Abstract: The present invention relates to a method for diagnosing esthetic degradations of skin, in particular linked to pollution, in a subject, comprising a step (a) of determining, in a skin sample of the subject, the level of at least one marker chosen from the group constituted of (i) bacteria of the species Propionibacterim acnes, bacteria of the family Micrococcaceae, bacteria of the genus Brachybacterium, bacteria of the genus Brevibacterium, bacteria of the order Burkholderiales, bacteria of the genus Parococcus, bacteria of the family Rhodobacteraceae and bacteria of the genus Fusobacterium, and (ii) metabolites of these bacteria chosen from 3-hydroxy-3-methylglutarate, 3-methylglutarate/2-methylglutarate, 4-guanidinobutanoate, 4-imidazoleacetate, 5-oxoproline, aconitrate, adipate, alanine, alpha-cetoglutarate, arabonate/xylonate, azelate, beta-citrylglutamate, choline, cis-urocanate, citraconate/glutaconate, fructose, fumarate, gamma-glutamylalanine, gamma-glutamylglutamine, gamma-glutamylglycine, gamma-glu

Abstract: The present invention concerns a method, in particular in vitro, of diagnosing a chronic exposure of a subject, in particular of the skin of a subject, to pollution, comprising a step (a) of determining, in a skin sample of the subject, the level of at least one marker chosen from the group consisting of bacteria of the species Corynebacterium lipophiloflavum, Corynebacterium durum, Corynebacterium propinquum, Kytococcus sedentarius, Brevibacterium sp., Haemophilus parainfluenzae, Actinomyces oris, Neisseria sp., Abiotrophia defectiva, Alicycliphilus sp., Micrococcus luteus, Caulobacter sp., Streptococcus mitis/oralis/pneumoniae and Staphylococcus epidermidis.

Abstract: The present invention concerns a method for diagnosing early ageing of the skin in a subject, that in particular is pollution-related, comprising a step (a) of determining in a skin sample of the subject the level of at least one marker selected from the group constituted of fungi comprising a nucleic acid encoding an ITS1 («Internal Transcribed Spacer 1») region of sequence being at least 90% identical to sequence SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5 or SEQ ID NO: 6, and optionally fungi comprising a nucleic acid encoding an ITS1 region of sequence being at least 90% identical to sequence SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9 or SEQ ID NO: 10.

Abstract: The present invention relates to a method of diagnosing a pellicular state in a subject, to a screening method of identifying an antidandruff agent, and to kits and nucleic acid arrays useful for said methods.