Patents by Inventor Bunsei Kawakami
Bunsei Kawakami has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8552170Abstract: The present invention provides an expression vector which is effective in an efficient establishment of transformed cells which express the aimed protein gene in a high level. An expression vector which has a cassette for expressing the drug selective marker gene containing mRNA destabilizing sequence, at least one element for stabilizing the gene expression and a cassette for expressing the gene of the aimed protein. Preferably, the mRNA destabilizing sequence is derived from AT-rich sequence existing in the 3?-untranslated region of cytokine, interleukin or proto-oncogene, and the element for stabilizing the gene expression is derived from Chinese hamster genome.Type: GrantFiled: June 4, 2010Date of Patent: October 8, 2013Assignee: Toyo Boseki Kabushiki KaishaInventors: Tomomi Yamazaki, Kenji Masuda, Shigeaki Nishii, Bunsei Kawakami
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Publication number: 20110281286Abstract: The present invention provides an expression vector which is effective in an efficient establishment of transformed cells which express the aimed protein gene in a high level. An expression vector which has a cassette for expressing the drug selective marker gene containing mRNA destabilizing sequence, at least one element for stabilizing the gene expression and a cassette for expressing the gene of the aimed protein. Preferably, the mRNA destabilizing sequence is derived from AT-rich sequence existing in the 3?-untranslated region of cytokine, interleukin or proto-oncogene, and the element for stabilizing the gene expression is derived from Chinese hamster genome.Type: ApplicationFiled: June 4, 2010Publication date: November 17, 2011Applicant: TOYO BOSEKI KABUSHIKI KAISHAInventors: Tomomi Yamazaki, Kenji Masuda, Shigeaki Nishii, Bunsei Kawakami
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Patent number: 7507567Abstract: The present application relates to mutated RNA polymerases from bacteriophages that have increased stability, for example under high temperature conditions. Preferred mutated RNA polymerases according to the invention are mutant RNA polymerases from T7 or SP3 bacteriophages. An especially preferred embodiment of the present invention is a T7 RNA polymerase with a serine to proline amino acid change in the protein at position 633 of the amino acid sequence.Type: GrantFiled: March 1, 2001Date of Patent: March 24, 2009Assignee: bioMerieus B.V.Inventors: Akio Sugiyama, Yoshiaki Nishiya, Bunsei Kawakami
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Patent number: 7422882Abstract: An object of the present invention is to provide a thermostable DNA polymerase with enhanced amplification efficiency and/or improved fidelity in polymerase chain reaction (PCR), and provide a process for production thereof. More specifically, the present invention provides thermostable DNA polymerase wherein in the DX1EX2X3X4H sequence (D: aspartic acid, E: glutamic acid, H: histidine, X1, X2, X3 and X4: any amino acid) consisting of DX1E sequence within the EXO I region and a four amino acid length peptide adjacent to said glutamic acid(E) of thermostable DNA polymerase having 3?-5? exonuclease activity, histidine(H) has been replaced by another amino acid.Type: GrantFiled: May 10, 2001Date of Patent: September 9, 2008Assignee: Toyo Boseki Kabushiki KaishaInventors: Toshihiro Kuroita, Masao Kitabayashi, Yoshikazu Ishida, Hideyuki Komatsubara, Yoshiaki Nishiya, Bunsei Kawakami, Yoshihisa Kawamura, Tadayuki Imanaka
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Patent number: 7048915Abstract: The present invention provides a composition for cell-free protein synthesis, which is superior in storage stability in a freeze-dried state, more particularly a freeze-dryable or freeze-dried composition for cell-free protein synthesis, which contains a cell extract for cell-free protein synthesis and inositol, and a freeze-dryable or freeze-dried composition for cell-free protein synthesis containing a cell extract for cell-free protein synthesis, and a deliquescent material in a proportion of not more than 0.01 part by weight per part by weight of a protein in the composition; and a composition for cell-free protein synthesis superior in storage stability in a frozen state, more particularly a freezable or frozen composition for cell-free protein synthesis, containing a cell extract for cell-free protein synthesis and polyhydric alcohol.Type: GrantFiled: April 18, 2002Date of Patent: May 23, 2006Assignee: CellFree Sciences Co., Ltd.Inventors: Toshihiro Kuroita, Bunsei Kawakami, Yoshihisa Kawamura, Shigemichi Nishikawa, Yaeta Endo
