Patents by Inventor Byung-Chun Yoo
Byung-Chun Yoo has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11920140Abstract: Provided herein are methods and systems for altering the genome of an organelle. In some embodiments, the method comprises introducing into an organelle a recombinant DNA construct comprising a first polynucleotide encoding at least one guide RNA and a second polynucleotide encoding a polynucleotide guided polypeptide; and growing a cell comprising the organelle under conditions in which the first polynucleotide and the second polynucleotide are each expressed.Type: GrantFiled: July 18, 2022Date of Patent: March 5, 2024Assignee: NAPIGEN, INC.Inventors: Hajime Sakai, Byung-Chun Yoo, Emil Meyer Orozco, Jr., Ganesh Kishore, Narendra Singh Yadav
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Publication number: 20230257771Abstract: The disclosure provides a polynucleotide comprising: a first DNA sequence encoding a guide RNA (gRNA), wherein the gRNA comprises a DNA-binding domain and a Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-associated endonuclease protein-binding domain, and the DNA-binding domain is complementary to a target sequence in an NRF2 gene; and a first promoter that is operably linked to the DNA sequence. The disclosure also provides vectors (e.g. AAV vectors), recombinant AAV (rAAV) and pharmaceutical compositions comprising the polynucleotides described herein. The disclosure further provides a method of treating cancer in a subject, the method comprising administering to the subject a therapeutically effective amount of a polynucleotide, vector, rAAV or pharmaceutical composition as described herein to the subject.Type: ApplicationFiled: April 20, 2021Publication date: August 17, 2023Applicant: Christiana Care Health Services, Inc.Inventors: Eric B. Kmiec, Byung-Chun Yoo, Natalia Rivera-Torres, Pawel Bialk
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Publication number: 20230123175Abstract: Provided herein are methods and systems for altering the genome of an organelle. In some embodiments, the method comprises introducing into an organelle a recombinant DNA construct comprising a first polynucleotide encoding at least one guide RNA and a second polynucleotide encoding a polynucleotide guided polypeptide; and growing a cell comprising the organelle under conditions in which the first polynucleotide and the second polynucleotide are each expressed.Type: ApplicationFiled: July 18, 2022Publication date: April 20, 2023Inventors: Hajime Sakai, Byung-Chun Yoo, Emil Meyer Orozco, JR., Ganesh Kishore, Narendra Singh Yadav
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Publication number: 20220249626Abstract: This disclosure relates to methods of and compositions for reducing expression or activity of a variant gene comprising at least one mutation as compared its wild-type gene, comprising introducing into a cell comprising the variant gene one or more DNA sequences encoding two or more gRNAs that are complementary to two or more target sequences in the variant gene, wherein at least one of the gRNAs hybridizes to a target sequence comprising a PAM site in the variant gene that results from a mutation to the variant gene creating the PAM site that does not exist in the wild-type gene or is operably linked to a mutated portion of the wild-type gene, at least one of the gRNAs hybridizes to a target sequence comprising a PAM site in an intron of the variant gene downstream or upstream from the PAM site, and a nucleic acid sequence encoding a CRISPR-associated endonuclease; wherein a CRISPR-associated endonuclease cleaves the variant gene at the target sequences; and expression or activity of the variant gene is reduType: ApplicationFiled: February 4, 2022Publication date: August 11, 2022Applicant: Christiana Care Health Services, Inc.Inventors: Eric B. Kmiec, Byung-Chun Yoo
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Publication number: 20210054404Abstract: Provided herein are methods and systems for altering the genome of an organelle. In some embodiments, the method comprises introducing into an organelle a recombinant DNA construct comprising a first polynucleotide encoding at least one guide RNA and a second polynucleotide encoding a polynucleotide guided polypeptide; and growing a cell comprising the organelle under conditions in which the first polynucleotide and the second polynucleotide are each expressed.Type: ApplicationFiled: August 22, 2018Publication date: February 25, 2021Inventors: Hajime Sakai, Byung-Chun Yoo, Emil Meyer Orozco, Jr., Roger Wyse, Ganesh Kishore, Jay Keasling, Narendra Singh Yadav
