Patents by Inventor Cagri A. Savran
Cagri A. Savran has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11478797Abstract: A microfluidic detection system for micrometer-sized entities, such as biological cells, includes a detector component incorporating a plate with a plurality of opening, the plate separating two chambers, one in communication with a fluid source containing target cells bound to magnetic beads. The openings are sized to always permit passage of the magnetic beads therethrough into a lower one of the chambers and are further sized to always prevent passage of the target cells from the upper one of the chambers. The detector component further includes a magnet positioned to pull unbound magnetic beads through the openings and to capture target cells bound to magnetic beads on the surface of the plate. The microfluidic detection system includes a pump flowing the fluid through the detector component at high flow rates of milliliters per minute for high throughput detection of target cells.Type: GrantFiled: May 23, 2019Date of Patent: October 25, 2022Assignee: Purdue Research FoundationInventors: Cagri A. Savran, Philip S. Low, Chun-Li Chang
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Publication number: 20220055034Abstract: Microfluidic systems and methods are described for capturing magnetic target entities bound to one or more magnetic beads. The systems include a well array device that includes a substrate with a surface that has a plurality of wells arranged in one or more arrays on the surface. A first array of wells is arranged adjacent to a first location on the surface. A second and subsequent arrays, if present, are arranged sequentially on the surface at second and subsequent locations. When a liquid sample is added onto the substrate and caused to flow, the liquid sample will flow across the first array first and then flow across the second and subsequent arrays in sequential order. The wells in the first array each have a size that permits entry of only one target entity into the well and each well in the first array has approximately the same size.Type: ApplicationFiled: October 27, 2021Publication date: February 24, 2022Inventors: Cagri A. Savran, Chun-Li Chang, Wanfeng Huang, Onur Gur, Rohil Jain
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Publication number: 20220016630Abstract: A microfluidic detection system for micrometer-sized entities, such as biological cells, includes a detector component incorporating a plate with a plurality of opening, the plate separating two chambers, one in communication with a fluid source containing target entities bound to magnetic beads. The openings are sized to always permit passage of the magnetic beads therethrough into a lower one of the chambers and are further sized to always prevent passage of the target entities from the upper one of the chambers. The detector component further includes a magnet positioned to pull unbound magnetic beads through the openings and to capture target entities bound to magnetic beads on the surface of the plate. In a further feature, the microfluidic detection system is configured to pass target molecules through the plate to be bound to a functionalized surface of the lower chamber.Type: ApplicationFiled: July 30, 2021Publication date: January 20, 2022Inventors: Cagri A. Savran, Philip S. Low, Chun-Li Chang, Wanfeng Huang
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Publication number: 20210380934Abstract: The application relates to methods and systems for culturing individually selected cells in relative isolation from the rest of a population of cells, under physiologically relevant and controllable environmental conditions that can be designed to mimic specific environments, e.g., within a human body.Type: ApplicationFiled: April 8, 2021Publication date: December 9, 2021Inventors: Cagri A. Savran, Rohil Jain, Chun-Li Chang
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Patent number: 11173488Abstract: Microfluidic systems and methods are described for capturing magnetic target entities bound to one or more magnetic beads. The systems include a well array device that includes a substrate with a surface that has a plurality of wells arranged in one or more arrays on the surface. A first array of wells is arranged adjacent to a first location on the surface. A second and subsequent arrays, if present, are arranged sequentially on the surface at second and subsequent locations. When a liquid sample is added onto the substrate and caused to flow, the liquid sample will flow across the first array first and then flow across the second and subsequent arrays in sequential order. The wells in the first array each have a size that permits entry of only one target entity into the well and each well in the first array has approximately the same size.Type: GrantFiled: April 24, 2017Date of Patent: November 16, 2021Assignee: Purdue Research FoundationInventors: Cagri A. Savran, Chun-Li Chang, Wanfeng Huang, Onur Gur, Rohil Jain