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Publication number: 20040191815Abstract: The array of the present invention has a plurality of double-stranded oligonucleotides immobilized on a metal substrate. Each of the double-stranded oligonucleotides includes a first single-stranded oligonucleotide and a second single-stranded oligonucleotide. The first and second single-stranded oligonucleotides are entirely or partially bonded together in a complementary manner to form said double-stranded oligonucleotide. Among the first and second single-stranded oligonucleotides, only the first single-stranded oligonucleotide is bonded on said substrate. The present invention also relates to a method for analyzing the interaction between the immobilized biomolecules and another biomolecule or an aggregate thereof by use of the array.Type: ApplicationFiled: January 14, 2004Publication date: September 30, 2004Inventors: Motoki Kyo, Masayuki Yamamoto, Hozumi Motohashi, Kinuko Ohneda, Yutaka Takarada, Bunsei Kawakami, Yoshihisa Kawamura
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Publication number: 20030199076Abstract: The present invention provides a composition for cell-free protein synthesis, which is superior in storage stability in a freeze-dried state, more particularly a freeze-dryable or freeze-dried composition for cell-free protein synthesis, which contains a cell extract for cell-free protein synthesis and inositol, and a freeze-dryable or freeze-dried composition for cell-free protein synthesis containing a cell extract for cell-free protein synthesis, and a deliquescent material in a proportion of not more than 0.01 part by weight per part by weight of a protein in the composition; and a composition for cell-free protein synthesis superior in storage stability in a frozen state, more particularly a freezable or frozen composition for cell-free protein synthesis, containing a cell extract for cell-free protein synthesis and polyhydric alcohol.Type: ApplicationFiled: April 18, 2002Publication date: October 23, 2003Applicants: Toyo Boseki Kabushiki Kaisha, WAKENYAKU CO., LTD., Yaeta EndoInventors: Toshihiro Kuroita, Bunsei Kawakami, Yoshihisa Kawamura, Shigemichi Nishikawa, Yaeta Endo
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Publication number: 20030175738Abstract: The present application relates to mutated RNA polymerases from bacteriophages that have increased stability, for example under high temperature conditions. Preferred mutated RNA polymerases according to the invention are mutant RNA polymerases from T7 or SP3 bacteriophages. An especially preferred embodiment of the present invention is a T7 RNA polymerase with a serine to proline amino acid change in the protein at position 633 of the amino acid sequence.Type: ApplicationFiled: February 11, 2003Publication date: September 18, 2003Inventors: Akio Sugiyama, Yoshiaki Nishiya, Bunsei Kawakami
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Publication number: 20020076768Abstract: An object of the present invention is to provide a thermostable DNA polymerase with enhanced amplification efficiency and/or improved fidelity in polymerase chain reaction (PCR), and provide a process for production thereof. More specifically, the present invention provides thermostable DNA polymerase wherein in the DX1EX2X3X4H sequence (D: aspartic acid, E: glutamic acid, H: histidine, X1, X2, X3 and X4: any amino acid) consisting of DX1E sequence within the EXO I region and a four amino acid length peptide adjacent to said glutamic acid(E) of thermostable DNA polymerase having 3′-5′ exonuclease activity, histidine(H) has been replaced by another amino acid.Type: ApplicationFiled: May 10, 2001Publication date: June 20, 2002Applicant: Toyo Boseki Kabushiki KaishaInventors: Toshihiro Kuroita, Masao Kitabayashi, Yoshikazu Ishida, Hideyuki Komatsubara, Yoshiaki Nishiya, Bunsei Kawakami, Yoshihisa Kawamura, Tadayuki Imanaka
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Patent number: 6225065Abstract: A nucleic acid amplifying enzyme having a short reaction time and high fidelity is provided. The enzyme of this invention is a thermostable DNA polymerase having a nucleic acid extension rate of at least 30 bases per second and a 3′-5′ exonuclease activity. Also provided are a method and kit for amplifying nucleic acid.Type: GrantFiled: October 14, 1999Date of Patent: May 1, 2001Assignee: Toyo Boseki Kabushiki KaishaInventors: Masao Kitabayashi, Taku Arakawa, Hiroaki Inoue, Bunsei Kawakami, Yoshihisa Kawamura, Tadayuki Imanaka, Masahiro Takagi, Masaaki Morikawa
