Abstract: A solution phase synthesis method for preparing an oligonucleotide, wherein at least some of the reagents are solid supported. The method suitable for large-scale synthesis comprises coupling a protected compound with a nucleotide derivative having a protection group in the presence of a solid supported activator to give an elongated oligonucleotide with a P(III)-internucleotide bond; optionally processing the elongated oligonucleotide by capping by reaction with a solid supported capping agent and/or by oxidizing or sulfurizing by reaction of the oligonucleotide with a solid supported oxidizing or sulfurization reagent; and removing the protection group. The coupling may include reacting a 3?-protected compound of formula: with a nucleotide derivative having a 5?-protection group, or reacting a 5?-protected compound of formula with a nucleotide derivative having a 3?-protection group.

Abstract: A solution phase synthesis method for preparing an oligonucleotide, wherein at least some of the reagents are solid supported. The method suitable for large- scale synthesis comprises coupling a protected compound with a nucleotide derivative having a protection group in the presence of a solid supported activator to give an elongated oligonucleotide with a P(III)-internucleotide bond; optionally processing the elongated oligonucleotide by capping by reaction with a solid supported capping agent and/or by oxidizing or sulfurizing by reaction of the oligonucleotide with a solid supported oxidizing or sulfurization reagent; and removing the protection group. The coupling may include reacting a 3?-protected compound of formula: with a nucleotide derivative having a 5?-protection group, or reacting a 5?-protected compound of formula with a nucleotide derivative having a 3?-protection group.

Abstract: A solution phase synthesis method for preparing an oligonucleotide, wherein at least some of the reagents are solid supported. The method suitable for large-scale synthesis comprises coupling a protected compound with a nucleotide derivative having a protection group in the presence of a solid supported activator to give an elongated oligonucleotide with a P(III)-internucleotide bond; optionally processing the elongated oligonucleotide by capping by reaction with a solid supported capping agent and/or by oxidizing or sulfurizing by reaction of the oligonucleotide with a solid supported oxidizing or sulfurization reagent; and removing the protection group. The coupling may include reacting a 3?-protected compound of formula: with a nucleotide derivative having a 5?-protection group, or reacting a 5?-protected compound of formula with a nucleotide derivative having a 3?-protection group.

Abstract: The invention concerns a method for analyzing biological targets of DNA or RNA type, which comprises the following steps: a) contacting the targets to be analyzed (ICAM-20) with oligonucleotide probes (ISIS) labeled with a cofactor A of an enzyme E; b) adding, to the reaction medium, enzyme E corresponding to cofactor A and a substrate S of enzyme E, substrate S being converted by enzyme E into a compound C; c) measuring a signal representative of the activity of enzyme E on substrate S, for example the fluorescence intensity (I) of substrate S; and d) comparing this signal with the signal obtained when the oligonucleotide probes labeled with cofactor A (ISIS) are contacted with enzyme E and substrate S, under the same conditions, but in the absence of the targets, the difference between the two signals indicating the presence of complementary targets (ICAM-20) of the oligonucleotide probes.

Abstract: A method for preparing an oligonucleotide comprising the steps of a) providing a 3-protected compound having the formula: wherein B is a heterocyclic base R2 is H, a protected 2-hydroxyl group, F, a protected amino group, an O-alkyl group, an O-substituted alkyl, a substituted alkylamino or a C4?-O2?methylen linkage R3 is OR?3, NHR?3, NR?3R??3, a 3?-protected nucleotide or a 3?-protected oligonucleotide, R?3 is a hydroxyl protecting group, R?3, R??3 are independently an amine protecting group, b) reacting said compound with a nucleotide derivative having a 5-proctection group in the presence of a solid supported activator to give an elongated oligonucleotide with a P(III)-internucleotide bond c) optionally processing the elongated oligonucleotide with a P(III)-internucleotide bond by either or both of steps c1) and c2) in any sequence c1) capping preferably by reacting with a solid supported capping agent c2) oxidizing preferably by reacting the oligonucleotide with a solid supported oxidizing reagent d) remo

Abstract: This invention relates to an analytical support containing a plurality of oligonucleotides fixed on this support, where each of the nucleotides is marked by a fluorescent compound that presents a variation of fluorescence on the hybridization of each marked oligonucleotide with at complementary oligonucleotide. This support makes it possible to carry out an analysis of biological targets by measuring the variation of fluorescence in order to determine the hybridization of the targets with the oligonucleotides of the support.

Abstract: The invention concerns a method for analyzing biological targets of DNA or RNA type, which comprises the following steps: a) contacting the targets to be analyzed (ICAM-20) with oligonucleotide probes (ISIS) labeled with a cofactor A of an enzyme E; b) adding, to the reaction medium, enzyme E corresponding to cofactor A and a substrate S of enzyme E, substrate S being converted by enzyme E into a compound C; c) measuring a signal representative of the activity of enzyme E on substrate S, for example the fluorescence intensity (I) of substrate S; and d) comparing this signal with the signal obtained when the oligonucleotide probes labeled with cofactor A (ISIS) are contacted with enzyme E and substrate S, under the same conditions, but in the absence of the targets, the difference between the two signals indicating the presence of complementary targets (ICAM-20) of the oligonucleotide probes.

Abstract: This invention relates to an analytical support comprising a plurality of oligonucleotides fixed on this support, characterized in that each of said oligonucleotides is marked by a fluorescent compound that presents a variation of fluorescence on the hybridization of each marked oligonucleotide with a complementary oligonucleotide.