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Patent number: 10538778Abstract: This invention relates to polynucleotide sequences encoding SUT2 or SUT4 sucrose transporter genes. Methods for increasing seed oil content and evaluating increased oil content in a plant seed are described. The compositions and methods disclosed herein employ a variety of sequences that encode sucrose transporters and a variety of sequences that influence fatty acid accumulation, including for example, DGAT, Lec1 and ODP1 transcription factor. In specific embodiments, overexpression of SUT2 and/or SUT4 sucrose transporters in combination with DGAT genes further increase plant seed oil production compared to high oil plant comprising recombinant DNA constructs that do not overexpress SUT2 or SUT4 transporters.Type: GrantFiled: April 24, 2018Date of Patent: January 21, 2020Assignee: E I DU PONT DE NEMOURS AND COMPANYInventors: Stephen M Allen, Howard Glenn Damude, John D Everard, Knut Meyer, Byung-Chun Yoo
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Publication number: 20190136249Abstract: Provided herein are methods and systems for altering the genome of an organelle. In some embodiments, the method comprises introducing into an organelle a recombinant DNA construct comprising a first polynucleotide encoding at least one guide RNA and a second polynucleotide encoding a polynucleotide guided polypeptide; and growing a cell comprising the organelle under conditions in which the first polynucleotide and the second polynucleotide are each expressed.Type: ApplicationFiled: August 22, 2018Publication date: May 9, 2019Inventors: Hajime SAKAI, Byung-Chun YOO, Emil Meyer OROZCO, JR., Roger WYSE, Ganesh KISHORE, Jay KEASLING, Narendra Singh YADAV
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Publication number: 20180245092Abstract: This invention relates to polynucleotide sequences encoding SUT2 or SUT4 sucrose transporter genes. Methods for increasing seed oil content and evaluating increased oil content in a plant seed are described. The compositions and methods disclosed herein employ a variety of sequences that encode sucrose transporters and a variety of sequences that influence fatty acid accumulation, including for example, DGAT, Lec1 and ODP1 transcription factor. In specific embodiments, overexpression of SUT2 and/or SUT4 sucrose transporters in combination with DGAT genes further increase plant seed oil production compared to high oil plant comprising recombinant DNA constructs that do not overexpress SUT2 or SUT4 transporters.Type: ApplicationFiled: April 24, 2018Publication date: August 30, 2018Applicant: E. I. DU PONT DE NEMOURS AND COMPANYInventors: STEPHEN M. ALLEN, HOWARD GLENN DAMUDE, JOHN D. EVERARD, KNUT MEYER, BYUNG-CHUN YOO
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Patent number: 9976154Abstract: This invention relates to polynucleotide sequences encoding SUT2 or SUT4 sucrose transporter genes. Methods for increasing seed oil content and evaluating increased oil content in a plant seed are described. The compositions and methods disclosed herein employ a variety of sequences that encode sucrose transporters and a variety of sequences that influence fatty acid accumulation, including for example, DGAT, Lec1 and ODP1 transcription factor. In specific embodiments, overexpression of SUT2 and/or SUT4 sucrose transporters in combination with DGAT genes further increase plant seed oil production compared to a high oil plant comprising recombinant DNA constructs that do not overexpress SUT2 or SUT4 transporters.Type: GrantFiled: March 24, 2015Date of Patent: May 22, 2018Assignee: E. I. DU PONT DE NEMOURS AND COMPANYInventors: Stephen M Allen, Howard Glenn Damude, John D Everard, Knut Meyer, Byung-Chun Yoo
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Publication number: 20150218577Abstract: This invention relates to polynucleotide sequences encoding SUT2 or SUT4 sucrose transporter genes. Methods for increasing seed oil content and evaluating increased oil content in a plant seed are described. The compositions and methods disclosed herein employ a variety of sequences that encode sucrose transporters and a variety of sequences that influence fatty acid accumulation, including for example, DGAT, Lec1 and ODP1 transcription factor. In specific embodiments, overexpression of SUT2 and/or SUT4 sucrose transporters in combination with DGAT genes further increase plant seed oil production compared to a high oil plant comprising recombinant DNA constructs that do not overexpress SUT2 or SUT4 transporters.Type: ApplicationFiled: March 24, 2015Publication date: August 6, 2015Inventors: Stephen M Allen, Howard Glenn Damude, John D Everard, Knut Meyer, Byung-Chun Yoo
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Patent number: 8993840Abstract: This invention relates to polynucleotide sequences encoding SUT2 or SUT4 sucrose transporter genes. Methods for increasing seed oil content and evaluating increased oil content in a plant seed are described. The compositions and methods disclosed herein employ a variety of sequences that encode sucrose transporters and a variety of sequences that influence fatty acid accumulation, including for example, DGAT, Lec1 and ODP1 transcription factor. In specific embodiments, overexpression of SUT2 and/or SUT4 sucrose transporters in combination with DGAT genes further increase plant seed oil production compared to a high oil plant comprising recombinant DNA constructs that do not overexpress SUT2 or SUT4 transporters.Type: GrantFiled: November 1, 2010Date of Patent: March 31, 2015Assignee: E I du Pont de Nemours and CompayInventors: Stephen M. Allen, Howard Glenn Damude, John D. Everard, Knut Meyer, Byung-Chun Yoo
