Abstract: Provided are a microorganism for producing retinol, in which retinol biosynthetic genes are introduced; and a method of producing retinol, the method including a step of culturing the microorganism. The microorganism of the present invention may have an improved ability to produce retinol, and thus it may be efficiently used in producing retinol. Based on the method of producing retinol, the method including the step of culturing the microorganism, the retinol production efficiency may be improved.

Abstract: A recombinant microorganism comprising a lactic acid (LA) transporter, wherein the expression of the LA transporter in the recombinant microorganism is increased relative to a parent microorganism, and a method of producing lactic acid using same.

Abstract: A recombinant microorganism comprising a lactic acid (LA) transporter, wherein the expression of the LA transporter in the recombinant microorganism is increased relative to a parent microorganism, and a method of producing lactic acid using same.

Abstract: An expression vector which is capable of overexpressing a protein of interest in a host cell, a host cell comprising the expression vector, and a method of producing a protein of interest are provided.

Abstract: Provided herein are a recombinant vector and microorganism including the isolated SNR84 gene, and a method of increasing volumetric productivity of biofuel from a galactose-containing carbon source using the isolated SNR84 gene, the recombinant vector or the recombinant microorganism. Also disclosed herein is a method of screening yeast for genes associated with increased galactose catabolism when the genes are overexpressed.

Abstract: A method of increasing 3-HP production efficiency by inhibiting expression of a lactate dehydrogenase, a phosphotransacetylase, and an alcohol dehydrogenase in production of 3-HP using a malonic semialdehyde reduction pathway to prevent metabolite leak and increase a malonyl-CoA pool is disclosed.

Abstract: In a method of producing biofuel using brown algae, Bacterium antarctica is used as a hydrolysis catalyst for saccharification to obtain monosaccharides from the brown algae. The saccharification with the hydrolysis catalyst is effective in saccharification of the brown algae.

Abstract: A method of increasing 3-HP production efficiency by inhibiting expression of a lactate dehydrogenase, a phosphotransacetylase, and an alcohol dehydrogenase in production of 3-HP using a malonic semialdehyde reduction pathway to prevent metabolite leak and increase a malonyl-CoA pool is disclosed.

Abstract: A high throughput screening system and method of an acid-producing microorganism using a mixture of at least two pH indicators are provided. The method may be useful in determining a production amount of an acid, which is a final metabolite secreted by the microorganism, more accurately, rapidly and easily.

Abstract: A high throughput screening system and method of a lactic acid-producing microorganism using a mixture of at least two pH indicators are provided. The method may be useful in determining a production amount of a lactic acid, which is a final metabolite secreted by the microorganism, more accurately, rapidly and easily.

Abstract: A method of simultaneously producing 3-hydroxypropionic acid (3-HP) and 1,3-propanediol (1,3-PDO) using a microorganism is provided. The method includes converting glycerol into 3-HP and 1,3-PDO using a recombinant microorganism having both 3-HP and 1,3-PDO producing genes.

Abstract: A method of producing 3-hydroxypropionic acid (“3-HP”) with a high yield using a recombinant microorganism having an activity of reducing malonyl CoA into malonic semialdehyde and an activity of reducing malonic semialdehyde into 3-HP and/or an NADPH regeneration activity is provided.

Abstract: In a method of producing biofuel using brown algae, Bacterium antarctica is used as a hydrolysis catalyst for saccharification to obtain monosaccharides from the brown algae. The saccharification with the hydrolysis catalyst is effective in saccharification of the brown algae.

Abstract: Provided herein is an isolated polynucleotide for increasing the alcohol tolerance of a host cell. Also disclosed herein are a vector and a host cell containing the isolated polynucleotide, and a method of increasing the volumetric productivity of a bioalcohol using the same.

Abstract: Disclosed is a method for developing novel strains deleted specific chromosome sites, using transposon and Cre/loxP site-specific recombination by Cre expression vector, wherein the transposon comprises a selectable marker and loxP site. The method comprises the steps of: (1) preparing a transposon comprising a selectable marker and loxP site; (2) inserting the transposon into an optional position of microbial chromosome, and determining the inserted site; (3) integrating two transposons comprising a different selectable marker to one chromosome; (4) deleting a chromosomal site between the two lox sites by introducing a Cre expression vector into the chromosome of step (3); and (5) repeating steps (3 and 4) for the mutant deleted a part of chromosome, to shorten the chromosome of mutant gradually.

Abstract: Provided are a recombinant gene associated with increased galactose catabolism, and a recombinant vector and microorganism including the gene. Also disclosed are a method of producing ethanol from a galactose-containing carbon source by culturing the microorganism including the gene in a galactose-containing carbon source such that ethanol is produced, and a method of screening a gene in yeast resulting in increased galactose catabolism when overexpressed.

Abstract: Provided herein are a recombinant vector and microorganism including the isolated SNR84 gene, and a method of increasing volumetric productivity of biofuel from a galactose-containing carbon source using the isolated SNR84 gene, the recombinant vector or the recombinant microorganism. Also disclosed herein is a method of screening yeast for genes associated with increased galactose catabolism when the genes are overexpressed.

Abstract: Disclosed herein are a recombinant vector for deletion of specific chromosomal regions and a method for deletion of targeted microbial chromosomal regions using the same. Specifically, the recombinant vector comprises an arabinose-inducible promoter; a gene encoding a protein involved in lambda (?)-red recombination; a rhamnose-inducible promoter; and a gene encoding the I-SceI endonuclease. The present invention enables a convenient, rapid and markerless successive deletion of specific genes of microbes, as compared to a conventional method.

Abstract: Disclosed is a method for developing novel strains deleted specific chromosome sites, using transposon and Cre/loxP site-specific recombination by Cre expression vector, wherein the transposon comprises a selectable marker and loxP site. The method comprises the steps of: (1) preparing a transposon comprising a selectable marker and loxP site; (2) inserting the transposon into an optional position of microbial chromosome, and determining the inserted site; (3) integrating two transposons comprising a different selectable marker to one chromosome; (4) deleting a chromosomal site between the two lox sites by introducing a Cre expression vector into the chromosome of step (3); and (5) repeating steps (3 and 4) for the mutant deleted a part of chromosome, to shorten the chromosome of mutant gradually.

Abstract: The present invention relates to Escherichia coli variants that have increased antibiotics susceptibility, diffusion efficiency, and transformation efficiency. The variants can minimize the problems caused by biofilm formation such as increased resistance to antibiotics, decreased solute diffusion efficiency, and lowered transformation efficiency. According to the present invention, when selecting genetically-modified E. coli variants, not only a lesser amount of antibiotics is required when selecting desirable variants, but also the reduction of selection efficiency caused by biofilm formation by strains other than the variants to be selected, thus decreasing exhibiting resistance to antibiotics, can be avoided. Additionally, in the process of materials production, the amount of secreted products could be increased due to the increased solute diffusion efficiency. Furthermore, the increased transformation efficiency makes the mass production of useful materials easier.