Abstract: The invention concerns a genetically modified microorganism expressing a functional type I or II RuBisCO enzyme and a functional phosphoribulokinase (PRK), and in which the glycolysis pathway is at least partially inhibited, said microorganism being genetically modified so as to produce an exogenous molecule and/or to overproduce an endogenous molecule. According to the invention, the oxidative branch of the pentose phosphate pathway may also be at least partially inhibited. The invention also concerns the use of such a genetically modified microorganism for the production or overproduction of a molecule of interest and processes for the synthesis or bioconversion of molecules of interest.

Abstract: The invention relates to a naturally hydrogen-oxidizing bacterium which is genetically modified to produce lactate from CO2, said bacterium being genetically modified to overexpress at least one gene encoding a lactate dehydrogenase, and to a process for producing lactate from CO2 using such a bacterium.

Abstract: The present technology relates to a method for generating crop yield traits. The method comprises identifying a multi-genic system associated with one or more gene of interest, wherein the one or more gene of interest is operatively linked to yield of crop. Identifying at least one combination of genes within the multi-genic system that yields an active multi-protein system of interest and generating mutational diversity on the at least one combination of genes to generate a library. The method further comprises screening the library to identify mutations yielding an improved activity of multi-protein system of interest in an expression system and introducing the screened mutations into the crop.

Abstract: Process for the production of methionine or its derivatives by culturing a microorganism in an appropriate culture medium comprising a source of carbon and a source of sulfur. The microorganism and/or the culture medium are modified in such way that the methionine/carbon source yield is increased. The isolation of methionine or its derivates from the fermentation medium is also described.

Abstract: The invention concerns a genetically modified microorganism expressing a functional type I or II RuBisCO enzyme and a functional phosphoribulokinase (PRK), and in which the glycolysis pathway is at least partially inhibited, said microorganism being genetically modified so as to produce an exogenous molecule and/or to overproduce an endogenous molecule. According to the invention, the oxidative branch of the pentose phosphate pathway may also be at least partially inhibited. The invention also concerns the use of such a genetically modified microorganism for the production or overproduction of a molecule of interest and processes for the synthesis or bioconversion of molecules of interest.

Abstract: The invention concerns a genetically modified microorganism expressing a functional type I or II RuBisCO enzyme and a functional phosphoribulokinase (PRK), and in which the non-oxidative branch of the pentose phosphate pathway is at least partially inhibited, said microorganism being genetically modified so as to produce an exogenous molecule and/or to overproduce an endogenous molecule. The invention also concerns the use of such a genetically modified microorganism for the production or overproduction of a molecule of interest and processes for the synthesis or bioconversion of molecules of interest.

Abstract: The invention relates to a method for recycling at least one plastic product, the method comprising degrading at least one polymer of the plastic product to monomers using an enzyme and recovering the resulting monomers. The method of the invention may be used for degrading, simultaneously or sequentially, at least two different polymers of the plastic product, and/or for recycling at least two plastic products.

Abstract: The invention concerns a microorganism which is genetically modified so as to i) synthesize an organic monomer by fermentation of a carbon source, and ii) depolymerize a polymer constituted at least by an organic monomer which it is capable of synthesizing. The invention also concerns a method for producing an organic monomer using a genetically modified microorganism of this type, as well as the coculture of this microorganism with another microorganism which is capable of synthesizing a polymer of interest.

Abstract: The present invention relates to a method for degrading a plastic containing non-biodegradable polymers comprising submitting said plastic to at least one enzyme for modifying a polymer of said plastic which has a methane potential less than 5 Nm3/t+/?20%, wherein at least one product resulting from the modification exhibits a methane potential greater than 10 Nm3/t+/?20%.

Abstract: The present invention concerns a method for the biological preparation of prenol comprising culturing a microorganism genetically modified for the bioproduction of prenol, wherein the microorganism comprises a metabolic pathway for conversion of 3-methylcrotonyl-CoA into prenol by the action of an alcohol dehydrogenase enzyme and of an aldehyde dehydrogenase enzyme.

Abstract: The present invention relates to a method for the production of methionine using modified strains with attenuated transformation of threonine. This can be achieved by reducing threonine transformation into glycine, and/or by reducing its transformation to ?-ketobutyrate. The invention also concerns the modified strains with attenuated transformation of threonine.

