Abstract: Disclosed are: a method for detecting a target biomolecule in a biological sample, in which the method can be rapidly and inexpensively performed on site to diagnose diseases such as infectious diseases; an FO-LSPR optical fiber sensor which can be used therein; and a disposable sensor cartridge having the sensor mounted. The disposal sensor cartridge of the present invention can he used in the rapid detection of a target biomolecule, by mounting an optical fiber sensor and then connecting FO-LSPR equipment thereto. When an FO-LSPR molecular diagnostic system using the optical fiber sensor cartridge of the present invention is combined with a CRISPR-Cas system, target biomolecules can be detected accurately and conveniently without nucleic acid amplification.

Abstract: Provided are determining methods of human papillomavirus (HPV) genotypes with a high sensitivity. The method includes performing two-step PCRs on an HPV L1 gene in a sample to be analyzed as a biotin-labeled, single-stranded L1 gene, performing a hybridization reaction on the biotin-labeled, single-stranded L1 gene with a HPV genotype detection probe, reacting the hybridization reaction product with fluorescent substance combined with streptavidine, and measuring a fluorescent substance level to identify the HPV genotype. The detection method has high sensitivity enough to detect an extremely small amount of HPV in the sample. In addition, the high specificity exhibited by the detection method enables accurate diagnosis specific to HPV type.

Abstract: Provided are determining methods of human papillomavirus (HPV) genotypes with a high sensitivity. The method includes performing two-step PCRs on an HPV L1 gene in a sample to be analyzed as a biotin-labeled, single-stranded L1 gene, performing a hybridization reaction on the biotin-labeled, single-stranded L1 gene with a HPV genotype detection probe, reacting the hybridization reaction product with fluorescent substance combined with streptavidine, and measuring a fluorescent substance level to identify the HPV genotype. The detection method has high sensitivity enough to detect an extremely small amount of HPV in the sample. In addition, the high specificity exhibited by the detection method enables accurate diagnosis specific to HPV type.