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Patent number: 6090256Abstract: An electrophoresis apparatus comprising an arched lid 2 covering an electrophoresis tank 1, a suction opening 4 set in one end of the lid 2 and an exhaust opening 7 set in the other end of the lid 2. On the outside of the suction opening 4 is set a fan 5. The fan 5 absorbs the outside air, which flows along the inner side of the lid 2 and is exhausted from the exhaust opening 7 together with the vapor generated during electrophoresis, thereby preventing clouding and bedewing of the inner surface of the lid. Consequently, the inside of the electrophoresis tank 1 can be safely observed without opening the transparent or semi-transparent lid 2 but through it.Type: GrantFiled: April 9, 1998Date of Patent: July 18, 2000Assignee: Toyo Boseki Kabushiki KaishaInventors: Yoichi Kadokami, Bunsei Kawakami, Yoshihisa Kawamura
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Patent number: 6054301Abstract: A nucleic acid amplifying enzyme having a short reaction time and high fidelity is provided. The enzyme of this invention is a thermostable DNA polymerase having a nucleic acid extension rate of at least 30 bases per second and a 3'-5' exonuclease activity. Also provided are a method and kit for amplifying nucleic acid.Type: GrantFiled: May 24, 1996Date of Patent: April 25, 2000Assignee: Toyo Boseki Kabushiki KaishaInventors: Masao Kitabayashi, Taku Arakawa, Hiroaki Inoue, Bunsei Kawakami, Yoshihisa Kawamura, Tadayuki Imanaka, Masahiro Takagi, Masaaki Morikawa
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Patent number: 6033859Abstract: A nucleic acid amplifying enzyme having a short reaction time and high fidelity is provided. The enzyme of this invention is a thermostable DNA polymerase having a nucleic acid extension rate of at least 30 bases per second and a 3'-5' exonuclease activity. Also provided are a method and kit for amplifying nucleic acid.Type: GrantFiled: May 6, 1998Date of Patent: March 7, 2000Assignee: Toyo Boseki Kabushiki KaishaInventors: Masao Kitabayashi, Taku Arakawa, Hiroaki Inoue, Bunsei Kawakami, Yoshihisa Kawamura, Tadayuki Imanaka, Masahiro Takagi, Masaaki Morikawa
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Patent number: 6008025Abstract: A modified thermostable DNA polymerase having 5% or less of the 3'-5' exonuclease activity of the enzyme before modification and a DNA polymerase composition for amplifying nucleic acid, which comprises the modified thermostable DNA polymerase having 0 to 5% of the 3'-5' exonuclease activity of the enzyme before modification and an unmodified thermostable DNA polymerase having 3'-5' exonuclease activity or a modified thermostable DNA polymerase having 100 to 6% of the 3'-5' exonuclease activity of a thermostable DNA polymerase before modification; a method for amplifying nucleic acid by use of said modified thermostable polymerase or said DNA polymerase composition; and a reagent therefor.Type: GrantFiled: July 29, 1997Date of Patent: December 28, 1999Assignee: Toyo Boseki Kabushiki KaishaInventors: Hideyuki Komatsubara, Masao Kitabayashi, Hideki Kamimura, Bunsei Kawakami, Yoshihisa Kawamura, Masahiro Takagi, Tadayuki Imanaka
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Patent number: 5990302Abstract: A method for isolating a ribonucleic acid, which comprises dissolution of a sample containing the ribonucleic acid, such as cells, in an acidic solution containing a lithium salt and a chaotropic agent, bringing the ribonucleic acid into contact with a nucleic acid-binding carrier such as silica particles, thereby to allow selective adsorption of the ribonucleic acid alone onto said carrier, and eluting the ribonucleic acid from the nucleic acid-bound carrier; a reagent therefor; and a method for producing a cDNA from the ribonucleic acid isolated by this method. According to the present invention, a high purity ribonucleic acid can be isolated quickly and safely from a sample containing the ribonucleic acid.Type: GrantFiled: July 11, 1997Date of Patent: November 23, 1999Assignee: Toyo Boseki Kabushiki KaishaInventors: Toshihiro Kuroita, Hideki Kamimura, Bunsei Kawakami, Yoshihisa Kawamura
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Patent number: 5049501Abstract: A recombinant vector comprising an incorporated chromosome DNA fragment containing a PvuI restriction enconuclease gene derived from Proteus vulgaris, a host transformed with the recombinant vector and a method of producing PvuI restriction endonuclease characterized in that the transformed host is cultivated and PvuI restriction endonuclease is harvested from the resulting culture. Since the host transformed with the recombinant vector of the present invention produces PvuI alone, it is unnecessary to remove PvuII in the purification process for PvuI, and further, since its productivity for nonspecific DNase is lower in comparison with conventional producer bacteria, DNase can be removed easily, making the production of PvuI easy.Type: GrantFiled: December 14, 1989Date of Patent: September 17, 1991Assignee: Toyo Boseki Kabushiki KaishaInventors: Nobuhiro Katsuragi, Bunsei Kawakami, Yoshihiko Maekawa