Abstract: Process for the production of methionine or its derivatives by culturing a microorganism in an appropriate culture medium comprising a source of carbon and a source of sulfur. The microorganism and/or the culture medium are modified in such way that the methionine/carbon source yield is increased. The isolation of methionine or its derivates from the fermentation medium is also described.

Abstract: The invention relates to a method for recycling at least one plastic product, the method comprising degrading at least one polymer of the plastic product to monomers using an enzyme and recovering the resulting monomers. The method of the invention may be used for degrading, simultaneously or sequentially at least two different polymers of the plastic product, and/or for recycling at least two plastic products.

Abstract: The present invention concerns a new method for the biological preparation of a diol comprising culturing a microorganism genetically modified for the bioproduction of an aliphatic diol, wherein the microorganism comprises a metabolic pathway for the decarboxylation of a hydroxy-2-keto-aliphatic acid metabolite with an enzyme having a 2-keto acid decarboxylase activity, the product obtained from said decarboxylation step being further reduced into the corresponding aliphatic diol, and wherein the microorganism is genetically modified for the improved production of said hydroxy-2-keto-aliphatic acid metabolite. The invention also concerns a modified microorganism for the production of an aliphatic diol.

Abstract: The present invention is related to a microorganism for the production of methionine, wherein said microorganism is modified to enhance the transhydrogenase activity of PntAB. In a preferred aspect of the invention, the activity of the transhydrogenase UdhA is attenuated in said microorganisms. The invention also related to a method for producing methionine by fermentation.

Abstract: The invention concerns a microorganism which is genetically modified so as to i) synthesize a hydrocarbon monomer by fermentation of a carbon source, and ii) depolymerize a polymer constituted at least by hydrocarbon monomer which it is capable of synthesizing. The invention also concerns a method for producing a hydrocarbon monomer using a genetically modified microorganism of this type, as well as the coculture of this microorganism with another microorganism which is capable of synthesizing a polymer of interest.

Abstract: The present invention concerns a modified microorganism for the biological preparation of an hydroxy acid of formula (I) wherein the microorganism comprises a two-step metabolic pathway for the production of the said hydroxy acid from a hydroxy keto-acid of formula (II) through an intermediate hydroxy-aldehyde of formula (III), wherein EA1 is an enzyme having a 2-keto-acid decarboxylase activity, and EA2 is an enzyme having hydroxy aldehyde dehydrogenase activity. The invention also concerns a method for the fermentative production of a hydroxy acid.

Abstract: A microorganism genetically modified for the bioproduction of 1,3-propanediol from sucrose, wherein the microorganism includes: a two-step metabolic pathway for the production of 1,3-propanediol, including a first step of decarboxylation of 4-hydroxy-2-ketobutyrate with an enzyme having a 2-keto acid decarboxylase activity, and a second step of reduction of the obtained 3-hydroxypropionaldehyde with an enzyme having hydroxy aldehyde reductase activity, and genes enabling the microorganism to utilize sucrose as sole carbon source. A method for the biological preparation of 1,3-propanediol by fermentation, including cultivating said microorganism genetically modified, wherein the culture is performed in an appropriate medium including a source of sucrose, and recovering the 1,3-propanediol being produced.

Abstract: The present invention concerns a method for the biological preparation of prenol comprising culturing a microorganism genetically modified for the bioproduction of prenol, wherein the microorganism comprises a metabolic pathway for conversion of 3-methylcrotonyl-CoA into prenol by the action of an alcool dehydrogenase enzyme and of an aldehyde dehydrogenase enzyme.

Abstract: The present invention concerns a microorganism genetically modified for the bioproduction of 1,3-propanediol from sucrose, wherein the microorganism comprises: a two-step metabolic pathway for the production of 1,3-propanediol, comprising a first step of decarboxylation of 4-hydroxy-2-ketobutyrate with an enzyme having a 2-keto acid decarboxylase activity, and a second step of reduction of the obtained 3 -hydroxypropionaldehyde with an enzyme having hydroxy aldehyde reductase activity, and genes enabling the microorganism to utilize sucrose as sole carbon source. The invention also concerns a new method for the biological preparation of 1,3-propanediol by fermentation, comprising cultivating said microorganism genetically modified, wherein the culture is performed in an appropriate medium comprising a source of sucrose, and recovering the 1,3 -propanediol being produced. In a preferred aspect of the invention, the source of sucrose is obtained from plant biomass.