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Patent number: 11077439Abstract: A microfluidic detection system for micrometer-sized entities, such as biological cells, includes a detector component incorporating a plate with a plurality of opening, the plate separating two chambers, one in communication with a fluid source containing target entities bound to magnetic beads. The openings are sized to always permit passage of the magnetic beads therethrough into a lower one of the chambers and are further sized to always prevent passage of the target entities from the upper one of the chambers. The detector component further includes a magnet positioned to pull unbound magnetic beads through the openings and to capture target entities bound to magnetic beads on the surface of the plate. In a further feature, the microfluidic detection system is configured to pass target molecules through the plate to be bound to a functionalized surface of the lower chamber.Type: GrantFiled: February 14, 2019Date of Patent: August 3, 2021Assignee: Purdue Research FoundationInventors: Cagri A. Savran, Philip S. Low, Chun-Li Chang, Wanfeng Huang
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Publication number: 20200109446Abstract: Disclosed herein is a method and system for a high-throughput, quantitative analysis of protein-DNA interactions on synthetic and genomic DNA. This system and method makes use of sequencing chips which have already been used to carry out sequencing and is therefore environmentally friendly, as well as efficient and accurate.Type: ApplicationFiled: June 14, 2018Publication date: April 9, 2020Inventors: Ilya FINKELSTEIN, Cheulhee JUNG, John HAWKINS, Stephen Knox JONES, James RYBARSKI, Fatema A. SAIFUDDIN, Cagri SAVRAN, Andrew D. ELLINGTON, William H. PRESS
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Publication number: 20190344277Abstract: A microfluidic detection system for micrometer-sized entities, such as biological cells, includes a detector component incorporating a plate with a plurality of opening, the plate separating two chambers, one in communication with a fluid source containing target cells bound to magnetic beads. The openings are sized to always permit passage of the magnetic beads therethrough into a lower one of the chambers and are further sized to always prevent passage of the target cells from the upper one of the chambers. The detector component further includes a magnet positioned to pull unbound magnetic beads through the openings and to capture target cells bound to magnetic beads on the surface of the plate. The microfluidic detection system includes a pump flowing the fluid through the detector component at high flow rates of milliliters per minute for high throughput detection of target cells.Type: ApplicationFiled: May 23, 2019Publication date: November 14, 2019Inventors: Cagri A. Savran, Philip S. Low, Chun-Li Chang
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Publication number: 20190291109Abstract: A microfluidic detection system for micrometer-sized entities, such as biological cells, includes a detector component incorporating a plate with a plurality of opening, the plate separating two chambers, one in communication with a fluid source containing target entities bound to magnetic beads. The openings are sized to always permit passage of the magnetic beads therethrough into a lower one of the chambers and are further sized to always prevent passage of the target entities from the upper one of the chambers. The detector component further includes a magnet positioned to pull unbound magnetic beads through the openings and to capture target entities bound to magnetic beads on the surface of the plate. In a further feature, the microfluidic detection system is configured to pass target molecules through the plate to be bound to a functionalized surface of the lower chamber.Type: ApplicationFiled: February 14, 2019Publication date: September 26, 2019Inventors: Cagri A. Savran, Philip S. Low, Chun-Li Chang, Wanfeng Huang
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Publication number: 20190143328Abstract: Microfluidic systems and methods are described for capturing magnetic target entities bound to one or more magnetic beads. The systems include a well array device that includes a substrate with a surface that has a plurality of wells arranged in one or more arrays on the surface. A first array of wells is arranged adjacent to a first location on the surface. A second and subsequent arrays, if present, are arranged sequentially on the surface at second and subsequent locations. When a liquid sample is added onto the substrate and caused to flow, the liquid sample will flow across the first array first and then flow across the second and subsequent arrays in sequential order. The wells in the first array each have a size that permits entry of only one target entity into the well and each well in the first array has approximately the same size.Type: ApplicationFiled: April 24, 2017Publication date: May 16, 2019Inventors: Cagri A. Savran, Chun-Li Chang, Wanfeng Huang, Onur GUR, Rohil JAIN
