Abstract: Provided herein are, inter alia, compositions and methods for improved production of SC-? cells in vitro. For example, provided are novel formulations and differentiation methods that result in higher cell yields and recoveries, increased numbers and relative percentages of SC-? cells, enhanced stability and shelf-life of SC-? cells, SC-islet clusters with advantageous characteristics such as reduced size and increased uniformity, improved function of the SC-? cells in vitro, and improved viability, function, and reduced immunogenicity after transplantation. The disclosed compositions and methods can be employed in the manufacture of SC-islets for human therapeutic use.

Abstract: Provided herein are methods of manufacturing ? cells in vitro. Also provided herein are methods of treating a disease in a subject comprising administering the ? cells manufactured in vitro to the subject. Also provided herein are methods of differentiating stem cells into ? cells.