Abstract: The present invention relates to the use of at least one azo compound for detecting at least one microorganism in a sample. More precisely, the present invention relates to a process for detecting, in a biological sample, at least one microorganism with azoreductase activity, including the steps: placing the sample in contact with a reaction medium including at least one azo compound, incubating the reaction medium, and detecting the reduction of the azo compound by the microorganism, indicating the presence of the at least one microorganism.

Abstract: Embodiments disclosed herein relate to methods and systems for performing automated assays, and particularly to performing sequential assays on a sample on an automated instrument.

Abstract: Methods and kits useful for the detection and identification of carbapenem-resistant pathogens harboring carbapenemase-encoding nucleic acids.

Abstract: Provided herein are compositions and methods for the detection and identification of Staphylococcus aureus strains harboring polymorphic SCCmec right extremity (MREP) type xxi sequences.

Abstract: The present invention relates to a method for identifying at least two groups of microorganisms expressing the same enzymatic activity, comprising the following steps: a) incubating said groups of microorganisms in a reaction medium comprising a first enzyme substrate and a second enzyme substrate, said first and second enzyme substrates being metabolized by the same enzymatic activity; b) identifying said groups of microorganisms.

Abstract: The invention relates to a method for detecting and/or identifying Escherichia coli (E. coli) in a biological sample, that comprises inoculating the biological sample liable to contain E. coli on a detection medium that comprises tryptophan and a substrate for an enzyme A, expressed by the majority of E. coli, in order to obtain bacterial colonies; detecting the colonies expressing the activity of the enzyme A and identifying them as being E. coli; and detecting the colonies that do not express the activity of the enzyme A, carrying out an indole test, and identifying the colonies having a positive indole test as being E. coli.

Abstract: A method for distinguishing among a first group of microorganisms, belonging to a first taxon of yeasts that is resistant to an antifungal; a second group of microorganisms, belonging to a second taxon of yeasts that is different than the first taxon of yeasts, and that exhibits the mechanism of resistance to the antifungal exhibited by the first group; and a third group that is not resistant to the antifungal.

Abstract: A method for distinguishing among a first group of microorganisms belonging to a first taxon of Gram negative bacteria, the first group of bacteria exhibiting a mechanism of resistance to a treatment; a second group of microorganisms belonging to a second taxon of Gram negative bacteria, the second taxon of bacteria being different than said first taxon, and exhibiting a mechanism of resistance to a treatment identical to the mechanism of the first group; and a third group of Gram negative bacteria that is not resistant to the treatment.

Abstract: A method for distinguishing among a first group of microorganisms, belonging to a first taxon of bacteria that is vancomycin resistant; a second group of microorganisms, belonging to a second taxon of vancomycin resistant bacteria that is different than said first taxon; and a third group of microorganisms that is not resistant or has a natural resistance to vancomycin.

Abstract: A detection medium including a substrate for a metabolic activity specific for a group of Gram-negative bacteria, a beta-glucosidase or cellobiosidase substrate, and a beta-glucosidase or cellobiosidase inducer. The inducer is a carbohydrate constituted of a carbohydrate linked in the ?-position to glucose, or a carbohydrate with a ?glucoside subunit, preferably selected from cellobiose, cellulose, starch, cellotriose, and trehalose.

Abstract: Embodiments disclosed herein relate to compositions for the detection and/or identification of microbes that carry extended spectrum beta-lactamase genes. Specifically, provided herein are oligonucleotides, probes, and kits containing the same, for the detection of bacterial CTX-M sequences. Also provided are methods for the detection and/or amplification of microbes harboring extended spectrum beta-lactamase genes, including CTX-M type extended spectrum beta-lactamase genes.

Abstract: The invention relates to a method for detecting and/or identifying E. coli in a urine sample, that comprises: a) inoculating the urine sample liable to contain E. coli on a detection medium that comprises a first substrate selected from a beta-glucuronidase substrate, beta-galactosidase substrate and alpha-galactosidase substrate, and a substrate for a lactose acidification enzyme, beta-ribosidase, phosphatase, L-alanine aminopeptidase and L-leucine aminopeptidase, and a second substrate, different from said first substrate and selected from a beta-glucuronidase substrate, beta-galactosidase substrate and alpha-galactosidase substrate, and a substrate for a lactose acidification enzyme, beta-ribosidase, phosphatase, L-alanine aminopeptidase and L-leucine aminopeptidase, or obtaining bacterial colonies; b) identifying the colonies that react with the first substrate and/or the second substrate as being colonies of E. coli.

