Abstract: The present invention relates to a novel taxol-like protein (“TALP”), a process for preparing the same, and use of the same as anticancer and antiviral agents. TALP is prepared by a process which comprises the steps of homogenizing mammalian tissue, obtaining supernatant after centrifugation, and fractionating the supernatant thus obtained by cation-exchange chromatography and hydroxyapatite chromatography. TALP of the invention promotes tubulin polymerization without GTP, like taxol, and the microtubules thus polymerized are resistant to disassembly caused by Ca2+ and cold temperature under 4° C. and highly stabilized. Moreover, TALP of the invention inhibits cell proliferation, regeneration of scraped NIH 3T3 cell, proliferation and in vitro motility of HM7 human colon cancer cell, cell division of fertilized Xenopus oocyte, binding of tau protein to tubulin molecule, and E. coli lysis caused by virus.