Patents by Inventor Burkhard Wilms

Burkhard Wilms has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11230718
    Abstract: The present invention is based on the finding that the combination of a specific 5?UTR polynucleotide sequence (see SEQ ID NO 1) and the hCD33 secretory leader sequence in an expression cassette for expressing a polypeptide of interest results in a surprisingly better expression level of the polypeptide of interest compared to prior art expression cassettes. Based on this finding, the present invention inter alia provides novel expression cassettes, expression vectors and methods for producing a polypeptide of interest with high yield.
    Type: Grant
    Filed: April 21, 2017
    Date of Patent: January 25, 2022
    Assignee: Novartis AG
    Inventors: Zorica Dragic, Sabine Geisse, Rita Schmitz, Burkhard Wilms
  • Patent number: 10724033
    Abstract: A method for selecting mammalian host cells that express a polypeptide of interest with high yield is described. The host cells comprise an expression cassette comprising at least a first polynucleotide encoding the polypeptide of interest, at least one leaky stop codon downstream of the first polynucleotide, and a second polynucleotide downstream of the leaky stop codon encoding an immunoglobulin transmembrane anchor. The host cells are cultivated to allow expression of the polypeptide of interest such that at least a portion of the polypeptide of interest is expressed as a fusion polypeptide that is displayed on the cell surface. The cells are selected based upon the presence or amount of the displayed fusion polypeptide. Also provided are methods for producing a polypeptide that employ a respective selection to identify high expressing host cells.
    Type: Grant
    Filed: August 8, 2017
    Date of Patent: July 28, 2020
    Assignee: Novartis AG
    Inventors: Thomas Jostock, Hans-Peter Knopf, Burkhard Wilms, Audrey Nommay
  • Publication number: 20170356006
    Abstract: The present invention is based on the finding that the combination of a specific 5?UTR polynucleotide sequence (see SEQ ID NO 1) and the hCD33 secretory leader sequence in an expression cassette for expressing a polypeptide of interest results in a surprisingly better expression level of the polypeptide of interest compared to prior art expression cassettes. Based on this finding, the present invention inter alia provides novel expression cassettes, expression vectors and methods for producing a polypeptide of interest with high yield.
    Type: Application
    Filed: April 21, 2017
    Publication date: December 14, 2017
    Applicant: NOVARTIS AG
    Inventors: Zorica DRAGIC, Sabine GEISSE, Rita SCHMITZ, Burkhard WILMS
  • Publication number: 20170342403
    Abstract: The present invention inter alia pertains to a method for generating or selecting a eukaryotic host cell expressing a desired level of a polypeptide of interest, comprising: a) providing a plurality of eukaryotic host cells comprising a heterologous nucleic acid comprising at least one cassette (Cas-POI) comprising at least a first polynucleotide (Pn-POI) encoding the polypeptide of interest, at least one stop codon downstream of the first polynucleotide, and a second polynucleotide downstream of the stop codon encoding an immunoglobulin transmembrane anchor or a functional variant thereof; b) cultivating the eukaryotic host cells to allow expression of the polypeptide of interest such that at least a portion of the polypeptide of interest is expressed as a fusion polypeptide comprising the immunoglobulin transmembrane anchor or a functional variant thereof, wherein said fusion polypeptide is being displayed on the surface of said host cell; c) selecting at least one eukaryotic host cell based upon the pre
    Type: Application
    Filed: August 8, 2017
    Publication date: November 30, 2017
    Applicant: NOVARTIS AG
    Inventors: Thomas Jostock, Hans-Peter Knopf, Burkhard Wilms, Audrey Nommay
  • Patent number: 9758779
    Abstract: The application describes a method of selecting mammalian host cells that express a polypeptide of interest with high yield. The host cells contain an expression cassette with a first polynucleotide encoding a polypeptide of interest, at least one leaky stop codon located downstream of the first polynucleotide and a second polynucleotide located downstream of the leaky codon encoding an immunoglobulin transmembrane anchor comprising a cytoplasmic domain. The host cells are cultivated to allow expression of the polypeptide of interest such that some of the polypeptides of interest are expressed as fusion proteins displayed on the cell surface. High producing cells are then selected based on the presence or amount of the displayed fusion polypeptides.
    Type: Grant
    Filed: August 28, 2009
    Date of Patent: September 12, 2017
    Assignee: Novartis AG
    Inventors: Thomas Jostock, Hans-Peter Knopf, Burkhard Wilms, Audrey Nommay
  • Patent number: 9663797
    Abstract: The present invention is based on the finding that the combination of a specific 5?UTR polynucleotide sequence (see SEQ ID NO 1) and the hCD33 secretory leader sequence in an expression cassette for expressing a polypeptide of interest results in a surprisingly better expression level of the polypeptide of interest compared to prior art expression cassettes. Based on this finding, the present invention inter alia provides novel expression cassettes, expression vectors and methods for producing a polypeptide of interest with high yield.