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Patent number: 10246698Abstract: An intermediate composite capable of transferring a biological or chemical material to be patterned on a surface. The intermediate composite includes a hydrogel, and particles suspended in the hydrogel, generating a particle-gel composite (composite), the composite is configured to absorb a biological or chemical material (agent), and further configured to deposit the agent when the composite is positioned proximate to a surface on which the agent is to be deposited.Type: GrantFiled: December 25, 2017Date of Patent: April 2, 2019Assignee: Purdue Research FoundationInventors: Cagri A. Savran, Bin-Da Chan, Richard Lee Gieseck, III
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Patent number: 10207267Abstract: A microfluidic detection system for micrometer-sized entities, such as biological cells, includes a detector component incorporating a plate with a plurality of opening, the plate separating two chambers, one in communication with a fluid source containing target entities bound to magnetic beads. The openings are sized to always permit passage of the magnetic beads therethrough into a lower one of the chambers and are further sized to always prevent passage of the target entities from the upper one of the chambers. The detector component further includes a magnet positioned to pull unbound magnetic beads through the openings and to capture target entities bound to magnetic beads on the surface of the plate. In a further feature, the microfluidic detection system is configured to pass target molecules through the plate to be bound to a functionalized surface of the lower chamber.Type: GrantFiled: November 18, 2016Date of Patent: February 19, 2019Assignee: Purdue Research FoundationInventors: Cagri A. Savran, Philip S. Low, Chun-Li Chang, Wanfeng Huang
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Publication number: 20180348213Abstract: Additive techniques, including a direct-dilution method and a direct-incubation method, are described for isolating target entities in a fluid sample. In some implementations, a volume of a diluent is added to a fluid sample to generate a first mixture. The volume of the diluent added may be sufficient to obtain a specified viscosity of the first mixture lower than a viscosity of the fluid sample. A number of binding moiety-conjugated magnetic beads are added to the first mixture to generate a second mixture. The second mixture is incubated for a time that is sufficient for the binding moiety-conjugated magnetic beads to bind to rare target entities in the second mixture. A portion of the second mixture is injected into a fluidic chamber. A magnetic force is applied to attract the magnetized rare target entities in the second mixture to an isolation surface within the fluidic chamber.Type: ApplicationFiled: September 22, 2016Publication date: December 6, 2018Inventors: Cagri A. Savran, Chun-Li Chang, Wanfeng Huang
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Publication number: 20180148709Abstract: An intermediate composite capable of transferring a biological or chemical material to be patterned on a surface. The intermediate composite includes a hydrogel, and particles suspended in the hydrogel, generating a particle-gel composite (composite), the composite is configured to absorb a biological or chemical material (agent), and further configured to deposit the agent when the composite is positioned proximate to a surface on which the agent is to be deposited.Type: ApplicationFiled: December 25, 2017Publication date: May 31, 2018Applicant: Purdue Research FoundationInventors: Cagri A. Savran, Bin-Da Chan, Richard Lee Gieseck, III
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Publication number: 20180148708Abstract: An intermediate composite capable of transferring a biological or chemical material to be patterned on a surface. The intermediate composite includes a hydrogel, and particles suspended in the hydrogel, generating a particle-gel composite (composite), the composite is configured to absorb a biological or chemical material (agent), and further configured to deposit the agent when the composite is positioned proximate to a surface on which the agent is to be deposited.Type: ApplicationFiled: December 25, 2017Publication date: May 31, 2018Applicant: Purdue Research FoundationInventors: Cagri A. Savran, Bin-Da Chan, Richard Lee Gieseck, III
