Abstract: A multiwell plate device is provided having a frame, a substantially flat substrate and a multiwell structure supported by the substrate. The multiwell structure includes multiple bottomless wells formed therein. The substrate is supported by the frame and may be processed by an automated arrayer or instrument that is used to print or spot arrays in a pattern on a reaction surface of the substrate. Thereafter, the multiwell structure may be engaged with the substrate and the multiwell structure and substrate may be engaged with the frame in an upright orientation. For scanning or other analysis, the multiwell structure and substrate may be disengaged from the frame, inverted 180°, and then reengaged with the frame in the inverted orientation.

Abstract: The present invention relates to novel methodologies for performing multiplexed assays for biological molecules such as proteins and nucleic acids. In particular, the present invention provides multiplexed assays using precipitating reagents and optically clear nitrocellulose-coated solid supports.

Abstract: The present invention relates to novel methodologies for performing multiplexed assays with high precision and sensitivity. In particular, the present invention relates to improving assay sensitivity and precision by combining the normalization of multiplexed assay data using an internal standard with scattered application of samples and standards replicates throughout sample wells on a slide or set of slides as well as scattered replicates of arrayed probes in a single well. These compositions and methods can be used to perform multiplexed assays for analytes in patient and other test samples. In particular, these methods have applications for Quantitative Multi-analyte Immunoassays (QMI) to measure proteins in human serum and plasma.

Abstract: A multiwell plate device is provided having a frame, a substantially flat substrate and a multiwell structure supported by the substrate. The multiwell structure includes multiple bottomless wells formed therein. The substrate is supported by the frame and may be processed by an automated arrayer or instrument that is used to print or spot arrays in a pattern on a reaction surface of the substrate. Thereafter, the multiwell structure may be engaged with the substrate and the multiwell structure and substrate may be engaged with the frame in an upright orientation. For scanning or other analysis, the multiwell structure and substrate may be disengaged from the frame, inverted 180°, and then reengaged with the frame in the inverted orientation.

Abstract: The present invention relates to novel methods for the analysis of molecular interactions. In particular, the present invention relates to compositions and methods for molecular interaction assays performed in solution and on solid surfaces. The present invention thus provides improves methods and compositions for the analysis of molecular interactions.

Abstract: Disclosed is a method to detect unlabeled nucleic acids (DNA and/or RNA) in a taxa, species, and organelle-specific fashion using surface plasmon resonance (SPR) imaging. Taxa-specific, species-specific, or organelle-specific nucleic acids are affixed to an SPR-suitable substrate. A nucleic acid sample to be analyzed is then contacted with the SPR-substrate and the substrate analyzed to determine the presence or absence of specific hybridization between the nucleic acids bound to the substrate and the nucleic acids contained in the sample. The method does not require that either the bound nucleic acids nor the sample nucleic acids be labeled. The method can be used to identify the source of nucleic acids, their sequence, as well as to identify organisms and place them within a given taxonomic hierarchy.

Abstract: The present invention relates to novel methods for the analysis of interactions of target molecules and cells. In particular, the present invention relates to compositions and methods for the detection of molecules involved in various aspects of cellular development. The present invention further provides methods of screening molecules for association with cellular development.

Abstract: The present invention relates to novel methods for the analysis of interactions of transcription factors with target nucleic acids. In particular, the present invention relates to compositions and methods for the detection of transcription factors binding to their target promoter regions. The present invention further provides methods of screening compounds for their ability to alter such binding interactions.

Abstract: The present invention relates to novel components for surface plasmon resonance (SPR) detection of molecular interactions. In particular, the present invention relates to disposable arrayed prisms for use in SPR. The present invention provides improved prisms comprising target biological macromolecules for use in SPR.

Abstract: The present invention relates to novel methods for the analysis of molecular interactions. In particular, the present invention relates to compositions and methods for molecular interaction assays performed in solution and on solid surfaces. The present invention thus provides improves methods and compositions for the analysis of molecular interactions.

Abstract: The present invention relates to novel methods for the analysis of interactions of transcription factors with target nucleic acids. In particular, the present invention relates to compositions and methods for the detection of transcription factors binding to their target promoter regions. The present invention further provides methods of screening compounds for their ability to alter such binding interactions.

Abstract: Disclosed is a method to detect unlabeled nucleic acids (DNA and/or RNA) in a taxa, species, and organelle-specific fashion using surface plasmon resonance (SPR) imaging. Taxa-specific, species-specific, or organelle-specific nucleic acids are affixed to an SPR-suitable substrate. A nucleic acid sample to be analyzed is then contacted with the SPR-substrate and the substrate analyzed to determine the presence or absence of specific hybridization between the nucleic acids bound to the substrate and the nucleic acids contained in the sample. The method does not require that either the bound nucleic acids nor the sample nucleic acids be labeled. The method can be used to identify the source of nucleic acids, their sequence, as well as to identify organisms and place them within a given taxonomic hierarchy.