    Type: Grant
    Filed: November 18, 2013
    Date of Patent: May 30, 2017
    Assignee: NOVARTIS AG
    Inventors: Zorica Dragic, Sabine Geisse, Rita Schmitz, Burkhard Wilms
  • Patent number: 9422574
    Abstract: The invention provides vector nucleic acid for expressing at least one polypeptide of interest in a mammalian cell, comprising (a) at least one expression cassette (POI) for expressing a polypeptide of interest; (b) an expression cassette (MSM) comprising a mammalian selectable marker gene; (c) an expression cassette (MASM) comprising a mammalian amplifiable, selectable marker gene; wherein the expression cassette (POI) is flanked 5? by the expression cassette (MASM), the expression cassette (MSM) is located 3? from the expression cassette (POI) and wherein the expression cassettes (MASM), (POI) and (MSM) are arranged in the same 5? to 3? orientation. Also provided are host cells, comprising said vector and methods for producing a polypeptide using respective host cells.
    Type: Grant
    Filed: May 29, 2012
    Date of Patent: August 23, 2016
    Assignee: Novartis AG
    Inventors: Hans-Peter Knopf, Burkhard Wilms
  • Publication number: 20160186206
    Abstract: The present invention is based on the finding that the combination of a specific 5?UTR polynucleotide sequence (see SEQ ID NO 1) and the hCD33 secretory leader sequence in an expression cassette for expressing a polypeptide of interest results in a surprisingly better expression level of the polypeptide of interest compared to prior art expression cassettes. Based on this finding, the present invention inter alia provides novel expression cassettes, expression vectors and methods for producing a polypeptide of interest with high yield.
    Type: Application
    Filed: November 18, 2013
    Publication date: June 30, 2016
    Inventors: Zorica DRAGIC, Sabine GEISSE, Rita SCHMITZ, Burkhard WILMS
  • Publication number: 20160017319
    Abstract: A method of screening cell clones expressing a high yield of a polypeptide of interest is provided. The method employs the consecutive use of fluorescence activated cell sorting followed by colony picking based selection of cell clones with high expression rates and high proliferation rates. Furthermore, the invention pertains to a method of producing a polypeptide of interest using cells obtained by the described screening method.
    Type: Application
    Filed: March 10, 2014
    Publication date: January 21, 2016
    Inventors: Audrey NOMMAY, Burkhard WILMS
  • Publication number: 20120271040
    Abstract: The invention provides vector nucleic acid for expressing at least one polypeptide of interest in a mammalian cell, comprising (a) at least one expression cassette (POI) for expressing a polypeptide of interest; (b) an expression cassette (MSM) comprising a mammalian selectable marker gene; (c) an expression cassette (MASM) comprising a mammalian amplifiable, selectable marker gene; wherein the expression cassette (POI) is flanked 5? by the expression cassette (MASM), the expression cassette (MSM) is located 3? from the expression cassette (POI) and wherein the expression cassettes (MASM), (POI) and (MSM) are arranged in the same 5? to 3? orientation. Also provided are host cells, comprising said vector and methods for producing a polypeptide using respective host cells.
    Type: Application
    Filed: May 29, 2012
    Publication date: October 25, 2012
    Applicant: NOVARTIS AG
    Inventors: Hans-Peter Knopf, Burkhard Wilms
  • Publication number: 20110281751
    Abstract: The present invention inter alia pertains to a method for generating or selecting a eukaryotic host cell expressing a desired level of a polypeptide of interest, comprising: a) providing a plurality of eukaryotic host cells comprising a heterologous nucleic acid comprising at least one cassette (Cas-POI) comprising at least a first polynucleotide (Pn-POI) encoding the polypeptide of interest, at least one stop codon downstream of the first polynucleotide, and a second polynucleotide downstream of the stop codon encoding an immunoglobulin transmembrane anchor or a functional variant thereof; b) cultivating the eukaryotic host cells to allow expression of the polypeptide of interest such that at least a portion of the polypeptide of interest is expressed as a fusion polypeptide comprising the immunoglobulin transmembrane anchor or a functional variant thereof, wherein said fusion polypeptide is being displayed on the surface of said host cell; c) selecting at least one eukaryotic host cell based upon the pre
    Type: Application
    Filed: August 28, 2009
    Publication date: November 17, 2011
    Applicant: NOVARTIS AG
    Inventors: Thomas Jostock, Hans-Peter Knopf, Burkhard Wilms, Audrey Nommay
  • Publication number: 20110045536
    Abstract: The invention provides vector nucleic acid for expressing at least one polypeptide of interest in a mammalian cell, comprising (a) at least one expression cassette (POI) for expressing a polypeptide of interest; (b) an expression cassette (MSM) comprising a mammalian selectable marker gene; (c) an expression cassette (MASM) comprising a mammalian amplifiable, selectable marker gene; wherein the expression cassette (POI) is flanked 5? by the expression cassette (MASM), the expression cassette (MSM) is located 3? from the expression cassette (POI) and wherein the expression cassettes (MASM), (POI) and (MSM) are arranged in the same 5? to 3? orientation. Also provided are host cells, comprising said vector and methods for producing a polypeptide using respective host cells.
    Type: Application
    Filed: December 18, 2008
    Publication date: February 24, 2011
    Applicant: NOVARTIS AG
    Inventors: Hans-Peter Knopf, Burkhard Wilms