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Patent number: 9850478Abstract: An intermediate composite capable of transferring a biological or chemical material to be patterned on a surface. The intermediate composite includes a hydrogel, and particles suspended in the hydrogel, generating a particle-gel composite (composite), the composite is configured to absorb a biological or chemical material (agent), and further configured to deposit the agent when the composite is positioned proximate to a surface on which the agent is to be deposited.Type: GrantFiled: August 12, 2015Date of Patent: December 26, 2017Assignee: Purdue Research FoundationInventors: Cagri A. Savran, Bin-Da Chan, Richard Lee Gieseck, III
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Publication number: 20170131283Abstract: A microfluidic detection system for micrometer-sized entities, such as biological cells, includes a detector component incorporating a plate with a plurality of opening, the plate separating two chambers, one in communication with a fluid source containing target entities bound to magnetic beads. The openings are sized to always permit passage of the magnetic beads therethrough into a lower one of the chambers and are further sized to always prevent passage of the target entities from the upper one of the chambers. The detector component further includes a magnet positioned to pull unbound magnetic beads through the openings and to capture target entities bound to magnetic beads on the surface of the plate. In a further feature, the microfluidic detection system is configured to pass target molecules through the plate to be bound to a functionalized surface of the lower chamber.Type: ApplicationFiled: November 18, 2016Publication date: May 11, 2017Inventors: Cagri A. Savran, Philip S. Low, Chun-Li Chang, Wanfeng Huang
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Patent number: 9500625Abstract: A microfluidic detection system for micrometer-sized entities, such as biological cells, includes a detector component incorporating a plate with a plurality of opening, the plate separating two chambers, one in communication with a fluid source containing target entities bound to magnetic beads. The openings are sized to always permit passage of the magnetic beads therethrough into a lower one of the chambers and are further sized to always prevent passage of the target entities from the upper one of the chambers. The detector component further includes a magnet positioned to pull unbound magnetic beads through the openings and to capture target entities bound to magnetic beads on the surface of the plate. In a further feature, the microfluidic detection system is configured to pass target molecules through the plate to be bound to a functionalized surface of the lower chamber.Type: GrantFiled: September 26, 2013Date of Patent: November 22, 2016Assignee: Purdue Research FoundationInventors: Cagri A. Savran, Philip S. Low, Chun-Li Chang, Wanfeng Huang
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Patent number: 9494557Abstract: A microfluidic detection system for micrometer-sized entities, such as biological cells, includes a detector component incorporating a plate with a plurality of opening, the plate separating two chambers, one in communication with a fluid source containing target cells bound to magnetic beads. The openings are sized to always permit passage of the magnetic beads therethrough into a lower one of the chambers and are further sized to always prevent passage of the target cells from the upper one of the chambers. The detector component further includes a magnet positioned to pull unbound magnetic beads through the openings and to capture target cells bound to magnetic beads on the surface of the plate. The microfluidic detection system includes a pump flowing the fluid through the detector component at high flow rates of milliliters per minute for high throughput detection of target cells.Type: GrantFiled: April 5, 2012Date of Patent: November 15, 2016Assignee: Purdue Research FoundationInventors: Cagri A. Savran, Chung-Li Chang, Philip S. Low
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Patent number: 9341621Abstract: Systems and methods for rapid and ultrasensitive detection of target biomolecules in a sample are presented. The detection of biomolecules is achieved through a synergistic use of immunoseparation and diffractometry, and the formation of antibody-biomolecule-ligand sandwich complexes that form diffraction gratings. Characteristic diffraction patterns are then produced upon illumination of the diffraction gratings with light. The diffraction patterns can be used to detect very low amounts of biomolecules present in the sample.Type: GrantFiled: May 29, 2008Date of Patent: May 17, 2016Assignee: Purdue Research FoundationInventors: Cagri Savran, Philip Low, David Thompson, Walter Henne, Derek Doorneweerd, Chun-Li Chang, Ghanashyam Acharya, David Brownholland