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Publication number: 20110126318Abstract: This invention relates to polynucleotide sequences encoding SUT2 or SUT4 sucrose transporter genes. Methods for increasing seed oil content and evaluating increased oil content in a plant seed are described. The compositions and methods disclosed herein employ a variety of sequences that encode sucrose transporters and a variety of sequences that influence fatty acid accumulation, including for example, DGAT, Lec1 and ODP1 transcription factor. In specific embodiments, overexpression of SUT2 and/or SUT4 sucrose transporters in combination with DGAT genes further increase plant seed oil production compared to a high oil plant comprising recombinant DNA constructs that do not overexpress SUT2 or SUT4 transporters.Type: ApplicationFiled: November 1, 2010Publication date: May 26, 2011Applicant: E.I. duPont de Nemours and CompanyInventors: Stephen M. Allen, Howard Glenn Damude, John D. Everard, Knut Meyer, Byung-Chun Yoo
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Publication number: 20070130653Abstract: Methods and compositions are provided for reducing the level of expression of a target polynucleotide in an organism. The methods and compositions selectively silence the target polynucleotide through the expression of a chimeric polynucleotide comprising the target for a sRNA (the trigger sequence) operably linked to a sequence corresponding to all or part of the gene or genes to be silenced. In this manner, the final target of silencing is an endogenous gene in the organism in which the chimeric polynucleotide is expressed. In a further embodiment, the miRNA target is that of a heterologous miRNA or siRNA, the latter of which is coexpressed in the cells at the appropriate developmental stage to provide silencing of the final target when and where desired. In a further embodiment, the final target may be a gene in a second organism, such as a plant pest, that feeds upon the organism containing the chimeric gene or genes.Type: ApplicationFiled: June 16, 2006Publication date: June 7, 2007Applicants: Pioneer Hi-Bred International, Inc., E.I. du Pont de Nemours and Company, Plant Bioscience LimitedInventors: David Baulcombe, Enno Krebbers, William Hitz, Kimberly Glassman, Milo Aukerman, Robert Williams, Byung-Chun Yoo
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Patent number: 7189892Abstract: The present invention provides a polynucleotide sequence encoding a component of the protein machinery involved in small RNA trafficking, Cucurbita maxima phloem small RNA-binding protein (CmPSRB 1), and the corresponding polypeptide sequence. The invention also provides genetic constructs and transgenic plants comprising the polynucleotide sequence encoding a phloem small RNA-binding protein to alter (e.g., prevent, reduce or elevate) non-cell autonomous signaling events in the plants involving small RNA metabolism. These signaling events are involved in a broad spectrum of plant physiological and biochemical processes, including, for example, systemic resistance to pathogens, responses to environmental stresses, e.g., heat, drought, salinity, and systemic gene silencing (e.g., viral infections).Type: GrantFiled: June 17, 2004Date of Patent: March 13, 2007Assignees: The Regents of the University of California, Agrigenesis Biosciences LimitedInventors: William J. Lucas, Byung-Chun Yoo, Tony J. Lough, Erika Varkonyi-Gasic
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Publication number: 20050050588Abstract: Systemic translocation of RNA exerts non-cell-autonomous epigenetic control over plant development and defense. The present invention provides a polynucleotide sequence encoding a component of the protein machinery involved in small RNA trafficking, Cucurbita maxima PHLOEM SMALL RNA-BINDING PROTEIN1 (CmPSRB1), and the corresponding polypeptide sequence. Homologs of the disclosed sequences exist in other plant species. The invention also provides polynucleotide sequences of phloem small RNAs involved in signaling. Methods are also provided for using these PSRB1 and small RNA sequences and genetic constructs comprising these sequences to alter (e.g., prevent, reduce or elevate) non-cell autonomous signaling events in plants involving small RNA metabolism. These signaling events are involved in a broad spectrum of plant physiological and biochemical processes, including, for example, flowering, coordination of plant growth and development, systemic resistance to pathogens, responses to environmental stresses, e.Type: ApplicationFiled: June 17, 2004Publication date: March 3, 2005Inventors: William Lucas, Byung-Chun Yoo, Tony Lough, Erika Varkonyi-Gasic