nucleic acid amplification reaction has a first chamber containing an amplification reagent mix, a second chamber containing an amplification enzyme, and a fluid channel or chamber connecting the first and second chambers together. A fluid sample is introduced into the first chamber. After a denaturation and primer annealing process has occurred in the first chamber, the fluid channel is opened to allow the solution of the reagent and fluid sample to flow into the second chamber. The second chamber is maintained at an optimal temperature for the amplification reaction.A station is described for processing test strips incorporating the reaction vessels. The station includes temperature and vacuum control subsystems to maintain proper temperatures in the reaction vessel and effectuate the transfer of the fluid from one chamber to the other in an autlomated fashion without human intervention.Type: GrantFiled: April 2, 1998Date of Patent: November 23, 1999Assignee: bioMerieux, Inc.Inventors: Luigi Catanzariti, Bryan W. Kluttz, Bruno Colin, Cecile Jaravel
-
Patent number: 5786182Abstract: A reaction vessel for a nucleic acid amplification reaction has a first chamber containing an amplification reagent mix, a second chamber containing an amplification enzyme, and a fluid channel or chamber connecting the first and second chambers together. A fluid sample is introduced into the first chamber. After a denaturation and primer annealing process has occurred in the first chamber, the fluid channel is opened to allow the solution of the reagent and fluid sample to flow into the second chamber. The second chamber is maintained at an optimal temperature for the amplification reaction.A station is described for processing test strips incorporating the reaction vessels. The station includes temperature and vacuum control subsystems to maintain proper temperatures in the reaction vessel and effectuate the transfer of the fluid from one chamber to the other in an automated fashion without human intervention.Type: GrantFiled: May 2, 1997Date of Patent: July 28, 1998Assignee: bioMerieux Vitek, Inc.Inventors: Luigi Catanzariti, Bryan W. Kluttz, Geoff A. McKinley, Arthur L. Garland, Louis Graziano, James G. Moe, Marcela Vera-Garcia, James Clement Bishop, David Chastain, Fabio Gennari