Abstract: The invention relates to a medium for detecting and/or identifying microorganisms present in a sample, comprising a culture medium and at least one substrate that can be hydrolysed to a labelled product by at least a first enzyme not free in the sample, and specific for the microorganisms, characterized in that it also comprises at least one inhibitor of at least a second enzyme, different from the first enzyme or identical to it, but free in said sample and not originating from a microorganism. The present invention also finds a preferred application in the field of biomedical diagnosis or in food microbiology, and more particularly in bacteriology and mycology.

Abstract: The invention relates to a method for detecting and/or identifying Escherichia coli (E. coli) in a biological sample, that comprises inoculating the biological sample liable to contain E. coli on a detection medium that comprises tryptophan and a substrate for an enzyme A, expressed by the majority of E. coli, in order to obtain bacterial colonies; detecting the colonies expressing the activity of the enzyme A and identifying them as being E. coli; and detecting the colonies that do not express the activity of the enzyme A, carrying out an indole test, and identifying the colonies having a positive indole test as being E. coli.

Abstract: A detection medium including a substrate for a metabolic activity specific for a group of Gram-negative bacteria a beta-glucosidase or cellobiosidase substrate and a beta-glucosidase or cellobiosidase inducer. The inducer is a carbohydrate constituted of a carbohydrate linked in the ?-position to glucose, or a carbohydrate with a ? glucoside subunit, preferably selected from cellobiose, cellulose, starch, cellotriose and trehalose.

Abstract: The present invention relates to a method for identifying at least two groups of microorganisms expressing the same enzymatic activity, comprising the following steps: a) incubating said groups of microorganisms in a reaction medium comprising a first enzyme substrate and a second enzyme substrate, said first and second enzyme substrates being metabolized by the same enzymatic activity; b) identifying said groups of microorganisms.

Abstract: A substrate which can be used for the direct identification of pathogenic bacteria belonging to the genus Listeria by detecting an esterase activity other than Phosphatidyl Inositol-specific Phospholipase C (PI-PLC), an esterase which is specific to the species Listeria monocytogenes. The use of two substrates, one as described above and the other specific for all or some members of the genus Listeria, and a reaction medium containing such a substrate or such a combination of substrates are disclosed. An identification method which exploits such culture media is also disclosed. The invention is particularly applicable in the field of diagnosis.

Abstract: The invention relates to the use of a combination of two culture media for distinguishing at least three groups of microorganisms in a biological sample, comprising: a first group of microorganisms, belonging to a first taxon of microorganisms and comprising at least a first mechanism of resistance to a first treatment; a second group of microorganisms, belonging to a second taxon of microorganisms and comprising at least a second mechanism of resistance to a second treatment; a third group of microorganisms, that are not resistant to said first and second treatments, said combination of two culture media comprising: a. at least a first substrate for detecting at least a first enzymatic or metabolic activity of said first group of microorganisms; b. at least two markers for differentiating the first group of microorganisms and the second group of microorganisms; c. at least one antimicrobial that is active on said third group of microorganisms.

Abstract: A substrate which can be used for the direct identification of pathogenic bacteria belonging to the genus Listeria by detecting an esterase activity other than Phosphatidyl Inositol-specific Phospholipase C (PI-PLC), an esterase which is specific to the species Listeria monocytogenes. The use of two substrates, one as described above and the other specific for all or some members of the genus Listeria, and a reaction medium containing such a substrate or such a combination of substrates are disclosed. An identification method which exploits such culture media is also disclosed. The invention is particularly applicable in the field of diagnosis.

Abstract: The invention relates to a method and a medium for microbiological analysis by biochemical means involving chromogenic or fluorogenic substrates that react with enzymes (esterases) specific for the target strains. One object of the invention is to improve the sensitivity, initial translucence, stability and ease of use of such detection/identification media. For this purpose the medium according to the invention is characterized in that it is in a stable, ready-to-use liquid or gel form and in that it contains a solubilizer and stabilizer selected from fatty acid sorbitan esters, bile salts and mixtures thereof, as well as a selective activator selected from alkylsulfate salts, for example the sodium salts. The medium can comprise a solvent, for example dimethyl sulfoxide. The invention further relates to the method of obtaining this medium and to the use of products of the sodium alkylsulfate type as selective activators in such media. Application: microbiological analyses.